دورية أكاديمية
Pharmacological investigation of antioxidant and anti-inflammatory activities of aqueous extract from Mitracarpus frigidus (Rubiaceae).
| العنوان: | Pharmacological investigation of antioxidant and anti-inflammatory activities of aqueous extract from Mitracarpus frigidus (Rubiaceae). |
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| المؤلفون: | Lemos ASO; Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.; Postgraduate Program in Biological Sciences, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Campos LM; Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Souza TF; Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Paula PL; Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Granato JDT; Department of Parasitology, Microbiology, and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.; Postgraduate Program in Biological Sciences, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., da Silva JVG; Postgraduate Program in Biological Sciences, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Aragão DMO; Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Rocha VN; Department of Veterinary Medicine, Faculty of Medicine, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Coimbra ES; Department of Parasitology, Microbiology, and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.; Postgraduate Program in Biological Sciences, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Fabri RL; Bioactive Natural Products Laboratory, Department of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.; Postgraduate Program in Biological Sciences, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil. |
| المصدر: | The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2022 May 20; Vol. 74 (5), pp. 750-760. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0376363 Publication Model: Print Cited Medium: Internet ISSN: 2042-7158 (Electronic) Linking ISSN: 00223573 NLM ISO Abbreviation: J Pharm Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: London : Pharmaceutical Society of Great Britain |
| مواضيع طبية MeSH: | Rubiaceae*/chemistry, Anti-Inflammatory Agents/therapeutic use ; Antioxidants/chemistry ; Edema/chemically induced ; Edema/drug therapy ; Humans ; Phenols/pharmacology ; Plant Extracts/chemistry ; Quercetin |
| مستخلص: | Objectives: This study aimed to evaluate the potential of aqueous extract from Mitracarpus frigidus aerial parts (MFAq) in the treatment of inflammation and oxidative stress, as well as to characterize its chemical constituents. Methods: Total phenolic and flavonoid contents were determined, and phytoconstituents were detected by ultra-fast liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry (UFLC-QTOF-MS). The antioxidant activity was evaluated by DPPH, TAC and β-carotene/linoleic acid assays. In-vitro anti-inflammatory activity, cell viability and cell cycle were performed in J774A.1 cell line. In-vivo anti-inflammatory activity was investigated by two ear oedema assays (croton oil and phenol). Key Findings: Chlorogenic acid, clarinoside, quercetin-hexosylpentoside, rutin, kaempferol-3-O-rutinoside, kaempferol-rhamnosylhexoside, quercetin-pentosylrhamnosylhexoside, harounoside, 2-azaanthraquinone and sucrose were identified by UFLC-QTOF-MS. MFAq showed antioxidant activity, which was positively correlated to the content of phenolic compounds. MFAq significantly inhibited the production of nitric oxide, did not decrease viability in MTT assay (all concentrations) and showed no changes in membrane permeability and cell cycle of J774A.1 cell line. Furthermore, MFAq showed a reduction in ear oedema in all tested doses. Conclusion: MFAq was effective in some antioxidant and inflammatory parameters, in the experimental conditions that were used in the study. This is the first report of chemical composition and bioactivities from this extract. (© The Author(s) 2022. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | APQ-01059-14 Fundação de Amparo à Pesquisa do Estado de Minas Gerais |
| فهرسة مساهمة: | Keywords: Mitracarpus frigidus; inflammation; oxidative stress; phytochemistry |
| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Antioxidants) 0 (Phenols) 0 (Plant Extracts) 9IKM0I5T1E (Quercetin) |
| تواريخ الأحداث: | Date Created: 20220324 Date Completed: 20220524 Latest Revision: 20220524 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/jpp/rgac005 |
| PMID: | 35325209 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2042-7158 |
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| DOI: | 10.1093/jpp/rgac005 |