دورية أكاديمية
أعرض في EDS

Epidemiology of Campylobacter infections among children of 0-24 months of age in South Africa.

التفاصيل البيبلوغرافية
العنوان: Epidemiology of Campylobacter infections among children of 0-24 months of age in South Africa.
المؤلفون: Samie A; Department of Microbiology, University of Venda, Thohoyandou, South Africa., Moropeng RC; Department of Earth, Water and Science, Tshwane University of Technology, Pretoria, South Africa., Tanih NF; Department of Medical Laboratory Sciences, University of Buea, P.O Box 63, Buea, South West Region, Cameroon. nicofriline@yahoo.com., Dillingham R; Center for Global Health, University of Virginia, Charlottesville, USA., Guerrant R; Center for Global Health, University of Virginia, Charlottesville, USA., Bessong PO; HIV/AIDS & Global Health Research Programme, University of Venda, Thohoyandou, South Africa.
المصدر: Archives of public health = Archives belges de sante publique [Arch Public Health] 2022 Apr 02; Vol. 80 (1), pp. 107. Date of Electronic Publication: 2022 Apr 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 9208826 Publication Model: Electronic Cited Medium: Print ISSN: 0778-7367 (Print) Linking ISSN: 07787367 NLM ISO Abbreviation: Arch Public Health Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: <2011-> : London : BioMed Central
Original Publication: Brussels : "Archives belges de médecine sociale" asbl., [1990-
مستخلص: Background: Campylobacter spp. are one of the most frequent causes of diarrhoeal disease in humans throughout the world. This study aimed at determining the prevalence and the genotypic distribution of Campylobacter spp. and their association with diarrhoea and child growth in children of less than the age of two in the Limpopo Province of South Africa.
Methods: A total of 4280 diarrheal and non-diarrheal stool samples were collected on a monthly basis from children recruited at birth and followed up to 24 months. All stool samples were screened for the presence Campylobacter antigen using ELISA technique after which CAH 16S primer was used on the positive samples to confirm the presence of Campylobacter. Subsequently, the PCR positive samples were further characterised using species specific primers for Campylobacter jejuni and Campylobacter coli.
Results: Campylobacter antigen was detected in 564/4280 (13.2%). Campylobacter was more commonly found in diarrheal stools (20.4%) compared to non-diarrheal stools (12.4%) with a statistically significant difference (χ 2  = 7.345; p = 0.006). Throughout the year there were two main peaks of Campylobacter infection one in December- January and the second peak in June. The prevalence of Campylobacter increased with the age of the children up to 11 months after which the prevalence decreased. Out of 564 positive ELISA samples, 257 (45.6%) were confirmed to have 16S rRNA gene for Campylobacter spp. Furthermore, C. jejuni was found to be more prevalent (232/257) than C. coli (25/257) with a prevalence of 90.3% and 9.7%, respectively. Both C. jejuni and C. coli were significantly associated with diarrhea with statistical values of (χ 2  = 22.224; p < 0.001) and (χ 2  = 81.682; p < 0.001) respectively. Sequences generated from the analysis of hip gene confirmed the PCR positives samples were C. jejuni positive.
Conclusions: This study has delineated a high prevalence of Campylobacter spp. in the study cohort. Moreover, C. jejuni was found to be more prevalent than C. coli both of which were associated with diarrhea. These findings are of clinical and epidemiological significance.
(© 2022. The Author(s).)
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فهرسة مساهمة: Keywords: Campylobacter spp.; ELISA; PCR; Prevalence; South Africa; Young Children
تواريخ الأحداث: Date Created: 20220403 Latest Revision: 20220408
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8976392
DOI: 10.1186/s13690-022-00850-1
PMID: 35366935
قاعدة البيانات: MEDLINE
الوصف
تدمد:0778-7367
DOI:10.1186/s13690-022-00850-1