دورية أكاديمية
Intravital Microscopy in Atherosclerosis Research.
| العنوان: | Intravital Microscopy in Atherosclerosis Research. |
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| المؤلفون: | Wissmeyer G; Cardiovascular Research Center, Division of Cardiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Kassab MB; Cardiovascular Research Center, Division of Cardiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Kawamura Y; Cardiovascular Research Center, Division of Cardiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA., Aguirre AD; Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA., Jaffer FA; Cardiovascular Research Center, Division of Cardiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. fjaffer@mgh.harvard.edu.; Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA. fjaffer@mgh.harvard.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2419, pp. 645-658. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Atherosclerosis* , Microscopy, Fluorescence, Multiphoton*/methods, Animals ; Intravital Microscopy/methods ; Mice ; Photons |
| مستخلص: | Atherosclerosis is a lipid-driven inflammatory disorder that narrows the arterial lumen and can induce life-threatening complications from coronary artery disease, cerebrovascular disease, and peripheral artery disease. On a mechanistic level, the development of novel cellular-resolution intravital microscopy imaging approaches has recently enabled in vivo studies of underlying biological processes governing disease onset and progress. In particular, multiphoton microscopy has emerged as a promising intravital imaging tool utilizing two-photon-excited fluorescence and second-harmonic generation that provides subcellular resolution and increased imaging depths beyond confocal and epifluorescence microscopy. In this chapter, we describe the state-of-the-art multiphoton microscopy applied to the study of murine atherosclerosis. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| معلومات مُعتمدة: | R01 HL137913 United States HL NHLBI NIH HHS; R01 HL144515 United States HL NHLBI NIH HHS; R01 HL150538 United States HL NHLBI NIH HHS |
| فهرسة مساهمة: | Keywords: Atherosclerosis; Gated microscopy; Inflammation; Intravital imaging; Intravital microscopy; Molecular imaging; Multiphoton microscopy |
| تواريخ الأحداث: | Date Created: 20220303 Date Completed: 20220304 Latest Revision: 20230102 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9052099 |
| DOI: | 10.1007/978-1-0716-1924-7_40 |
| PMID: | 35237994 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-1924-7_40 |