دورية أكاديمية
Evaluation of the PLAC8 Gene in Mexican Women With and Without Preeclampsia and Obesity.
| العنوان: | Evaluation of the PLAC8 Gene in Mexican Women With and Without Preeclampsia and Obesity. |
|---|---|
| المؤلفون: | Barragán-Zúñiga LJ; Laboratorio de Biología Molecular, Academia De Genómica, Instituto Politécnico Nacional-Centro Interdisciplinario de Investigacira el Desarrollo Integral Regional Durango, Durango, Mexico.; Red Iberoamericana de Alteraciones Vasculares en Trastornos del Embarazo, Chillán, Chile., Marchat LA; Laboratorio de Biomedicina Molecular II, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Mexico City, Mexico., Carrasco-Wong I; Cellular Signaling and Differentiation Laboratory, School of Medical Technology, Health Sciences Faculty, Universidad San Sebastian, Santiago, Chile., Blanco-Castaneda R; Laboratorio de Biología Molecular, Academia De Genómica, Instituto Politécnico Nacional-Centro Interdisciplinario de Investigacira el Desarrollo Integral Regional Durango, Durango, Mexico.; Red Iberoamericana de Alteraciones Vasculares en Trastornos del Embarazo, Chillán, Chile., Salas-Pacheco JM; Laboratorio de Biología Molecular, Instituto de Investigación Científica, Universidad Juárez del Estado de Durango, Durango, Mexico., Simental-Mendia LE; Biomedical Research Unit, Mexican Social Security Institute, Durango, Mexico., Correa-Ramírez MM; Laboratorio de Biología Molecular, Academia de Entomología, Instituto Politécnico Nacional-Centro Interdisciplinario de Investigacira el Desarrollo Integral Regional Durango, Durango, Mexico., Sosa-Macías M; Laboratorio de Biología Molecular, Academia De Genómica, Instituto Politécnico Nacional-Centro Interdisciplinario de Investigacira el Desarrollo Integral Regional Durango, Durango, Mexico.; Red Iberoamericana de Alteraciones Vasculares en Trastornos del Embarazo, Chillán, Chile., Gutiérrez J; Cellular Signaling and Differentiation Laboratory, School of Medical Technology, Health Sciences Faculty, Universidad San Sebastian, Santiago, Chile., Galaviz-Hernandez C; Laboratorio de Biología Molecular, Academia De Genómica, Instituto Politécnico Nacional-Centro Interdisciplinario de Investigacira el Desarrollo Integral Regional Durango, Durango, Mexico.; Red Iberoamericana de Alteraciones Vasculares en Trastornos del Embarazo, Chillán, Chile. |
| المصدر: | Frontiers in medicine [Front Med (Lausanne)] 2022 Feb 17; Vol. 9, pp. 795309. Date of Electronic Publication: 2022 Feb 17 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101648047 Publication Model: eCollection Cited Medium: Print ISSN: 2296-858X (Print) Linking ISSN: 2296858X NLM ISO Abbreviation: Front Med (Lausanne) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne, Switzerland : Frontiers Media S.A., [2014]- |
| مستخلص: | Preeclampsia (PE) is a leading cause of maternal-fetal mortality worldwide, and obesity is an important risk factor. Genes associated with pathophysiological events common to preeclampsia and obesity, such as PLAC8 , remain to be studied; therefore, the aim of the present study was to evaluate this gene in the placentas of women affected with preeclampsia and healthy pregnant women. This case-controlled study included 71 healthy and 64 preeclampsia pregnancies. Gene expression was evaluated in primary human cytotrophoblasts (PHCT) from six normal and six preeclampsia pregnancies, and protein expression was verified in placentas from five healthy and six preeclampsia pregnancies. The whole coding and 5' regions of the PLAC8 gene were sequenced from healthy ( n = 10) and preeclamptic ( n = 10) pregnancies. The presence of the observed nucleotide variations was analyzed by RT-PCR in