دورية أكاديمية
أعرض في EDS

Myriocin Treatment Reverses Alcohol-Induced Alterations in Polyunsaturated Fatty Acid-Containing Phospholipid Expression in the Liver.

التفاصيل البيبلوغرافية
العنوان: Myriocin Treatment Reverses Alcohol-Induced Alterations in Polyunsaturated Fatty Acid-Containing Phospholipid Expression in the Liver.
المؤلفون: Yalcin EB; Liver Research Center, Division of Gastroenterology and Department of Medicine, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI, USA.; Division of Research, Providence VA Medical Center, Providence, RI, USA., Tong M; Liver Research Center, Division of Gastroenterology and Department of Medicine, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI, USA., Homans C; Health and Human Biology, Brown University, Providence, RI, USA., de la Monte SM; Liver Research Center, Division of Gastroenterology and Department of Medicine, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI, USA.; Division of Research, Providence VA Medical Center, Providence, RI, USA.; Department of Pathology and Laboratory Medicine, Providence VA Medical Center and the Women & Infants Hospital of Rhode Island, Providence, RI, USA.; Departments of Neurology, Neurosurgery, and Pathology, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI, USA.
المصدر: Nutrition and metabolic insights [Nutr Metab Insights] 2022 Feb 28; Vol. 15, pp. 11786388221082012. Date of Electronic Publication: 2022 Feb 28 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: SAGE Publications Country of Publication: United States NLM ID: 101550186 Publication Model: eCollection Cited Medium: Print ISSN: 1178-6388 (Print) Linking ISSN: 11786388 NLM ISO Abbreviation: Nutr Metab Insights Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: <2016- > : Thousand Oaks, CA : SAGE Publications
Original Publication: New Zealand : Libertas Academica
مستخلص: Chronic heavy alcohol exposure causes steatohepatitis manifested by abnormal intra-hepatocyte accumulation of lipid and parenchymal inflammation. Attendant alterations in polyunsaturated fatty acid (PUFA)-containing phospholipids could cause alcoholic liver disease (ALD) to progress by promoting oxidative stress, inflammation, and fibrogenesis. Previously we showed that myriocin, a serine palmitoyltransferase inhibitor, ameliorates experimental alcohol-induced steatohepatitis. However, the surprising overall therapeutic responses suggested that myriocin's targets may go beyond sphingolipids. To this end, the present study examines the effects of myriocin on hepatic composition of docosahexaenoic acid (DHA)- and arachidonic acid (AA)-containing phospholipids in an experimental model of ALD. A chronic+binge ethanol exposure model was generated by feeding Long Evans rats with ethanol-containing diets (24% caloric content) for 8 weeks and simultaneously binge gavage administering 2 g/kg ethanol on Tuesdays, Thursdays and Saturdays during Weeks 6-8. Myriocin was administered by i.p. injection on Mondays, Wednesdays, and Fridays of Weeks 3-8. Control rats were studied in parallel. Upon euthanasia, the livers were harvested to examine ethanol- and/or myriocin-modulation of hepatic lipids using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). Results were analyzed statistically by two-way analysis of variance and depicted with data bar plots and heatmaps. Chronic+binge ethanol exposures significantly increased hepatic expression of AA-containing phospholipids including PE(36:4) ( P = .005), PE(38:4) ( P = .03), and PI(38:4) ( P = .04) and reduced DHA-containing phospholipids including PS(40:6) ( P = .03) and PE(40:6) ( P = .04) relative to control. Myriocin partially reversed ethanol's effects on hepatic PUFA expression by decreasing PE(36:4) ( P = .004) and increasing PS(40:6) ( P = .04) and PI(40:6) ( P = .0003) relative to ethanol-exposed rats. Ethanol-mediated alterations in hepatic PUFA-containing phospholipids may contribute to hepatic oxidative and inflammatory injury by increasing AA and fibrogenesis by inhibiting DHA. The results suggest that Myriocin may help reduce or prevent long-term and progressive liver injury stemming from excessive chronic+binge ethanol consumption.
Competing Interests: Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(© The Author(s) 2022.)
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معلومات مُعتمدة: IK2 BX004961 United States BX BLRD VA; R01 AA011431 United States AA NIAAA NIH HHS; R01 AA028408 United States AA NIAAA NIH HHS
فهرسة مساهمة: Keywords: Alcoholic liver disease; arachidonic acid; docosahexaenoic acid; myriocin; phospholipids; rat model
تواريخ الأحداث: Date Created: 20220307 Latest Revision: 20230129
رمز التحديث: 20230129
مُعرف محوري في PubMed: PMC8891894
DOI: 10.1177/11786388221082012
PMID: 35250275
قاعدة البيانات: MEDLINE
الوصف
تدمد:1178-6388
DOI:10.1177/11786388221082012