دورية أكاديمية
Dynamics of a Staphylococcus aureus infective endocarditis simulation model.
| العنوان: | Dynamics of a Staphylococcus aureus infective endocarditis simulation model. |
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| المؤلفون: | Schwartz FA; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark., Nielsen L; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.; Department of Technology, Faculty of Health, University College Copenhagen, Copenhagen, Denmark., Struve Andersen J; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.; Department of Technology, Faculty of Health, University College Copenhagen, Copenhagen, Denmark., Bock M; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark., Christophersen L; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark., Sunnerhagen T; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.; Section for Infection Medicine, Department of Sciences Lund, Lund University, Lund, Sweden.; Department of Clinical Microbiology, Office for Medical Services, Lund, Sweden., Lerche CJ; Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark., Bay L; Department of Immunology and Microbiology, Costerton Biofilm Center, University of Copenhagen, Copenhagen, Denmark., Bundgaard H; Department of Cardiology, Copenhagen University Hospital Herlev, Copenhagen, Denmark.; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Høiby N; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.; Department of Immunology and Microbiology, Costerton Biofilm Center, University of Copenhagen, Copenhagen, Denmark., Moser C; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.; Department of Immunology and Microbiology, Costerton Biofilm Center, University of Copenhagen, Copenhagen, Denmark. |
| المصدر: | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2022 Aug; Vol. 130 (8), pp. 515-523. Date of Electronic Publication: 2022 May 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Munksgaard Country of Publication: Denmark NLM ID: 8803400 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0463 (Electronic) Linking ISSN: 09034641 NLM ISO Abbreviation: APMIS Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Copenhagen : Munksgaard Original Publication: Copenhagen : Munksgaard, c1988- |
| مواضيع طبية MeSH: | Endocarditis*/drug therapy , Endocarditis, Bacterial*/drug therapy , Endocarditis, Bacterial*/microbiology , Staphylococcal Infections*/drug therapy , Staphylococcal Infections*/microbiology, Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacteria ; Biofilms ; Humans ; Staphylococcus aureus |
| مستخلص: | Infective endocarditis (IE) is a serious infection of the inner surface of heart, resulting from minor lesions in the endocardium. The damage induces a healing reaction, which leads to recruitment of fibrin and immune cells. This sterile healing vegetation can be colonized during temporary bacteremia, inducing IE. We have previously established a novel in vitro IE model using a simulated IE vegetation (IEV) model produced from whole venous blood, on which we achieved stable bacterial colonization after 24 h. The bacteria were organized in biofilm aggregates and displayed increased tolerance toward antibiotics. In this current study, we aimed at further characterizing the time course of biofilm formation and the impact on antibiotic tolerance development. We found that a Staphylococcus aureus reference strain, as well as three clinical IE isolates formed biofilms on the IEV after 6 h. When treatment was initiated immediately after infection, the antibiotic effect was significantly higher than when treatment was started after the biofilm was allowed to mature. We could follow the biofilm development microscopically by visualizing growing bacterial aggregates on the IEV. The findings indicate that mature, antibiotic-tolerant biofilms can be formed in our model already after 6 h, accelerating the screening for optimal treatment strategies for IE. (© 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.) |
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| معلومات مُعتمدة: | E-22416-07 Rigshospitalet; TZ2021-0008 Thelma Zoégas Fund for Medical Research; 42001 Royal Physiographic Society of Lund; BA21-0430 Längmanska Foundation; RMv2021-0002 Lund University; RMh2021-0001 Lund University; LA2021-0027 Sigurd and Elsa Golje; Tornspiran Foundation; PD20-0031 Swedish Society for Medical Research; 19-L-0283 A.P. Møller Fonden; NNF17OC0025074 Novo Nordisk Fonden |
| فهرسة مساهمة: | Keywords: Biofilm; antibiotic tolerance; histopathology; host response; infective endocarditis; organoid |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20220423 Date Completed: 20220712 Latest Revision: 20221015 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9545761 |
| DOI: | 10.1111/apm.13231 |
| PMID: | 35460117 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1600-0463 |
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| DOI: | 10.1111/apm.13231 |