دورية أكاديمية
Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10× Genomics' Single-Cell RNA-Seq.
| العنوان: | Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10× Genomics' Single-Cell RNA-Seq. |
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| المؤلفون: | Lee DP; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore., Ray WJ; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore., Mei TP; Molecular Engineering Laboratory, Institute of Molecular and Cell Biology (IMCB), Singapore, Singapore., Hoon S; Molecular Engineering Laboratory, Institute of Molecular and Cell Biology (IMCB), Singapore, Singapore., Scolnick J; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore., Yeo GW; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. gene.w.yeo@gmail.com.; Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA. gene.w.yeo@gmail.com. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2463, pp. 67-80. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Oligonucleotides*/genetics , Proteomics*, Antibodies ; Click Chemistry/methods ; RNA-Seq |
| مستخلص: | Recent advances in multimodal approaches toward single-cell analyses present valuable data points that can complement standard flow cytometry data. In particular, the overlay of cell-surface proteome data with gene expression analysis presents a necessary advancement, particularly in the field of immunology. Here we describe a copper-free click chemistry method for the generation of antibody-oligonucleotide complexes and present the steps for its employment in the context of the 10× genomics droplet-based single-cell RNA-seq workflow, providing a method for coupling proteomic and transcriptomic analyses in an efficient and cost-effect manner. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: 10× Genomics; Antibody conjugation; Multi-omics; SPAAC; Single-cell RNA-seq; Single-cell proteomics |
| المشرفين على المادة: | 0 (Antibodies) 0 (Oligonucleotides) |
| تواريخ الأحداث: | Date Created: 20220328 Date Completed: 20220401 Latest Revision: 20220623 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-0716-2160-8_6 |
| PMID: | 35344168 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-2160-8_6 |