دورية أكاديمية
أعرض في EDS

Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples.

التفاصيل البيبلوغرافية
العنوان: Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples.
المؤلفون: Breshears LE; Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA., Nguyen BT; Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA., Akarapipad P; Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA., Sosnowski K; Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA., Kaarj K; Department of Biosystems Engineering, The University of Arizona,Tucson, AZ 85721, USA., Quirk G; Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, AZ 85721, USA., Uhrlaub JL; Department of Immunobiology, The University of Arizona College of Medicine,Tucson, AZ 85724, USA., Nikolich-Žugich J; Department of Immunobiology, The University of Arizona College of Medicine,Tucson, AZ 85724, USA., Worobey M; Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson, AZ 85721, USA., Yoon JY
المصدر: PNAS nexus [PNAS Nexus] 2022 Mar 16; Vol. 1 (1), pp. pgac028. Date of Electronic Publication: 2022 Mar 16 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press on behalf of the National Academy of Sciences Country of Publication: England NLM ID: 9918367777906676 Publication Model: eCollection Cited Medium: Internet ISSN: 2752-6542 (Electronic) Linking ISSN: 27526542 NLM ISO Abbreviation: PNAS Nexus Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Oxford] : Oxford University Press on behalf of the National Academy of Sciences, [2022]-
مستخلص: Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n  = 27 human subjects with n  = 13 RT-qPCR positives.
(© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.)
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معلومات مُعتمدة: T32 GM132008 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: COVID-19; biosensor; clinical samples; immunoassay; microfluidics; point-of-care; smartphone; smartphone-based fluorescence microscope
تواريخ الأحداث: Date Created: 20220422 Latest Revision: 20240211
رمز التحديث: 20240211
مُعرف محوري في PubMed: PMC9013775
DOI: 10.1093/pnasnexus/pgac028
PMID: 35450423
قاعدة البيانات: MEDLINE
الوصف
تدمد:2752-6542
DOI:10.1093/pnasnexus/pgac028