دورية أكاديمية
أعرض في EDS

Group B Streptococcus -Induced Macropinocytosis Contributes to Bacterial Invasion of Brain Endothelial Cells.

التفاصيل البيبلوغرافية
العنوان: Group B Streptococcus -Induced Macropinocytosis Contributes to Bacterial Invasion of Brain Endothelial Cells.
المؤلفون: Espinal ER; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA., Matthews T; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA., Holder BM; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA., Bee OB; Department of Chemical and Biological Engineering, University of Wisconsin, Madison, WI 53706, USA., Humber GM; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA., Brook CE; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA., Divyapicigil M; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA., Sharp J; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA., Kim BJ; Department of Biological Sciences, University of Alabama, 300 Hackberry Lane, Tuscaloosa, AL 35405, USA.; Department of Microbiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.; Center for Convergent Biosciences and Medicine, University of Alabama, Tuscaloosa, AL 35487, USA.; Alabama Life Research Institute, University of Alabama, Tuscaloosa, AL 35487, USA.
المصدر: Pathogens (Basel, Switzerland) [Pathogens] 2022 Apr 15; Vol. 11 (4). Date of Electronic Publication: 2022 Apr 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596317 Publication Model: Electronic Cited Medium: Print ISSN: 2076-0817 (Print) Linking ISSN: 20760817 NLM ISO Abbreviation: Pathogens Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2012-
مستخلص: Bacterial meningitis is defined as serious inflammation of the central nervous system (CNS) in which bacteria infect the blood-brain barrier (BBB), a network of highly specialized brain endothelial cells (BECs). Dysfunction of the BBB is a hallmark of bacterial meningitis. Group B Streptococcus (GBS) is one of the leading organisms that cause bacterial meningitis, especially in neonates. Macropinocytosis is an actin-dependent form of endocytosis that is also tightly regulated at the BBB. Previous studies have shown that inhibition of actin-dependent processes decreases bacterial invasion, suggesting that pathogens can utilize macropinocytotic pathways for invasion. The purpose of this project is to study the factors that lead to dysfunction of the BBB. We demonstrate that infection with GBS increases rates of endocytosis in BECs. We identified a potential pathway, PLC-PKC-Nox2, in BECs that contributes to macropinocytosis regulation. Here we demonstrate that downstream inhibition of PLC, PKC, or Nox2 significantly blocks GBS invasion of BECs. Additionally, we show that pharmacological activation of PKC can turn on macropinocytosis and increase bacterial invasion of nonpathogenic yet genetically similar Lactococcus lactis . Our results suggest that GBS activates BEC signaling pathways that increase rates of macropinocytosis and subsequently the invasion of GBS.
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فهرسة مساهمة: Keywords: bacterial meningitis; blood–brain barrier; group B Streptococcus; host–pathogen interaction; iPSC-BEC; macropinocytosis
تواريخ الأحداث: Date Created: 20220423 Latest Revision: 20220429
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9028350
DOI: 10.3390/pathogens11040474
PMID: 35456149
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-0817
DOI:10.3390/pathogens11040474