دورية أكاديمية
أعرض في EDS

Influence of Needle Design and Irrigant Flow Rate on the Removal of Enterococcus faecalis Biofilms In Vitro.

التفاصيل البيبلوغرافية
العنوان: Influence of Needle Design and Irrigant Flow Rate on the Removal of Enterococcus faecalis Biofilms In Vitro.
المؤلفون: Provoost C; University Clinics of Dental Medicine, University of Geneva, 1207 Geneva, Switzerland., Rocca GT; University Clinics of Dental Medicine, University of Geneva, 1207 Geneva, Switzerland., Thibault A; University Clinics of Dental Medicine, University of Geneva, 1207 Geneva, Switzerland., Machtou P; University Clinics of Dental Medicine, University of Geneva, 1207 Geneva, Switzerland., Bouilllaguet S; University Clinics of Dental Medicine, University of Geneva, 1207 Geneva, Switzerland.
المصدر: Dentistry journal [Dent J (Basel)] 2022 Apr 02; Vol. 10 (4). Date of Electronic Publication: 2022 Apr 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101716125 Publication Model: Electronic Cited Medium: Internet ISSN: 2304-6767 (Electronic) Linking ISSN: 23046767 NLM ISO Abbreviation: Dent J (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: This study aimed to evaluate the influence of needle design and irrigant flow rate on the removal of Enterococcus faecalis mature biofilms during sodium hypochlorite irrigation. Forty-eight single-rooted human teeth were instrumented (ProTaper F3), autoclaved and inoculated with Enterococcus faecalis to establish a two-week-old biofilm. E. faecalis biofilms were treated with Sodium hypochlorite that was injected in the root canals using three types of needles (NaviTip, ProRinse, IrriFlex). For the IrriFlex needle, one, two, or four bars of pressure was applied to the irrigating solution to increase flow rates. Bacteria were labeled with the LIVE/DEAD BacLight Bacterial Viability kit, and viability was assessed by flow cytometry (FCM). Results were statistically analyzed using one-way ANOVA and Tukey multiple comparison intervals (α = 0.05). Bacterial viability was significantly reduced after sodium hypochlorite passive irrigation but the number of viable bacteria retrieved from root canal specimens irrigated with the Pro-Rinse needle was significantly higher compared to NaviTip and IrriFlex needles (p < 0.05). When the irrigant flow rate was increased, the viability of bacterial biofilms was significantly reduced compared to passive irrigation using the IrriFlex needle (p < 0.05). Applying higher flow rates during irrigation using the IrriFlex needle did not further reduce bacterial viability.
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فهرسة مساهمة: Keywords: E. faecalis biofilms; endodontic irrigation; needle
تواريخ الأحداث: Date Created: 20220421 Latest Revision: 20230308
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9030241
DOI: 10.3390/dj10040059
PMID: 35448053
قاعدة البيانات: MEDLINE
الوصف
تدمد:2304-6767
DOI:10.3390/dj10040059