دورية أكاديمية
أعرض في EDS

Expression of Staphylococcal Virulence Genes In Situ in Human Skin and Soft Tissue Infections.

التفاصيل البيبلوغرافية
العنوان: Expression of Staphylococcal Virulence Genes In Situ in Human Skin and Soft Tissue Infections.
المؤلفون: Pulia MS; Department of Emergency Medicine, University of Wisconsin, School of Medicine and Public Health, Madison, WI 53726, USA., Anderson J; Integrated Research Development Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Ye Z; Bioinformatics Research Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Elsayed NS; Center for Precision Medicine Research, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Le T; Integrated Research Development Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Patitucci J; Bioinformatics Research Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Ganta K; Center for Precision Medicine Research, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Hall M; Department of Infectious Diseases, Marshfield Clinic Health System, Marshfield, WI 54449, USA., Singh VK; Department of Microbiology and Immunology, Kirksville College of Osteopathic Medicine, A.T. Still, University of Health Sciences, Kirksville, MO 63501, USA., Shukla SK; Center for Precision Medicine Research, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA.
المصدر: Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2022 Apr 14; Vol. 11 (4). Date of Electronic Publication: 2022 Apr 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101637404 Publication Model: Electronic Cited Medium: Print ISSN: 2079-6382 (Print) Linking ISSN: 20796382 NLM ISO Abbreviation: Antibiotics (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2012-
مستخلص: Background: Staphylococcus aureus , the most common pathogen in skin and soft tissue infections (SSTI), harbors many well-characterized virulence genes. However, the expression of many of them in SSTIs is unknown. In this study, S. aureus virulence genes expressed in SSTI were investigated.
Methods: Fifty-three subjects presenting to the outpatient's care and emergency departments with a purulent SSTI at two medical centers in Wisconsin, USA, were enrolled in the study. Total mRNA was extracted from the purulent or swab materials, made into cDNA and sequenced on MiSeq platform. The relative cDNA counts to gmk and identifications of the transcripts were carried out with respect to USA300 reference genome and using SAMTOOLS v.1.3 and BWA, respectively.
Result: A significantly higher cDNA count was observed for many of the virulence and regulatory gene transcripts in the pus samples compared to the swab samples relative to the cDNA counts for gmk , a housekeeping gene. They were for luk S- PV (18.6 vs. 14.2), isa A (13.4 vs. 8.5), ssa A (4.8 vs. 3.1), hlg C (1.4 vs. 1.33), atl (17.7 vs. 8.33), clfA (3.9 vs. 0.83), eno (6.04 vs. 3.16), fnbA (5.93 vs. 0.33), saeS (6.3 vs. 1.33), saeR (5.4 vs. 3.33) and agrC (5.6 vs. 1.5).
Conclusions: A relative increase in the transcripts of several toxins, adhesion and regulatory genes with respect to a gmk in purulent materials suggests their role in situ during SSTIs, perhaps in an orchestrated manner.
References: FEMS Immunol Med Microbiol. 2003 Aug 18;38(1):23-8. (PMID: 12900051)
Microbiology (Reading). 2008 Feb;154(Pt 2):550-558. (PMID: 18227259)
Curr Opin Microbiol. 2013 Feb;16(1):63-9. (PMID: 23466211)
Infect Immun. 2002 Jan;70(1):127-33. (PMID: 11748173)
Nat Rev Microbiol. 2017 Jul;15(7):435-447. (PMID: 28420883)
Infect Immun. 2011 Mar;79(3):1153-65. (PMID: 21189325)
Curr Top Microbiol Immunol. 2017;409:325-383. (PMID: 28900682)
J Bacteriol. 2003 Nov;185(21):6278-86. (PMID: 14563862)
BMC Infect Dis. 2018 Jan 25;18(1):51. (PMID: 29370771)
Mol Microbiol. 1998 Oct;30(2):245-57. (PMID: 9791170)
J Clin Microbiol. 2009 May;47(5):1344-51. (PMID: 19321725)
Clin Infect Dis. 1999 Nov;29(5):1128-32. (PMID: 10524952)
Microbiology (Reading). 2003 Oct;149(Pt 10):2709-2717. (PMID: 14523104)