the total population. Statistical analyses were performed accordingly. Obesity was associated with severe preeclampsia (SPE) (OR = 3.34; CI 95% 1.3-8.2, p < 0.01). Significantly higher mRNA and protein expression was observed in preeclamptic vs. healthy placentas ( p < 0.05). After sequencing, a single nucleotide variation was identified in 10 cases and one control ( p < 0.01), which was then evaluated in the total population showing no association with preeclampsia. This preliminary study confirms the association of SPE with obesity and suggests higher expression of PLAC8 mRNA and protein in placentas from preeclampsia. No differences in nucleotide variations between cases and controls of the whole population were observed. Further research is required to evaluate the implications of higher gene/protein expression in preeclampsia and the causes of such variation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Barragán-Zúñiga, Marchat, Carrasco-Wong, Blanco-Castaneda, Salas-Pacheco, Simental-Mendia, Correa-Ramírez, Sosa-Macías, Gutiérrez and Galaviz-Hernandez.) |
| References: | Ann Epidemiol. 2005 Aug;15(7):475-82. (PMID: 16029839) J Pregnancy. 2011;2011:251470. (PMID: 22132335) J Obstet Gynaecol Res. 2013 Mar;39(3):627-31. (PMID: 23107382) Malays J Med Sci. 2009 Jan;16(1):7-15. (PMID: 22589643) Hypertens Pregnancy. 2014 Feb;33(1):15-30. (PMID: 24205968) Taiwan J Obstet Gynecol. 2017 Oct;56(5):593-598. (PMID: 29037542) Reproduction. 2010 Sep;140(3):365-71. (PMID: 20457594) Med Sci Monit. 2003 Feb;9(2):RA55-61. (PMID: 12601307) J Obstet Gynaecol India. 2019 Jun;69(3):197-202. (PMID: 31178633) Placenta. 2002 May;23(5):359-72. (PMID: 12061851) Development. 2011 Jul;138(14):2987-98. (PMID: 21693515) Eur J Obstet Gynecol Reprod Biol. 2004 Jan 15;112(1):9-15. (PMID: 14687732) Lancet. 2001 Jan 6;357(9249):53-6. (PMID: 11197372) BMC Res Notes. 2012 Jun 19;5:308. (PMID: 22713493) Gene. 2003 May 8;309(2):81-9. (PMID: 12758124) BMC Syst Biol. 2015;9 Suppl 2:S4. (PMID: 25879409) Gynecol Endocrinol. 2006 Sep;22(9):521-5. (PMID: 17071538) Cell Metab. 2011 Nov 2;14(5):658-70. (PMID: 21982742) Development. 2018 Jan 29;145(2):. (PMID: 29361555) Nucleic Acids Res. 1988 Feb 11;16(3):1215. (PMID: 3344216) Am J Physiol Regul Integr Comp Physiol. 2015 Dec 1;309(11):R1326-43. (PMID: 26447211) Exp Ther Med. 2016 Oct;12(4):2634-2638. (PMID: 27698766) BJOG. 2010 Jul;117(8):997-1004. (PMID: 20482533) Front Physiol. 2018 Dec 19;9:1838. (PMID: 30618843) JAMA. 2003 May 21;289(19):2560-72. (PMID: 12748199) Endocr J. 2008 Jul;55(3):565-73. (PMID: 18490835) Syst Biol Reprod Med. 2015 Apr;61(2):65-71. (PMID: 25475254) Sci Rep. 2020 Mar 24;10(1):5264. (PMID: 32210256) Pregnancy Hypertens. 2020 Apr;20:92-95. (PMID: 32203727) Obstet Gynecol. 2013 Nov;122(5):1122-1131. (PMID: 24150027) Genet Mol Res. 2014 Sep 26;13(3):7869-78. (PMID: 25299101) Horm Mol Biol Clin Investig. 2015 Oct;24(1):47-63. (PMID: 26509784) Physiol Genomics. 2006 Dec 13;28(1):84-96. (PMID: 17018689) Trends Endocrinol Metab. 2007 Dec;18(10):365-70. (PMID: 18023357) Endocrinology. 1998 Mar;139(3):1013-22. (PMID: 9492033) J Matern Fetal Neonatal Med. 2007 Jul;20(7):509-13. (PMID: 17674263) PLoS One. 2013;8(3):e58168. (PMID: 23472151) |
| فهرسة مساهمة: | Keywords: PLAC8; gene expression; nucleotide variations; obesity; placenta; preeclampsia; protein expression |
| تواريخ الأحداث: | Date Created: 20220307 Latest Revision: 20220309 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8893357 |
| DOI: | 10.3389/fmed.2022.795309 |
| PMID: | 35252239 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2296-858X |
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| DOI: | 10.3389/fmed.2022.795309 |