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Expression of Circ_Satb1 Is Decreased in Mesial Temporal Lobe Epilepsy and Regulates Dendritic Spine Morphology.

التفاصيل البيبلوغرافية
العنوان: Expression of Circ_Satb1 Is Decreased in Mesial Temporal Lobe Epilepsy and Regulates Dendritic Spine Morphology.
المؤلفون: Gomes-Duarte A; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Venø MT; Interdisciplinary Nanoscience Center, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.; Omiics ApS, Aarhus, Denmark., de Wit M; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Senthilkumar K; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Broekhoven MH; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., van den Herik J; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Heeres FR; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., van Rossum D; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Rybiczka-Tesulov M; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Legnini I; Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany., van Rijen PC; Department of Neurology and Neurosurgery, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., van Eijsden P; Department of Neurology and Neurosurgery, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Gosselaar PH; Department of Neurology and Neurosurgery, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Rajewsky N; Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany., Kjems J; Interdisciplinary Nanoscience Center, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark., Vangoor VR; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Pasterkamp RJ; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands.
المصدر: Frontiers in molecular neuroscience [Front Mol Neurosci] 2022 Mar 03; Vol. 15, pp. 832133. Date of Electronic Publication: 2022 Mar 03 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101477914 Publication Model: eCollection Cited Medium: Print ISSN: 1662-5099 (Print) Linking ISSN: 16625099 NLM ISO Abbreviation: Front Mol Neurosci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne, Switzerland : Frontiers Research Foundation, 2008-
مستخلص: Mesial temporal lobe epilepsy (mTLE) is a chronic disease characterized by recurrent seizures that originate in the temporal lobes of the brain. Anti-epileptic drugs (AEDs) are the standard treatment for managing seizures in mTLE patients, but are frequently ineffective. Resective surgery is an option for some patients, but does not guarantee a postoperative seizure-free period. Therefore, further insight is needed into the pathogenesis of mTLE to enable the design of new therapeutic strategies. Circular RNAs (circRNAs) have been identified as important regulators of neuronal function and have been implicated in epilepsy. However, the mechanisms through which circRNAs contribute to epileptogenesis remain unknown. Here, we determine the circRNA transcriptome of the hippocampus and cortex of mTLE patients by using RNA-seq. We report 333 differentially expressed (DE) circRNAs between healthy individuals and mTLE patients, of which 23 circRNAs displayed significant adjusted p -values following multiple testing correction. Interestingly, hippocampal expression of circ_Satb1, a circRNA derived from special AT-rich sequence binding protein 1 ( SATB1 ), is decreased in both mTLE patients and in experimental epilepsy. Our work shows that circ_Satb1 displays dynamic patterns of neuronal expression in vitro and in vivo . Further, circ_Satb1-specific knockdown using CRISPR/CasRx approaches in hippocampal cultures leads to defects in dendritic spine morphology, a cellular hallmark of mTLE. Overall, our results identify a novel epilepsy-associated circRNA with disease-specific expression and previously unidentified cellular effects that are relevant for epileptogenesis.
Competing Interests: MTV was employed by the company Omiics ApS. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Gomes-Duarte, Venø, de Wit, Senthilkumar, Broekhoven, van den Herik, Heeres, van Rossum, Rybiczka-Tesulov, Legnini, van Rijen, van Eijsden, Gosselaar, Rajewsky, Kjems, Vangoor and Pasterkamp.)