J Clin Microbiol. 2005 Jul;43(7):3373-9. (PMID: 16000462)
Proc Natl Acad Sci U S A. 2018 May 22;115(21):5564-5569. (PMID: 29735708)
PLoS One. 2013;8(1):e52722. (PMID: 23300988)
J Infect Dis. 2014 May 15;209(10):1542-50. (PMID: 24286981)
Proc Natl Acad Sci U S A. 2014 May 13;111(19):E2037-45. (PMID: 24782537)
Proc Natl Acad Sci U S A. 2021 Nov 2;118(44):. (PMID: 34716264)
PeerJ. 2019 Oct 11;7:e7755. (PMID: 31616586)
PLoS One. 2012;7(6):e40353. (PMID: 22768285)
Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5587-92. (PMID: 20231457)
J Clin Microbiol. 2010 Oct;48(10):3582-92. (PMID: 20668125)
Toxicon. 2001 Nov;39(11):1661-72. (PMID: 11595629)
Infect Immun. 2004 Oct;72(10):5668-75. (PMID: 15385465)
Curr Opin Infect Dis. 2016 Apr;29(2):109-15. (PMID: 26779772)
J Biol Chem. 2004 Dec 3;279(49):50691-9. (PMID: 15385531)
Rev Med Suisse. 2007 May 2;3(109):1106-8, 1110-1. (PMID: 17552268)
Infect Dis Clin North Am. 2015 Sep;29(3):429-64. (PMID: 26311356)
EMBO J. 1995 Sep 15;14(18):4569-77. (PMID: 7556100)
J Med Microbiol. 2017 Dec;66(12):1811-1821. (PMID: 29099691)
J Bacteriol. 2007 Oct;189(20):7316-25. (PMID: 17675373)
Lancet. 2002 May 25;359(9320):1819-27. (PMID: 12044378)
J Clin Microbiol. 2004 Aug;42(8):3752-7. (PMID: 15297526)
Anal Chem. 2018 Sep 4;90(17):10400-10408. (PMID: 30091898)
PLoS One. 2010 Dec 02;5(12):e15177. (PMID: 21151999)
Infect Immun. 2002 Feb;70(2):631-41. (PMID: 11796592)
N Engl J Med. 2006 Aug 17;355(7):666-74. (PMID: 16914702)
J Invest Dermatol. 2011 Apr;131(4):907-15. (PMID: 21191403)
Infect Immun. 2011 Jun;79(6):2215-23. (PMID: 21422173)
BMC Infect Dis. 2010 May 26;10:133. (PMID: 20500885)
FEMS Microbiol Lett. 2010 Jul;308(2):175-84. (PMID: 20528938)
Biosci Biotechnol Biochem. 2004 May;68(5):981-1003. (PMID: 15170101)
Antimicrob Agents Chemother. 2004 Aug;48(8):3080-5. (PMID: 15273123)
mBio. 2017 Dec 5;8(6):. (PMID: 29208742)
Emerg Infect Dis. 2006 Jul;12(7):1174-5. (PMID: 16848048)
Mol Microbiol. 2006 Aug;61(4):1038-48. (PMID: 16879652)
PLoS One. 2013 Dec 12;8(12):e83368. (PMID: 24349495)
Toxins (Basel). 2014 Mar 04;6(3):950-72. (PMID: 24599233)
J Clin Microbiol. 2004 Feb;42(2):792-9. (PMID: 14766855)
Int J Infect Dis. 2013 Feb;17(2):e115-9. (PMID: 23092752)
Int J Med Microbiol. 2015 Jan;305(1):55-64. (PMID: 25466204)
Clin Infect Dis. 2008 Jun 1;46 Suppl 5:S350-9. (PMID: 18462090)
J Clin Microbiol. 2015 Mar;53(3):810-5. (PMID: 25540401)
Clin Diagn Lab Immunol. 2001 Mar;8(2):279-82. (PMID: 11238208)
Clin Infect Dis. 2005 Feb 15;40(4):562-73. (PMID: 15712079)
J Clin Microbiol. 2006 Apr;44(4):1555-7. (PMID: 16597892)
BMC Infect Dis. 2017 Jun 7;17(1):405. (PMID: 28592231)
JAMA. 2003 Dec 10;290(22):2976-84. (PMID: 14665659)
Infect Immun. 2005 Aug;73(8):4793-802. (PMID: 16040992)
Lancet Infect Dis. 2005 Dec;5(12):751-62. (PMID: 16310147)
Emerg Infect Dis. 2004 Jan;10(1):121-4. (PMID: 15078606)
FEMS Immunol Med Microbiol. 2000 Oct;29(2):145-53. (PMID: 11024354)
J Microbiol Methods. 2004 Dec;59(3):363-70. (PMID: 15488279)
J Infect Dis. 2009 Feb 1;199(3):294-301. (PMID: 19115951)
J Biol Chem. 2004 Sep 24;279(39):41028-37. (PMID: 15262988)
Proc Natl Acad Sci U S A. 2011 Oct 18;108(42):17314-9. (PMID: 21969538)
Braz J Infect Dis. 2015 Nov-Dec;19(6):614-22. (PMID: 26408338)
Int J Biol Macromol. 2018 Apr 15;110:179-184. (PMID: 29398086)
Clin Med Res. 2015 Mar;13(1):7-11. (PMID: 24899694)
Clin Microbiol Rev. 1997 Jul;10(3):505-20. (PMID: 9227864)
Infect Immun. 2017 May 23;85(6):. (PMID: 28373353)
Microbiol Mol Biol Rev. 2014 Jun;78(2):199-230. (PMID: 24847020)
Curr Opin Microbiol. 2006 Feb;9(1):5-11. (PMID: 16406780)
فهرسة مساهمة: Keywords: Panton–Valentine leukocidin; SSTI; Staphylococcus aureus; accessory gene regulators; clumping factors; hemolysins
تواريخ الأحداث: Date Created: 20220423 Latest Revision: 20220716
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9032627
DOI: 10.3390/antibiotics11040527
PMID: 35453277
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6382
DOI:10.3390/antibiotics11040527