دورية أكاديمية
Expression of Staphylococcal Virulence Genes In Situ in Human Skin and Soft Tissue Infections.
العنوان: | Expression of Staphylococcal Virulence Genes In Situ in Human Skin and Soft Tissue Infections. |
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المؤلفون: | Pulia MS; Department of Emergency Medicine, University of Wisconsin, School of Medicine and Public Health, Madison, WI 53726, USA., Anderson J; Integrated Research Development Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Ye Z; Bioinformatics Research Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Elsayed NS; Center for Precision Medicine Research, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Le T; Integrated Research Development Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Patitucci J; Bioinformatics Research Center, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Ganta K; Center for Precision Medicine Research, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA., Hall M; Department of Infectious Diseases, Marshfield Clinic Health System, Marshfield, WI 54449, USA., Singh VK; Department of Microbiology and Immunology, Kirksville College of Osteopathic Medicine, A.T. Still, University of Health Sciences, Kirksville, MO 63501, USA., Shukla SK; Center for Precision Medicine Research, Marshfield Clinic Research Institute, Marshfield, WI 54449, USA. |
المصدر: | Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2022 Apr 14; Vol. 11 (4). Date of Electronic Publication: 2022 Apr 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101637404 Publication Model: Electronic Cited Medium: Print ISSN: 2079-6382 (Print) Linking ISSN: 20796382 NLM ISO Abbreviation: Antibiotics (Basel) Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, 2012- |
مستخلص: | Background: Staphylococcus aureus , the most common pathogen in skin and soft tissue infections (SSTI), harbors many well-characterized virulence genes. However, the expression of many of them in SSTIs is unknown. In this study, S. aureus virulence genes expressed in SSTI were investigated. Methods: Fifty-three subjects presenting to the outpatient's care and emergency departments with a purulent SSTI at two medical centers in Wisconsin, USA, were enrolled in the study. Total mRNA was extracted from the purulent or swab materials, made into cDNA and sequenced on MiSeq platform. The relative cDNA counts to gmk and identifications of the transcripts were carried out with respect to USA300 reference genome and using SAMTOOLS v.1.3 and BWA, respectively. Result: A significantly higher cDNA count was observed for many of the virulence and regulatory gene transcripts in the pus samples compared to the swab samples relative to the cDNA counts for gmk , a housekeeping gene. They were for luk S- PV (18.6 vs. 14.2), isa A (13.4 vs. 8.5), ssa A (4.8 vs. 3.1), hlg C (1.4 vs. 1.33), atl (17.7 vs. 8.33), clfA (3.9 vs. 0.83), eno (6.04 vs. 3.16), fnbA (5.93 vs. 0.33), saeS (6.3 vs. 1.33), saeR (5.4 vs. 3.33) and agrC (5.6 vs. 1.5). Conclusions: A relative increase in the transcripts of several toxins, adhesion and regulatory genes with respect to a gmk in purulent materials suggests their role in situ during SSTIs, perhaps in an orchestrated manner. |
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فهرسة مساهمة: | Keywords: Panton–Valentine leukocidin; SSTI; Staphylococcus aureus; accessory gene regulators; clumping factors; hemolysins |
تواريخ الأحداث: | Date Created: 20220423 Latest Revision: 20220716 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9032627 |
DOI: | 10.3390/antibiotics11040527 |
PMID: | 35453277 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2079-6382 |
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DOI: | 10.3390/antibiotics11040527 |