دورية أكاديمية
Application of piconewton forces to individual filopodia reveals mechanosensory role of L-type Ca 2+ channels.
| العنوان: | Application of piconewton forces to individual filopodia reveals mechanosensory role of L-type Ca 2+ channels. |
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| المؤلفون: | Efremov AK; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China. Electronic address: artem@szbl.ac.cn., Yao M; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China., Sun Y; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore., Tee YH; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore., Sheetz MP; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Department of Biochemistry & Molecular Biology, The University of Texas Medical Branch, USA., Bershadsky AD; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Weizmann Institute of Science, Rehovot, 7610001, Israel., Martinac B; Victor Chang Cardiac Research Institute, Sydney, New South Wales 2010, Australia., Yan J; Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Department of Physics, National University of Singapore, Singapore 117551, Singapore. Electronic address: phyyj@nus.edu.sg. |
| المصدر: | Biomaterials [Biomaterials] 2022 May; Vol. 284, pp. 121477. Date of Electronic Publication: 2022 Mar 31. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1995-> : Amsterdam : Elsevier Science Original Publication: [Guilford, England] : IPC Science and Technology Press, 1980- |
| مواضيع طبية MeSH: | Extracellular Matrix*/metabolism , Pseudopodia*, Calcium/metabolism ; Cell Movement ; Optical Tweezers ; Signal Transduction |
| مستخلص: | Filopodia are ubiquitous membrane projections that play crucial role in guiding cell migration on rigid substrates and through extracellular matrix by utilizing yet unknown mechanosensing molecular pathways. As recent studies show that Ca 2+ channels localized to filopodia play an important role in regulation of their formation and since some Ca 2+ channels are known to be mechanosensitive, force-dependent activity of filopodial Ca 2+ channels might be linked to filopodia's mechanosensing function. We tested this hypothesis by monitoring changes in the intra-filopodial Ca 2+ level in response to application of stretching force to individual filopodia of several cell types using optical tweezers. Results show that stretching forces of tens of pN strongly promote Ca 2+ influx into filopodia, causing persistent Ca 2+ oscillations that last for minutes even after the force is released. Several known mechanosensitive Ca 2+ channels, such as Piezo 1, Piezo 2 and TRPV4, were found to be dispensable for the observed force-dependent Ca 2+ influx, while L-type Ca 2+ channels appear to be a key player in the discovered phenomenon. As previous studies have shown that intra-filopodial transient Ca 2+ signals play an important role in guidance of cell migration, our results suggest that the force-dependent activation of L-type Ca 2+ channels may contribute to this process. Overall, our study reveals an intricate interplay between mechanical forces and Ca 2+ signaling in filopodia, providing novel mechanistic insights for the force-dependent filopodia functions in guidance of cell migration. (Copyright © 2022 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Calcium signaling; Filopodia; L-type calcium channels; Mechanosensing; Optical tweezers |
| المشرفين على المادة: | SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20220408 Date Completed: 20220505 Latest Revision: 20220520 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.biomaterials.2022.121477 |
| PMID: | 35395455 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1878-5905 |
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| DOI: | 10.1016/j.biomaterials.2022.121477 |