دورية أكاديمية
Effect of Cobalt-Chromium-Molybdenum Implant Surface Modifications on Biofilm Development of S. aureus and S. epidermidis .
| العنوان: | Effect of Cobalt-Chromium-Molybdenum Implant Surface Modifications on Biofilm Development of S. aureus and S. epidermidis . |
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| المؤلفون: | Paulitsch-Fuchs AH; Biomedical Sciences, University of Applied Sciences Carinthia, Klagenfurt, Austria.; Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria., Bödendorfer B; Biomedical Sciences, University of Applied Sciences Carinthia, Klagenfurt, Austria., Wolrab L; Biomedical Sciences, University of Applied Sciences Carinthia, Klagenfurt, Austria., Eck N; Department of Orthopaedics and Trauma, Medical University of Graz, Graz, Austria., Dyer NP; Bioinformatics Research Technology Platform, University of Warwick, Coventry, United Kingdom., Lohberger B; Department of Orthopaedics and Trauma, Medical University of Graz, Graz, Austria. |
| المصدر: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Mar 01; Vol. 12, pp. 837124. Date of Electronic Publication: 2022 Mar 01 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media SA |
| مواضيع طبية MeSH: | Methicillin-Resistant Staphylococcus aureus* , Staphylococcal Infections*, Biofilms ; Chromium/pharmacology ; Cobalt/pharmacology ; Humans ; Molybdenum/pharmacology ; Staphylococcus aureus/genetics ; Staphylococcus epidermidis |
| مستخلص: | Periprosthetic infections are an eminent factor in patient care and also having significant economic implications. The number of biofilm-infection related replacement surgeries is increasing and will continue to do so in the following decades. To reduce both the health burden of the patients and the costs to the healthcare sector, new solutions for implant materials resistant to such infections are necessary. This study researches different surface modifications of cobalt-chromium-molybdenum (CoCrMo) based implant materials and their influence on the development of biofilms. Three smooth surfaces (CoCrMo, CoCrMo TiN, and CoCrMo polished) and three rough surfaces (CoCrMo porous coated, CoCrMo cpTi, and CoCrMo TCP) are compared. The most common infectious agents in periprosthetic infections are Staphylococcus aureus and Coagulase-negative staphylococci (e.g., Staphylococcus epidermidis ), therefore strains of these two species have been chosen as model organisms. Biofilms were grown on material disks for 48 h and cell number, polysaccharide content, and protein contend of the biofilms were measured. Additionally, regulation of genes involved in early biofilm development ( S. aureus icaA , icaC , fnbA , fnbB , clfB , atl ; S. epidermidis atlE , aap ) was detected using RT-q-PCR. All results were compared to the base alloy without modifications. The results show a correlation between the surface roughness and the protein and polysaccharide content of biofilm structures and also the gene expression of the biofilms grown on the different surface modifications. This is supported by the significantly different protein and polysaccharide contents of the biofilms associated with rough and smooth surface types. Additionally, early phase biofilm genes (particularly icaA , icaC , and aap ) are statistically significantly downregulated compared to the control at 48 h on rough surfaces. CoCrMo TiN and polished CoCrMo were the two smooth surface modifications which performed best on the basis of low biofilm content. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Paulitsch-Fuchs, Bödendorfer, Wolrab, Eck, Dyer and Lohberger.) |
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| فهرسة مساهمة: | Keywords: CoCrMo; Staphylococcus aureus; Staphylococcus epidermidis; biofilms; prosthetic infections |
| المشرفين على المادة: | 0R0008Q3JB (Chromium) 3G0H8C9362 (Cobalt) 81AH48963U (Molybdenum) |
| تواريخ الأحداث: | Date Created: 20220318 Date Completed: 20220406 Latest Revision: 20220406 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8921486 |
| DOI: | 10.3389/fcimb.2022.837124 |
| PMID: | 35300379 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2235-2988 |
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| DOI: | 10.3389/fcimb.2022.837124 |