دورية أكاديمية
أعرض في EDS

Functionality of natural killer cells in obese asthma phenotypes.

التفاصيل البيبلوغرافية
العنوان: Functionality of natural killer cells in obese asthma phenotypes.
المؤلفون: Pur Ozyigit L; Adult Allergy Service, Glenfield Hospital, University Hospitals of Leicester NHS Trust, Leicester, UK.; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey., Aktas EC; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey., Gelmez YM; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey., Ozturk AB; Department of Allergy and Immunology, Faculty of Medicine, Koc University, Istanbul., Gemicioglu B; Department of Pulmonary Diseases, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey., Deniz G; Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
المصدر: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [Clin Exp Allergy] 2022 Dec; Vol. 52 (12), pp. 1432-1439. Date of Electronic Publication: 2022 Apr 07.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8906443 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2222 (Electronic) Linking ISSN: 09547894 NLM ISO Abbreviation: Clin Exp Allergy Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford : Blackwell Scientific Publications, c1989-
مواضيع طبية MeSH: Interleukin-17*/metabolism , Killer Cells, Natural*/metabolism, Humans ; Interferon-gamma ; Interleukin-12/metabolism ; Interleukin-12/pharmacology ; Obesity
مستخلص: Background: Obesity-associated asthma (OA) is a difficult to treat asthma phenotype due to its severity and poor response to inhaled steroids. Early-onset allergic (EoOA) and late-onset non-allergic (LoOA) OA are suggested subtypes of this phenotype. Natural Killer (NK) cells are key elements of innate immunity involved in cytotoxicity and immune regulation, with uncertain role in OA pathogenesis.
Methods: Early-onset allergic and LoOA patients together with obese non-asthmatic (ONA) controls have been enrolled in the study. Peripheral blood samples have been collected for analysis. Percentages of total NK cells, CD3 - CD56 dim and CD3 - CD56 bright NK cell subsets, cytotoxic activity, intracellular interferon-γ, interleukin (IL)-10, IL-13, IL-17 secretion and activatory receptors (NKG2D, NKp46i and NKp44) have been investigated by flow cytometry. The effect of IL-12 and IL-23 stimulation on NK cells and intracellular cytokines in different groups have also been analysed and compared with unstimulated conditions.
Results: Results of ONA (n = 5, age 42 ± 8), EoOA (n = 5, age 42 ± 10) and LoOA (n = 8, age 46 ± 8) patients have analysed. Body Mass Index has been found to be negatively correlated with CD69 (p = .022, r = -0.534). NKG2D receptor has been significantly low in CD56 dim cells of asthma population (p = .046). NKp44 receptor expression has increased after IL-12 stimulation in EoOA and control group (p = .02). Intracellular IL-10 content has increased in LoOA and control subjects (p = .018, p = .03) but not in the EoOA group. Intracellular IL-17 level has found be higher in allergic OA group. LoOA patients showed a decreased NK cytotoxicity compared with the early-onset asthma group (p = .05).
Conclusion: Our study suggests an impaired NK receptor expression, activation and reduced cytotoxicity in OA patients together with variances between different subtypes of this phenotype. This data would be beneficial for tailoring a more personalized treatment strategy combatting steroid resistance and frequent exacerbations in this group of patients.
(© 2022 John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: CD107a; CD69; NK; allergy; asthma; cytotoxicity; early-onset; obesity; phenotype
المشرفين على المادة: 0 (Interleukin-17)
82115-62-6 (Interferon-gamma)
187348-17-0 (Interleukin-12)
تواريخ الأحداث: Date Created: 20220331 Date Completed: 20221214 Latest Revision: 20230124
رمز التحديث: 20240628
DOI: 10.1111/cea.14136
PMID: 35359028
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2222
DOI:10.1111/cea.14136