دورية أكاديمية

Broad thermal spectrum metagenomic laccase with action for dye decolorization and fentin hydroxide treatment.

التفاصيل البيبلوغرافية
العنوان: Broad thermal spectrum metagenomic laccase with action for dye decolorization and fentin hydroxide treatment.
المؤلفون: Lima NSM; Department of Agricultural and Environmental Biotechnology, São Paulo State University, Jaboticabal Campus, Jaboticabal, São Paulo State, 14884-900, Brazil.; Molecular Biology Laboratory, Institute for Research in Bioenergy (IPBEN), Jaboticabal, São Paulo State, 14884-900, Brazil.; Graduate Program in Agricultural and Livestock Microbiology, School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil., Gomes-Pepe ES; Department of Agricultural and Environmental Biotechnology, São Paulo State University, Jaboticabal Campus, Jaboticabal, São Paulo State, 14884-900, Brazil.; Molecular Biology Laboratory, Institute for Research in Bioenergy (IPBEN), Jaboticabal, São Paulo State, 14884-900, Brazil., Campanharo JC; Department of Agricultural and Environmental Biotechnology, São Paulo State University, Jaboticabal Campus, Jaboticabal, São Paulo State, 14884-900, Brazil., de Macedo Lemos EG; Department of Agricultural and Environmental Biotechnology, São Paulo State University, Jaboticabal Campus, Jaboticabal, São Paulo State, 14884-900, Brazil. eliana.lemos@unesp.br.; Molecular Biology Laboratory, Institute for Research in Bioenergy (IPBEN), Jaboticabal, São Paulo State, 14884-900, Brazil. eliana.lemos@unesp.br.
المصدر: AMB Express [AMB Express] 2022 Mar 23; Vol. 12 (1), pp. 38. Date of Electronic Publication: 2022 Mar 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101561785 Publication Model: Electronic Cited Medium: Print ISSN: 2191-0855 (Print) Linking ISSN: 21910855 NLM ISO Abbreviation: AMB Express Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Heidelberg : Springer-Verlag, c2011-
مستخلص: Laccases are multicopper oxidases that act on various phenolic and non-phenolic compounds, enabling numerous applications including xenobiotic bioremediation, biofuel production, drug development, and cosmetic production, and they can be used as additives in the textile and food industries. This wide range of uses makes these enzymes extremely attractive for novel biotechnology applications. Here, we undertook the kinetic characterization of LacMeta, a predicted as homotrimeric (~ 107,93 kDa) small laccase, and demonstrated that this enzyme performs best at an acidic pH (pH 3-5) towards ABTS as substrate and has a broad thermal spectrum (10-60 °C), which can promote high plastic action potential through dynamic environmental temperature fluctuations. This enzyme showed following kinetic parameters: k cat  = 6.377 s -1  ± 0.303, K m  = 4.219 mM, and V max  = 24.43 µM/min (against ABTS as substrate). LacMeta almost completely degraded malachite green (50 mg/mL) in only 2 h. Moreover, the enzyme was able to degrade seven dyes from four distinct classes and it respectively achieved 85% and 83% decolorization of methylene blue and trypan blue with ABTS as the mediator. In addition, LacMeta showed potential for the degradation of two thirds of an agricultural fungicide: fentin hydroxide, thus demonstrating its biotechnological aptitude for bioremediation. The results of this study suggest that LacMeta has potential in textile wastewater treatment and that it could help in the bioremediation of other human/environmental toxins such as pesticides and antibiotic compounds belonging to the same chemical classes as the degraded dyes.
(© 2022. The Author(s).)
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معلومات مُعتمدة: 2011/10981-3 FAPESP
فهرسة مساهمة: Keywords: Bioremediation; Dyes; Laccase; Metagenomics
تواريخ الأحداث: Date Created: 20220324 Latest Revision: 20220409
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8943092
DOI: 10.1186/s13568-022-01375-0
PMID: 35322306
قاعدة البيانات: MEDLINE
الوصف
تدمد:2191-0855
DOI:10.1186/s13568-022-01375-0