دورية أكاديمية
أعرض في EDS

Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples.

التفاصيل البيبلوغرافية
العنوان: Evaluation of long-term stability of SARS-CoV-2 nucleic acid extracted from human nasopharyngeal samples.
المؤلفون: de Oliveira PSS; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: priusan@gmail.com., de Oliveira Silva B; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: barbaraoliveirabiomol@gmail.com., E Silva RP; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: romulo.nupit@gmail.com., Galdino LV; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: liliavieiragaldino@gmail.com., de Carvalho VMF; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: vanessamylenna@hotmail.com., de Almeida AR; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: anderson.ralmeida@ufpe.br., da Rosa MM; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: michelle.rosa@ufpe.br., de Melo Rêgo MJB; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: moacyr.rego@ufpe.br., da Rocha Pitta MG; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: mgrpitta@gmail.com., Pereira MC; Laboratory of Immunomodulation and New Therapeutic Approaches (LINAT), Suely-Galdino Therapeutic Innovation Research Center (NUPIT-SG), Federal University of Pernambuco (UFPE), Recife, PE, Brazil. Electronic address: michelly.pereira@ufpe.br.
المصدر: Journal of virological methods [J Virol Methods] 2022 Jul; Vol. 305, pp. 114529. Date of Electronic Publication: 2022 Apr 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
مواضيع طبية MeSH: COVID-19*/diagnosis , Nucleic Acids*, Humans ; RNA, Viral/analysis ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; Sensitivity and Specificity
مستخلص: The maintenance of SARS-Cov-2 RNA samples poses a new challenge for laboratories and researchers. In addition, it is a requirement in order to identify what strain of the new coronavirus is predominant in a region, for instance. Therefore, it is a must to keep the quality and viability of stored RNA to respond to this and other valid questions. In other to test the quality of our samples and storing protocols, we randomly checked RNA samples four different times over one year using a second RT-PCR assay after the first test. The virus genes, N1 and N2, showed no significant increase in the media of the CT value between the first assay and subsequent times with p > 0.05. However, the human RP gene showed differences in the first three times analyzed, but within the acceptable sample cut-off, according to the test manufacturer. After one year, the RNA extracted from human nasopharyngeal specimens are viable to detect the virus SARS-CoV-2 genes with minor changes.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
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فهرسة مساهمة: Keywords: Coronavirus; Quality-assurance; RT-qPCR
المشرفين على المادة: 0 (Nucleic Acids)
0 (RNA, Viral)
تواريخ الأحداث: Date Created: 20220410 Date Completed: 20220524 Latest Revision: 20221217
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8985402
DOI: 10.1016/j.jviromet.2022.114529
PMID: 35398116
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0984
DOI:10.1016/j.jviromet.2022.114529