دورية أكاديمية
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Add Sugar to Chitosan: Mucoadhesion and In Vitro Intestinal Permeability of Mannosylated Chitosan Nanocarriers.

التفاصيل البيبلوغرافية
العنوان: Add Sugar to Chitosan: Mucoadhesion and In Vitro Intestinal Permeability of Mannosylated Chitosan Nanocarriers.
المؤلفون: Ejaz S; Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad 45550, Pakistan.; School of Veterinary Medicine, University College Dublin (UCD), Belfield, D04 W6F6 Dublin, Ireland., Hogg B; School of Veterinary Medicine, University College Dublin (UCD), Belfield, D04 W6F6 Dublin, Ireland., Hristov DR; School of Veterinary Medicine, University College Dublin (UCD), Belfield, D04 W6F6 Dublin, Ireland., Brayden DJ; School of Veterinary Medicine, University College Dublin (UCD), Belfield, D04 W6F6 Dublin, Ireland.; Conway Institute of Biomolecular and Biomedical Research, University College Dublin (UCD), Belfield, D04 V1W8 Dublin, Ireland., Imran M; Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad 45550, Pakistan., Bhattacharjee S; School of Veterinary Medicine, University College Dublin (UCD), Belfield, D04 W6F6 Dublin, Ireland.
المصدر: Pharmaceutics [Pharmaceutics] 2022 Apr 11; Vol. 14 (4). Date of Electronic Publication: 2022 Apr 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101534003 Publication Model: Electronic Cited Medium: Print ISSN: 1999-4923 (Print) Linking ISSN: 19994923 NLM ISO Abbreviation: Pharmaceutics Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مستخلص: Crosslinked chitosan nanocarriers (140-160 nm) entrapping coumarin-6 ( λ ex/em = 455/508 nm) with or without surface mannosylation were synthesized and assessed for cytotoxicity, adherence and cellular uptake in Caco-2 cells, flux across Caco-2 monolayers, and mucoadhesion to porcine mucin. Mannosylated and non-mannosylated nanocarriers demonstrated biocompatibility with slow release of coumarin-6 at pH 6.8 and 7.4 over 24 h. Adherence of the non-mannosylated nanocarriers (50 and 150 µg/mL) to Caco-2 cells was ~10% over 24 h, whereas cellular uptake of 25-30% was noted at 4 h. The mannosylated nanocarriers showed a similar adherence to non-mannosylated nanocarriers after 24 h, but a lower cellular uptake (~20%) at 1 h, comparable uptake at 4 h, and a higher uptake (~25-30%) at 24 h. Overall, the nanocarriers did not affect the integrity of Caco-2 monolayers. Mannosylated nanocarriers elicited higher P app of 1.6 × 10 -6 cm/s (50 µg/mL) and 1.2 × 10 -6 (150 µg/mL) than the non-mannosylated ones: 9.8 × 10 -7 cm/s (50 µg/mL) and 1.0 × 10 -6 (150 µg/mL) after 2 h. Non-mannosylated chitosan nanocarriers elicited enhanced adhesion to porcine gut mucin via mucin-filled microchannels due to higher cationic charge density. These results underpin the importance of surface chemistry in the biological interactions of nanocarriers, while highlighting the role of surface hydrophilicity in mucopermeation due to mannosylation.
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معلومات مُعتمدة: scholarship (Award No. I-8/HEC/HRD/2019/8762) Higher Education Commission Pakistan via the International Research Support Initiative Program (IRSIP); 13/RC/2073 P2 Science Foundation Ireland, the CÚRAM Centre for Medical Devices and the EU Regional Development Fund.
فهرسة مساهمة: Keywords: bioconjugation; chitosan nanoparticles; mannosylation; mucoadhesion; surface charge density; surface functionalization
تواريخ الأحداث: Date Created: 20220423 Latest Revision: 20220716
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9024478
DOI: 10.3390/pharmaceutics14040830
PMID: 35456664
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4923
DOI:10.3390/pharmaceutics14040830