دورية أكاديمية
Spatial distribution and community structure of microbiota associated with cowpea aphid ( Aphis craccivora Koch).
العنوان: | Spatial distribution and community structure of microbiota associated with cowpea aphid ( Aphis craccivora Koch). |
---|---|
المؤلفون: | Pawar MM; Insect Molecular Biology Laboratory, Department of Agricultural Entomology, University of Agricultural Sciences, Bangalore, 560065 India., Shivanna B; Insect Molecular Biology Laboratory, Department of Agricultural Entomology, University of Agricultural Sciences, Bangalore, 560065 India., Prasannakumar MK; Plant PathoGenOmic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, Bangalore, 560065 India., Parivallal PB; Plant PathoGenOmic Laboratory, Department of Plant Pathology, University of Agricultural Sciences, Bangalore, 560065 India., Suresh K; Department of Ecophysiology, University of Bonn, 53115 Bonn, Germany., Meenakshi NH; Insect Molecular Biology Laboratory, Department of Agricultural Entomology, University of Agricultural Sciences, Bangalore, 560065 India. |
المصدر: | 3 Biotech [3 Biotech] 2022 Mar; Vol. 12 (3), pp. 75. Date of Electronic Publication: 2022 Feb 21. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 101565857 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2190-572X (Print) Linking ISSN: 21905738 NLM ISO Abbreviation: 3 Biotech Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin : Springer |
مستخلص: | Aphid populations were collected on cowpea, dolichos, redgram and black gram from Belagavi and Udupi locations. The samples were shotgun sequenced using the Illumina NovaSeq 6000 system to understand the spatial distribution and community structure of microbiota (especially bacteria) associated with aphids. In the present study, we identified obligatory nutritional symbiont Buchnera aphidicola and facultative symbionts Rickettsia sp. and Bacteroidetes endosymbiont of Geopemphigus sp. in all the aphid samples studied, although in varied abundance. On the other hand, Serratia symbiotica , Arsenophonus sp. and Acinetobacter sp. were only found in aphids on specific host plants, suggesting that host plants might influence the bacterial community structure. Furthermore, our study revealed that microbiota other than bacteria were highly insignificant in the aphid populations. Additionally, functional annotation of aphid metagenomes identified several pathways and enzymes involved in various physiological and ecological functions. Amino acid and vitamin biosynthesis-related pathways were predominant than carbohydrate metabolism, owing to their feeding habit and nutritional requirement. Chaperones related to stress tolerance such as GroEL and DnaK were identified. Enzymes involved in toxic chemical metabolisms such as glutathione transferase, phosphodiesterases and ABC transferases were observed. These enzymes may confer resistance to pesticides in the aphid populations. Overall, our results support the importance of host plants in structuring bacterial communities in aphids and show the functional roles of symbionts in aphid survival and development. Thus, these findings can be the basis for further detailed investigations and devising better strategies to manage the pests in field conditions. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-022-03142-1. Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest in the publication. (© King Abdulaziz City for Science and Technology 2022.) |
