دورية أكاديمية
Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease.
| العنوان: | Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease. |
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| المؤلفون: | Hallett JM; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Ferreira-Gonzalez S; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Man TY; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Kilpatrick AM; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Esser H; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Thirlwell K; Tissues, Cells and Advanced Therapeutics Scottish National Blood and Transfusion Service (SNBTS), Research Avenue North, Edinburgh EH14 4BE, UK., Macmillan MT; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Rodrigo-Torres D; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Dwyer BJ; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK; Curtin Medical School, Curtin Health Innovation Research Institute, Curtin University, Kent St., Bentley, Perth 6102, Australia., Gadd VL; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Ashmore-Harris C; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Lu WY; Centre for Inflammation Research (CIR), University of Edinburgh, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK., Thomson JP; Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Cancer, University of Edinburgh, Crewe Road, Edinburgh EH4 2XU, UK., Jansen MA; Centre for Cardiovascular Science, The Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK., O'Duibhir E; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Starkey Lewis PJ; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Campana L; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Aird RE; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Bate TSR; Institute or Bioengineering, School of Engineering, University of Edinburgh, Faraday Building Colin Maclaurin Road, Edinburgh EH9 3DW, UK., Fraser AR; Tissues, Cells and Advanced Therapeutics Scottish National Blood and Transfusion Service (SNBTS), Research Avenue North, Edinburgh EH14 4BE, UK., Campbell JDM; Tissues, Cells and Advanced Therapeutics Scottish National Blood and Transfusion Service (SNBTS), Research Avenue North, Edinburgh EH14 4BE, UK., Oniscu GC; Edinburgh Transplant Centre, Royal Infirmary of Edinburgh, 51 Little France Crescent, Edinburgh EH16 4SA, UK; University of Edinburgh, 51 Little France Crescent, Edinburgh EH16 4SA, UK., Hay DC; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK., Callanan A; Institute or Bioengineering, School of Engineering, University of Edinburgh, Faraday Building Colin Maclaurin Road, Edinburgh EH9 3DW, UK., Forbes SJ; Centre for Regenerative Medicine, Institute for Regeneration and Repair, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK. Electronic address: stuart.forbes@ed.ac.uk. |
| المصدر: | Cell stem cell [Cell Stem Cell] 2022 Mar 03; Vol. 29 (3), pp. 355-371.e10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101311472 Publication Model: Print Cited Medium: Internet ISSN: 1875-9777 (Electronic) Linking ISSN: 18759777 NLM ISO Abbreviation: Cell Stem Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, MA : Cell Press |
| مواضيع طبية MeSH: | Liver Transplantation*, Animals ; Bile Ducts/pathology ; Epithelial Cells/pathology ; Fibrosis ; Humans ; Living Donors ; Mice |
| مستخلص: | Biliary diseases can cause inflammation, fibrosis, bile duct destruction, and eventually liver failure. There are no curative treatments for biliary disease except for liver transplantation. New therapies are urgently required. We have therefore purified human biliary epithelial cells (hBECs) from human livers that were not used for liver transplantation. hBECs were tested as a cell therapy in a mouse model of biliary disease in which the conditional deletion of Mdm2 in cholangiocytes causes senescence, biliary strictures, and fibrosis. hBECs are expandable and phenotypically stable and help restore biliary structure and function, highlighting their regenerative capacity and a potential alternative to liver transplantation for biliary disease. Competing Interests: Declaration of interests S.J.F. and J.D.M.C. are founders and scientific advisors of Resolution Therapeutics Ltd (not related to this study). D.C.H. is a founder, director, and shareholder at Stemnovate Limited and Stimuliver ApS (not related to this study). (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.) |
| التعليقات: | Comment in: Cell Stem Cell. 2022 Mar 3;29(3):345-347. (PMID: 35245462) |
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| معلومات مُعتمدة: | MR/T029161/1 United Kingdom MRC_ Medical Research Council; MR/T030798/1 United Kingdom MRC_ Medical Research Council; MR/V03779X/1 United Kingdom MRC_ Medical Research Council; MR/K017047/1 United Kingdom MRC_ Medical Research Council; MR/P016839/1 United Kingdom MRC_ Medical Research Council; MR/T030798/2 United Kingdom MRC_ Medical Research Council; United Kingdom CRUK_ Cancer Research UK; MR/R015635/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: bile duct; biliary epithelial cells; cholangiocytes; liver; liver transplant; organoid; progenitor cells; regeneration; senescence |
| تواريخ الأحداث: | Date Created: 20220304 Date Completed: 20220418 Latest Revision: 20230322 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8900617 |
| DOI: | 10.1016/j.stem.2022.02.006 |
| PMID: | 35245467 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1875-9777 |
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| DOI: | 10.1016/j.stem.2022.02.006 |