دورية أكاديمية
NBS-LRR-WRKY genes and protease inhibitors (PIs) seem essential for cowpea resistance to root-knot nematode.
| العنوان: | NBS-LRR-WRKY genes and protease inhibitors (PIs) seem essential for cowpea resistance to root-knot nematode. |
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| المؤلفون: | Ribeiro DG; Centro de Análises Proteômicas e Bioquímicas Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília (UCB), Brasília, DF, Brazil., Mota APZ; Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil., Santos IR; Programa de Pós-Graduação em Ciências Biológicas (Biologia Molecular), Universidade de Brasília, Instituto de Ciências Biológicas, Campus Universitário Darcy Ribeiro - UnB, Brasília, DF, Brazil., Arraes FBM; Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil., Grynberg P; Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil., Fontes W; Laboratório de Química e Bioquímica de Proteínas, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Castro MS; Laboratório de Química e Bioquímica de Proteínas, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., de Sousa MV; Laboratório de Química e Bioquímica de Proteínas, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil., Lisei-de-Sá ME; Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil., Grossi-de-Sá MF; Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil., Franco OL; Centro de Análises Proteômicas e Bioquímicas Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília (UCB), Brasília, DF, Brazil; S-Inova Biotech, Universidade Católica Dom Bosco (UCDB), Campo Grande, MS, Brazil., Mehta A; Embrapa Recursos Genéticos e Biotecnologia, Brasilia, DF, Brazil. Electronic address: angela.mehta@embrapa.br. |
| المصدر: | Journal of proteomics [J Proteomics] 2022 Jun 15; Vol. 261, pp. 104575. Date of Electronic Publication: 2022 Mar 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Tylenchoidea*/physiology , Vigna*/genetics, Animals ; Plant Diseases ; Plant Roots/metabolism ; Protease Inhibitors/metabolism ; Proteomics |
| مستخلص: | Cowpea (Vigna unguiculata L. Walp) is a legume of great economic importance, however it is highly affected by nematodes. The present work aimed to identify proteins and genes involved in nematode resistance by proteomic and transcriptomic analysis. Plants of a genotype resistant (CE31) to root-knot nematode (Meloidogyne spp.) were collected 12 days after inoculation with Meloidogyne incognita and the total proteins and RNA were extracted from the root samples. Shotgun proteomic analysis was performed using an Orbitrap Elite mass spectrometer and the construction and sequencing of cDNA libraries were carried out in a Hi-Seq 2000 sequencing system. The proteomic and transcriptomic analyses revealed key processes involved in cowpea defense and some interesting candidates were further analyzed by RT-qPCR. Proteins and genes involved in essential biological processes were differentially accumulated such as, regulation of transcription, cell wall stiffening and microtubule-based process. However, the main defense strategies of Vigna unguiculata seem to be focused on the interaction of NBS-LRR and WRKY genes for the activation of R genes, production of protease inhibitors and maintenance of actin cytoskeleton. These are key processes that can culminate in the suppression of giant cell formation and consequently in the development of Meloidogyne incognita. SIGNIFICANCE: In this study, we identified proteins and transcripts regulated in cowpea resistant to the nematode Meloidogyne spp. upon inoculation. The results revealed key candidate genes involved in the activation of R genes, the production of protease inhibitors and maintenance of the actin cytoskeleton. These processes might be essential for cowpea resistance, as they can impede nematode nutrition, giant cell formation and consequently the development of Meloidogyne incognita. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Incompatible interaction; Meloidogyne incognita; Proteome; Transcriptome; Vigna unguiculata |
| المشرفين على المادة: | 0 (Protease Inhibitors) |
| تواريخ الأحداث: | Date Created: 20220330 Date Completed: 20220512 Latest Revision: 20220529 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jprot.2022.104575 |
| PMID: | 35351660 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1876-7737 |
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| DOI: | 10.1016/j.jprot.2022.104575 |