References: | Proc Biol Sci. 2003 Dec 22;270(1533):2543-50. (PMID: 14728775) Proc Biol Sci. 2006 May 22;273(1591):1273-80. (PMID: 16720402) Microbiol Mol Biol Rev. 2004 Dec;68(4):669-85. (PMID: 15590779) Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12795-800. (PMID: 16120675) C R Biol. 2010 Jun-Jul;333(6-7):497-503. (PMID: 20541161) BMC Plant Biol. 2012 Jul 04;12:101. (PMID: 22759788) Microb Ecol. 2020 May;79(4):971-984. (PMID: 31802184) BMC Ecol. 2014 Feb 20;14:5. (PMID: 24555501) Proc Biol Sci. 2011 Mar 7;278(1706):760-6. (PMID: 20843842) Annu Rev Physiol. 1999;61:243-82. (PMID: 10099689) Insect Sci. 2017 Jun;24(3):511-521. (PMID: 26773849) Sci Rep. 2016 Apr 15;6:22958. (PMID: 27079679) ISME J. 2010 Feb;4(2):242-52. (PMID: 19907504) PLoS One. 2015 Dec 09;10(12):e0144552. (PMID: 26650541) J Biol Chem. 1998 Dec 4;273(49):32587-94. (PMID: 9829996) Appl Environ Microbiol. 2011 Dec;77(23):8345-9. (PMID: 21965398) Nucleic Acids Res. 2014 Jan;42(Database issue):D206-14. (PMID: 24293654) Appl Environ Microbiol. 2005 Jul;71(7):4069-75. (PMID: 16000822) Science. 2004 Mar 26;303(5666):1989. (PMID: 15044797) Proc Biol Sci. 2008 Feb 7;275(1632):293-9. (PMID: 18029301) Bioinformatics. 2015 May 15;31(10):1674-6. (PMID: 25609793) Cell Host Microbe. 2011 Oct 20;10(4):359-67. (PMID: 22018236) PeerJ. 2018 May 17;6:e4793. (PMID: 29785353) Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) J Exp Bot. 2006;57(4):747-54. (PMID: 16449374) Microb Ecol. 2014 Jan;67(1):195-204. (PMID: 24233285) Genome Biol. 2005;6(8):229. (PMID: 16086859) Appl Environ Microbiol. 2019 Nov 14;85(23):. (PMID: 31540983) PLoS One. 2011;6(8):e23170. (PMID: 21858016) Genetika. 2015 May;51(5):519-28. (PMID: 26137633) BMC Genomics. 2015 Mar 21;16:226. (PMID: 25887812) J Evol Biol. 2011 Apr;24(4):761-71. (PMID: 21261774) Nucleic Acids Res. 1999 Oct 1;27(19):3911-20. (PMID: 10481031) Biol Lett. 2010 Feb 23;6(1):109-11. (PMID: 19776066) Genome Biol Evol. 2011;3:195-208. (PMID: 21266540) Sci Rep. 2021 Mar 4;11(1):5260. (PMID: 33664278) Proc Biol Sci. 2012 May 7;279(1734):1791-6. (PMID: 22113034) J Insect Physiol. 2009 Oct;55(10):919-26. (PMID: 19545573) Trends Plant Sci. 2012 Aug;17(8):478-86. (PMID: 22564542) J Evol Biol. 2020 Oct;33(10):1507-1511. (PMID: 32894786) J Invertebr Pathol. 2009 Feb;100(2):123-6. (PMID: 19026656) Nucleic Acids Res. 2010 Jul;38(12):e132. (PMID: 20403810) Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286) Annu Rev Entomol. 2010;55:247-66. (PMID: 19728837) J Biochem. 1997 Jul;122(1):41-8. (PMID: 9276669) Proc Biol Sci. 2006 Mar 7;273(1586):603-10. (PMID: 16537132) Nat Methods. 2015 Jan;12(1):59-60. (PMID: 25402007) Annu Rev Genet. 2008;42:165-90. (PMID: 18983256) Evolution. 2012 Feb;66(2):375-90. (PMID: 22276535) Bull Entomol Res. 2007 Aug;97(4):407-13. (PMID: 17645822) Genome Res. 2007 Mar;17(3):377-86. (PMID: 17255551) J Econ Entomol. 2017 Apr 1;110(2):667-677. (PMID: 28334260) Nat Protoc. 2020 Mar;15(3):799-821. (PMID: 31942082) ISME J. 2018 Mar;12(3):898-908. (PMID: 29362506) Science. 2005 Dec 16;310(5755):1781. (PMID: 16357252) Trends Ecol Evol. 2000 Aug;15(8):321-326. (PMID: 10884696) J Bacteriol. 2004 Oct;186(19):6626-33. (PMID: 15375144) Science. 2009 Aug 21;325(5943):992-4. (PMID: 19696350) Mol Ecol. 2021 Jun;30(11):2483-2494. (PMID: 33756029) Front Microbiol. 2019 Apr 15;10:764. (PMID: 31037067) Appl Environ Microbiol. 2016 Apr 04;82(8):2336-2346. (PMID: 26850304) Front Physiol. 2019 Apr 17;10:438. (PMID: 31057424) J Inorg Biochem. 2014 Feb;131:1-7. (PMID: 24239906) Nucleic Acids Res. 2017 Jul 3;45(W1):W180-W188. (PMID: 28449106) Oecologia. 2016 Mar;180(3):735-47. (PMID: 26603858) Microb Ecol. 2017 Jan;73(1):201-210. (PMID: 27872949) |
فهرسة مساهمة: | Keywords: Aphids; Bacterial communities; Host plants; Metagenomics; Microbial symbionts |
تواريخ الأحداث: | Date Created: 20220307 Latest Revision: 20230302 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC8861231 |
DOI: | 10.1007/s13205-022-03142-1 |
PMID: | 35251878 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2190-572X |
---|---|
DOI: | 10.1007/s13205-022-03142-1 |