دورية أكاديمية
RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters.
العنوان: | RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters. |
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المؤلفون: | Glotov AS; Laboratory of Biobanking and Genome Medicine, Institute of Translational Biomedicine, Saint Petersburg State University, Universitetskaya nab., 7-9-11, 199034 St. Petersburg, Russia.; Division of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, Russia., Zelenkova IE; GENUD Research Group, FIMS Collaborating Center of Sports Medicine, Department of Physiatry and Nursing, University of Zaragoza, 50009 Zaragoza, Spain.; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia, 119435 Moscow, Russia., Vashukova ES; Division of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, Russia., Shuvalova AR; Division of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, Russia., Zolotareva AD; Division of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, Russia., Polev DE; Division of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, Russia., Barbitoff YA; Division of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, Russia.; Bioinformatics Institute, Kantemirovskaya St., 2A, 194100 St. Petersburg, Russia., Glotov OS; Division of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, Russia.; Children's Scientific and Clinical Center for Infectious Diseases of the Federal Medical and Biological Agency, 197022 St. Petersburg, Russia., Sarana AM; City Hospital #40, Sestroretsk, Borisov Str., 9, 197706 St. Petersburg, Russia.; Department of Postgraduate Medical Education, St. Petersburg State University, Universitetskaya nab., 7-9-11, 199034 St. Petersburg, Russia., Shcherbak SG; City Hospital #40, Sestroretsk, Borisov Str., 9, 197706 St. Petersburg, Russia.; Department of Postgraduate Medical Education, St. Petersburg State University, Universitetskaya nab., 7-9-11, 199034 St. Petersburg, Russia., Rozina MA; Russian Skating Union, Luzhnetskaya nab., 8, Office 230, 119991 Moscow, Russia., Gogotova VL; Russian Skating Union, Luzhnetskaya nab., 8, Office 230, 119991 Moscow, Russia., Predeus AV; Bioinformatics Institute, Kantemirovskaya St., 2A, 194100 St. Petersburg, Russia. |
المصدر: | Genes [Genes (Basel)] 2022 Mar 24; Vol. 13 (4). Date of Electronic Publication: 2022 Mar 24. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel : MDPI |
مواضيع طبية MeSH: | Altitude* , Exercise*/physiology, Acclimatization ; Athletes ; Female ; Humans ; Sequence Analysis, RNA |
مستخلص: | Although high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profiling of whole blood. Seven elite female speed skaters were profiled on the 18th day of high-altitude adaptation. Whole blood RNA-seq before and after an intense 1 h skating bout was used to measure gene expression changes associated with exercise. In order to identify the genes specifically regulated at high altitudes, we have leveraged the data from eight previously published microarray datasets studying blood expression changes after exercise at sea level. Using cell type-specific signatures, we were able to deconvolute changes of cell type abundance from individual gene expression changes. Among these were PHOSPHO1 , with a known role in erythropoiesis, and MARC1 with a role in endogenic NO metabolism. We find that platelet and erythrocyte counts uniquely respond to altitude exercise, while changes in neutrophils represent a more generic marker of intense exercise. Publicly available data from both single cell atlases and exercise-related blood profiling dramatically increases the value of whole blood RNA-seq for the dynamic evaluation of physiological changes in an athlete's body. |
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فهرسة مساهمة: | Keywords: RNA sequencing; RNA-seq; elite athletes; exercise; expression profiling; high altitude adaptation; live high; platelets; speed skating; train high (LHTH); whole blood |
تواريخ الأحداث: | Date Created: 20220423 Date Completed: 20220426 Latest Revision: 20220716 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9027771 |
DOI: | 10.3390/genes13040574 |
PMID: | 35456380 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2073-4425 |
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DOI: | 10.3390/genes13040574 |