دورية أكاديمية
Screening of Novel Bioactive Peptides from Goat Casein: In Silico to In Vitro Validation.
| العنوان: | Screening of Novel Bioactive Peptides from Goat Casein: In Silico to In Vitro Validation. |
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| المؤلفون: | Coscueta ER; Centro de Biotecnologia e Química Fina (CBQF)-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal., Batista P; Centro de Biotecnologia e Química Fina (CBQF)-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.; Human Neurobehavioral Laboratory/Research Centre for Human Development (HNL/CEDH), Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal., Gomes JEG; Instituto de Biociências, Letras e Ciências Exatas (IBILCE)-Rua Cristovão Colombo, Campus São José do Rio Preto, Universidade Estadual Paulista (UNESP), 2265, Jardim Nazareth, São José do Rio Preto 15054-000, SP, Brazil.; Laboratório de Microbiologia, Tecnologia Enzimática e Bioprodutos-Universidade Federal do Agreste de Pernambuco (UFAPE), Avenida Bom Pastor s/n, Boa Vista, Garanhuns 55292-270, PE, Brazil., da Silva R; Instituto de Biociências, Letras e Ciências Exatas (IBILCE)-Rua Cristovão Colombo, Campus São José do Rio Preto, Universidade Estadual Paulista (UNESP), 2265, Jardim Nazareth, São José do Rio Preto 15054-000, SP, Brazil., Pintado MM; Centro de Biotecnologia e Química Fina (CBQF)-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2022 Feb 23; Vol. 23 (5). Date of Electronic Publication: 2022 Feb 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Caseins/*chemistry , Computational Biology/*methods , Peptides/*genetics , Peptides/*pharmacology, Amino Acid Sequence ; Angiotensin-Converting Enzyme Inhibitors/pharmacology ; Animals ; Antihypertensive Agents/pharmacology ; Antioxidants/pharmacology ; Drug Evaluation, Preclinical ; Goats ; Humans ; Hydrolysis ; Software |
| مستخلص: | Food-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential. |
| References: | Agric Biol Chem. 1990 Apr;54(4):1101-2. (PMID: 1368548) Amino Acids. 2013 Mar;44(3):797-820. (PMID: 22968663) Bioorg Med Chem. 2018 Jun 1;26(10):2700-2707. (PMID: 28720325) Int J Mol Sci. 2019 Nov 27;20(23):. (PMID: 31783634) Crit Rev Food Sci Nutr. 2018 Jan 2;58(1):105-115. (PMID: 26852912) J AOAC Int. 2008 Jul-Aug;91(4):965-80. (PMID: 18727559) Sci Rep. 2015 Jul 27;5:12512. (PMID: 26213115) Peptides. 2011 May;32(5):1013-9. (PMID: 21335046) Bioinformatics. 2019 Aug 15;35(16):2757-2765. (PMID: 30590410) Food Res Int. 2014 Oct;64:241-247. (PMID: 30011646) Food Chem X. 2019 Feb 18;1:100006. (PMID: 31432006) PLoS One. 2012;7(10):e45012. (PMID: 23056189) Crit Rev Food Sci Nutr. 2021 Jan 28;:1-14. (PMID: 33506720) Med Res Rev. 2020 Jul;40(4):1276-1314. (PMID: 31922268) |
| معلومات مُعتمدة: | 2017/16482-5 São Paulo Research Foundation; 202186/2018-0 ESN National Council for Scientific and Technological Development |
| فهرسة مساهمة: | Keywords: ACE inhibition; ORAC; antihypertensive activity; antioxidant activity; bioactive peptide; bioinformatics; in silico prediction |
| المشرفين على المادة: | 0 (Angiotensin-Converting Enzyme Inhibitors) 0 (Antihypertensive Agents) 0 (Antioxidants) 0 (Caseins) 0 (Peptides) |
| تواريخ الأحداث: | Date Created: 20220310 Date Completed: 20220329 Latest Revision: 20220329 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8910560 |
| DOI: | 10.3390/ijms23052439 |
| PMID: | 35269581 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms23052439 |