References: Brain. 2015 Apr;138(Pt 4):891-901. (PMID: 25681415)
J Neurosci. 2019 Jun 26;39(26):5064-5079. (PMID: 31015341)
PLoS One. 2011;6(10):e26932. (PMID: 22046416)
J Nanobiotechnology. 2020 Jul 25;18(1):105. (PMID: 32711535)
Electroencephalogr Clin Neurophysiol. 1972 Mar;32(3):281-94. (PMID: 4110397)
Pediatr Neurol. 1995 Apr;12(3):201-6. (PMID: 7619185)
Proteomics. 2009 Jun;9(12):3303-15. (PMID: 19562802)
Epilepsia. 1996;37 Suppl 1:S60-5. (PMID: 8647053)
RNA Biol. 2016;13(1):34-42. (PMID: 26669964)
Arch Dis Child Fetal Neonatal Ed. 1998 Mar;78(2):F116-20. (PMID: 9577281)
Mol Cell. 2014 Oct 2;56(1):55-66. (PMID: 25242144)
Cancer Res. 2004 Aug 1;64(15):5245-50. (PMID: 15289330)
Front Mol Neurosci. 2019 Feb 12;12:33. (PMID: 30809123)
J Clin Invest. 2019 Apr 30;129(7):2946-2951. (PMID: 31039137)
Lancet. 2006 Apr 1;367(9516):1087-1100. (PMID: 16581409)
PLoS One. 2012;7(2):e30733. (PMID: 22319583)
Sci Rep. 2016 Jul 22;6:30298. (PMID: 27444269)
Brain. 2015 May;138(Pt 5):1208-22. (PMID: 25765328)
Nat Med. 2012 Jul;18(7):1087-94. (PMID: 22683779)
Genome Biol. 2009;10(3):R25. (PMID: 19261174)
Neuroscience. 2013 Oct 22;251:141-50. (PMID: 22522469)
Nat Neurosci. 2015 Apr;18(4):603-610. (PMID: 25714049)
BMC Bioinformatics. 2009 Feb 03;10:48. (PMID: 19192299)
N Engl J Med. 1996 Mar 7;334(10):647-52. (PMID: 8592530)
Mol Psychiatry. 2020 Nov;25(11):2712-2727. (PMID: 31988434)
Arch Phys Med Rehabil. 2003 Mar;84(3):365-73. (PMID: 12638104)
J Neurophysiol. 1995 Apr;73(4):1706-11. (PMID: 7643176)
Biomed Pharmacother. 2020 Aug;128:110251. (PMID: 32480219)
J Neurosci. 2018 Jan 17;38(3):613-630. (PMID: 29196317)
J Biomed Biotechnol. 2001;1(1):18-24. (PMID: 12488622)
J Neurosci. 2012 Dec 5;32(49):17690-705. (PMID: 23223290)
Mol Cell Biol. 2012 Jan;32(2):333-47. (PMID: 22064485)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Cell Death Dis. 2021 May 11;12(5):468. (PMID: 33976116)
Cell. 2014 Sep 25;159(1):134-147. (PMID: 25242744)
Science. 1993 Apr 9;260(5105):181-6. (PMID: 8097060)
J Neurol Neurosurg Psychiatry. 2017 Nov;88(11):933-940. (PMID: 28870986)
J Neurophysiol. 1987 Mar;57(3):869-88. (PMID: 3031235)
Neuron. 2017 Sep 27;96(1):43-55. (PMID: 28957675)
Brain. 2012 May;135(Pt 5):1348-69. (PMID: 22427329)
Cell Physiol Biochem. 2018;45(2):677-691. (PMID: 29428937)
Cell Discov. 2018 Sep 18;4:48. (PMID: 30245844)
Ann Neurol. 1996 Aug;40(2):227-35. (PMID: 8773604)
Neurosci Lett. 1986 Aug 29;69(2):156-61. (PMID: 3763042)
Hippocampus. 2000;10(5):617-25. (PMID: 11075833)
PLoS Genet. 2013;9(9):e1003777. (PMID: 24039610)
Lancet Neurol. 2016 Dec;15(13):1368-1376. (PMID: 27839653)
J Neurosci. 2012 May 23;32(21):7267-77. (PMID: 22623672)
Neuron. 2014 Oct 1;84(1):63-77. (PMID: 25242217)
J Neurosci Methods. 2006 Sep 30;156(1-2):293-304. (PMID: 16569437)
J Gen Physiol. 2020 Mar 2;152(3):. (PMID: 31995133)
