دورية أكاديمية
أعرض في EDS

A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS-CoV-2 Spike S1 RBD produced in Escherichia coli.

التفاصيل البيبلوغرافية
العنوان: A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS-CoV-2 Spike S1 RBD produced in Escherichia coli.
المؤلفون: Conzentino MS; Setor Litoral, UFPR Matinhos, Rua Jaguariaíva, Tv. Caiobá, 512, Matinhos, 83260-000, PR, Brazil., Gonçalves ACA; Setor Litoral, UFPR Matinhos, Rua Jaguariaíva, Tv. Caiobá, 512, Matinhos, 83260-000, PR, Brazil., Paula NM; Setor Litoral, UFPR Matinhos, Rua Jaguariaíva, Tv. Caiobá, 512, Matinhos, 83260-000, PR, Brazil., Rego FGM; Post-Graduation Program in Pharmaceutical Sciences, UFPR, Curitiba, PR, Brazil., Zanette DL; Instituto Carlos Chagas - FioCruz, Curitiba, PR, Brazil., Aoki MN; Instituto Carlos Chagas - FioCruz, Curitiba, PR, Brazil., Nardin JM; Hospital Erasto Gaertner, Curitiba, PR, Brazil., Huergo LF; Setor Litoral, UFPR Matinhos, Rua Jaguariaíva, Tv. Caiobá, 512, Matinhos, 83260-000, PR, Brazil. luciano.huergo@gmail.com.
المصدر: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2022 Sep; Vol. 53 (3), pp. 1263-1269. Date of Electronic Publication: 2022 Apr 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2019- : Switzerland, AG : Springer International Publishing
Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia
مواضيع طبية MeSH: COVID-19* , SARS-CoV-2*/genetics, Antibodies, Viral ; Escherichia coli/genetics ; Humans ; Immunoassay/methods ; Immunoglobulin G ; Magnetic Phenomena ; Spike Glycoprotein, Coronavirus/genetics
مستخلص: Immunological assays to detect SARS-CoV-2 Spike Receptor Binding Domain (RBD) antigen seroconversion in humans are important tools to monitor the levels of protecting antibodies in the population in response to infection and/or immunization. Here we describe a simple, low cost, and high throughput Ni 2+ magnetic bead immunoassay to detect human IgG reactive to Spike S1 RBD Receptor Binding Domain produced in Escherichia coli. A 6xHis-tagged Spike S1 RBD was expressed in E. coli and purified by affinity chromatography. The protein was mobilized on the surface of Ni 2+ magnetic beads and used to investigate the presence of reactive IgG in the serum obtained from pre-pandemic and COVID-19 confirmed cases. The method was validated with a cohort of 290 samples and an area under the receiver operating characteristic curve of 0.94 was obtained. The method was operated with > 82% sensitivity at 98% specificity and was also able to track human IgG raised in response to vaccination with Comirnaty at > 85% sensitivity. The IgG signal obtained with the described method was well-correlated with the signal obtained when pre fusion Spike produced in HEK cell lines was used as antigen. This novel low-cost and high throughput immunoassay may act as an important tool to investigate protecting IgG antibodies against SARS-CoV-2 in the human population.
(© 2022. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
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فهرسة مساهمة: Keywords: COVID-19; High throughput; Magnetic beads; Magnetic immunoassay; SARS-CoV-2
المشرفين على المادة: 0 (Antibodies, Viral)
0 (Immunoglobulin G)
0 (Spike Glycoprotein, Coronavirus)
0 (spike protein, SARS-CoV-2)
تواريخ الأحداث: Date Created: 20220415 Date Completed: 20220908 Latest Revision: 20220908
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9009495
DOI: 10.1007/s42770-022-00753-x
PMID: 35426068
قاعدة البيانات: MEDLINE
الوصف
تدمد:1678-4405
DOI:10.1007/s42770-022-00753-x