Epilepsia. 2013 May;54(5):840-7. (PMID: 23551133)
RNA. 2014 Dec;20(12):1829-42. (PMID: 25404635)
Curr Opin Neurol. 2001 Apr;14(2):187-92. (PMID: 11262734)
Cell Mol Life Sci. 2015 Oct;72(19):3621-35. (PMID: 26047658)
Genome Res. 2005 Jan;15(1):137-45. (PMID: 15590943)
Front Genet. 2020 Sep 25;11:564301. (PMID: 33101384)
Brain Pathol. 2012 May;22(3):350-79. (PMID: 22497610)
Prog Neurobiol. 2013 Jul-Aug;106-107:1-16. (PMID: 23583307)
Nucleic Acids Res. 2006 May 08;34(8):e63. (PMID: 16682442)
Mol Cell. 2015 Jun 4;58(5):870-85. (PMID: 25921068)
Sci Rep. 2016 Dec 13;6:38907. (PMID: 27958329)
Epilepsia. 1996 Aug;37(8):701-8. (PMID: 8764806)
Ann Neurol. 1993 Dec;34(6):774-80. (PMID: 8250525)
Genome Biol. 2015 Nov 05;16:245. (PMID: 26541409)
Nature. 2013 Mar 21;495(7441):333-8. (PMID: 23446348)
PLoS One. 2018 Dec 28;13(12):e0209829. (PMID: 30592747)
Seizure. 2017 Jan;44:11-20. (PMID: 28007376)
Int J Mol Sci. 2019 Oct 02;20(19):. (PMID: 31581735)
Cell Mol Life Sci. 2012 Sep;69(18):3127-45. (PMID: 22535415)
PLoS One. 2016 Jul 14;11(7):e0159464. (PMID: 27415827)
Epilepsia. 2013 Jul;54(7):1315-29. (PMID: 23692496)
Front Genet. 2021 Jan 28;12:627907. (PMID: 33584828)
PLoS One. 2015 Oct 23;10(10):e0141298. (PMID: 26496077)
Epilepsy Res Treat. 2012;2012:624519. (PMID: 22957233)
Nat Rev Dis Primers. 2018 May 03;4:18024. (PMID: 29722352)
RNA. 2013 Feb;19(2):141-57. (PMID: 23249747)
Nat Neurosci. 2001 Oct;4(10):1006-13. (PMID: 11574832)
Nat Biotechnol. 2020 Jun;38(6):722-727. (PMID: 32518401)
Nucleic Acids Res. 2009 Jan;37(Database issue):D846-51. (PMID: 18984621)
Pharmaceuticals (Basel). 2018 Feb 07;11(1):. (PMID: 29414852)
J Clin Neurophysiol. 2012 Oct;29(5):356-65. (PMID: 23027091)
Neurology. 1999 Jul 22;53(2):440. (PMID: 10430456)
Epilepsy Behav. 2005 Dec;7(4):569-77. (PMID: 16246628)
Epilepsy Behav. 2014 Aug;37:59-70. (PMID: 24980390)
Curr Opin Neurobiol. 2017 Aug;45:1-8. (PMID: 28219682)
Neurol Clin. 2016 Nov;34(4):837-847. (PMID: 27719996)
Nat Rev Genet. 2019 Nov;20(11):675-691. (PMID: 31395983)
Continuum (Minneap Minn). 2013 Jun;19(3 Epilepsy):730-42. (PMID: 23739107)
Cell Physiol Biochem. 2018;51(3):1399-1409. (PMID: 30485839)
Cold Spring Harb Perspect Med. 2015 Oct 05;5(12):. (PMID: 26438606)
Cell. 2018 Apr 19;173(3):665-676.e14. (PMID: 29551272)
Neuron. 2008 Feb 7;57(3):364-77. (PMID: 18255030)
Genome Biol. 2007;8(3):R32. (PMID: 17343748)
J Neurosci. 1996 May 1;16(9):2983-94. (PMID: 8622128)
Front Mol Neurosci. 2017 Aug 10;10:249. (PMID: 28848386)
فهرسة مساهمة: Keywords: RNA-sequencing; circular RNA; dendritic spine; epilepsy; hippocampus
تواريخ الأحداث: Date Created: 20220321 Latest Revision: 20220322
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8927295
DOI: 10.3389/fnmol.2022.832133
PMID: 35310884
قاعدة البيانات: MEDLINE
الوصف
تدمد:1662-5099
DOI:10.3389/fnmol.2022.832133