دورية أكاديمية
أعرض في EDS

SARS-CoV-2 infection of human-induced pluripotent stem cells-derived lung lineage cells evokes inflammatory and chemosensory responses by targeting mitochondrial pathways.

التفاصيل البيبلوغرافية
العنوان: SARS-CoV-2 infection of human-induced pluripotent stem cells-derived lung lineage cells evokes inflammatory and chemosensory responses by targeting mitochondrial pathways.
المؤلفون: Surendran H; Eyestem Research, Centre for Cellular and Molecular Platforms (C-CAMP), Bengaluru, Karnataka, India., Kumar S; Clinical and Cellular Virology Laboratory, Translational Health Science and Technology Institute (THSTI), Faridabad, Haryana, India., Narasimhaiah S; Eyestem Research, Centre for Cellular and Molecular Platforms (C-CAMP), Bengaluru, Karnataka, India., Ananthamurthy A; St John's Medical College, Bengaluru, Karnataka, India., Varghese PS; St John's Medical College, Bengaluru, Karnataka, India., D'Souza GA; St John's Medical College, Bengaluru, Karnataka, India., Medigeshi G; Clinical and Cellular Virology Laboratory, Translational Health Science and Technology Institute (THSTI), Faridabad, Haryana, India., Pal R; Eyestem Research, Centre for Cellular and Molecular Platforms (C-CAMP), Bengaluru, Karnataka, India.; The University of Trans-disciplinary Health Sciences and Technology (TDU), Bengaluru, Karnataka, India.
المصدر: Journal of cellular physiology [J Cell Physiol] 2022 Jul; Vol. 237 (7), pp. 2913-2928. Date of Electronic Publication: 2022 Apr 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4652 (Electronic) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Wiley-Liss
Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology.
مواضيع طبية MeSH: COVID-19*/immunology , COVID-19*/pathology , Induced Pluripotent Stem Cells*/pathology , Induced Pluripotent Stem Cells*/virology, Adult ; Cytokines ; Humans ; Lung/pathology ; Lung/virology ; Mitochondria/metabolism ; Reactive Oxygen Species ; SARS-CoV-2
مستخلص: The COVID-19 disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily affects the lung, particularly the proximal airway and distal alveolar cells. NKX2.1+ primordial lung progenitors of the foregut (anterior) endoderm are the developmental precursors to all adult lung epithelial lineages and are postulated to play an important role in viral tropism. Here, we show that SARS-CoV-2 readily infected and replicated in human-induced pluripotent stem cell-derived proximal airway cells, distal alveolar cells, and lung progenitors. In addition to the upregulation of antiviral defense and immune responses, transcriptomics data uncovered a robust epithelial cell-specific response, including perturbation of metabolic processes and disruption in the alveolar maturation program. We also identified spatiotemporal dysregulation of mitochondrial heme oxygenase 1 (HMOX1), which is associated with defense against antioxidant-induced lung injury. Cytokines, such as TNF-α, INF-γ, IL-6, and IL-13, were upregulated in infected cells sparking mitochondrial ROS production and change in electron transport chain complexes. Increased mitochondrial ROS then activated additional proinflammatory cytokines leading to an aberrant cell cycle resulting in apoptosis. Notably, we are the first to report a chemosensory response resulting from SARS-CoV-2 infection similar to that seen in COVID-19 patients. Some of our key findings were validated using COVID-19-affected postmortem lung tissue sections. These results suggest that our in vitro system could serve as a suitable model to investigate the pathogenetic mechanisms of SARS-CoV-2 infection and to discover and test therapeutic drugs against COVID-19 or its consequences.
(© 2022 Wiley Periodicals LLC.)
References: Cancer Biol Ther. 2010 Mar 15;9(6):417-22. (PMID: 20190564)
Cell Stem Cell. 2020 Dec 3;27(6):890-904.e8. (PMID: 33128895)
Curr Protoc Stem Cell Biol. 2019 Jun;49(1):e86. (PMID: 30997959)
Semin Cell Dev Biol. 2013 Mar;24(3):240-6. (PMID: 22963927)
Stem Cells Dev. 2020 Nov 1;29(21):1365-1369. (PMID: 32867617)
Cell Rep. 2021 May 4;35(5):109055. (PMID: 33905739)
Protein Cell. 2021 Sep;12(9):717-733. (PMID: 33314005)
J Cell Commun Signal. 2009 Dec;3(3-4):287-310. (PMID: 19838819)
Neuron. 2020 Jul 22;107(2):219-233. (PMID: 32640192)
Science. 2016 Feb 12;351(6274):707-10. (PMID: 26743624)
Front Aging Neurosci. 2021 Jan 12;12:614650. (PMID: 33510633)
Stem Cells. 2018 Feb;36(2):218-229. (PMID: 29143419)
Am J Respir Cell Mol Biol. 2014 Apr;50(4):678-83. (PMID: 24219573)
Lab Invest. 2006 May;86(5):425-44. (PMID: 16568108)
Cell Syst. 2020 Jul 22;11(1):102-108.e3. (PMID: 32673562)
Pflugers Arch. 2019 Apr;471(4):519-532. (PMID: 30397774)
Stem Cells. 2021 Oct;39(10):1310-1321. (PMID: 34152044)
Thorax. 2010 Aug;65(8):733-8. (PMID: 20685750)
mBio. 2014 May 20;5(3):e01174-14. (PMID: 24846384)
Mucosal Immunol. 2013 Jul;6(4):762-75. (PMID: 23187315)
iScience. 2020 May 22;23(5):101083. (PMID: 32380423)
Chem Senses. 2009 Nov;34(9):789-97. (PMID: 19833661)
Science. 2009 Aug 28;325(5944):1131-4. (PMID: 19628819)
Cell Stem Cell. 2020 Dec 3;27(6):962-973.e7. (PMID: 32979316)
Dtsch Arztebl Int. 2020 Jul 20;117(29-30):500-506. (PMID: 32865490)
Sci Rep. 2018 Aug 28;8(1):12983. (PMID: 30154568)
Nat Med. 2014 Aug;20(8):822-32. (PMID: 25100528)
J Neurosci. 2003 Sep 10;23(23):8291-301. (PMID: 12967991)
J Cell Physiol. 2022 Jul;237(7):2913-2928. (PMID: 35460571)
J Clin Invest. 2012 Nov;122(11):4145-59. (PMID: 23041624)
Sci Rep. 2020 Oct 20;10(1):17772. (PMID: 33082395)
Am J Respir Cell Mol Biol. 2007 Feb;36(2):158-65. (PMID: 16980551)
J Clin Virol. 2022 Jan;146:105060. (PMID: 34971849)
Front Endocrinol (Lausanne). 2021 Nov 18;12:747744. (PMID: 34867791)
Am J Respir Cell Mol Biol. 2014 Mar;50(3):637-46. (PMID: 24134460)
Indian J Tuberc. 2021 Jul;68(3):330-333. (PMID: 34099197)
Science. 2005 Dec 2;310(5753):1495-9. (PMID: 16322458)
J Neurophysiol. 2008 Jun;99(6):2929-37. (PMID: 18417634)
Am J Respir Crit Care Med. 2005 Sep 15;172(6):660-70. (PMID: 15901614)
Cell Rep. 2021 Apr 20;35(3):109011. (PMID: 33882306)
Immunol Rev. 2021 Jul;302(1):228-240. (PMID: 34028807)
Dev Biol. 2009 Apr 15;328(2):529-40. (PMID: 19389376)
Biochim Biophys Acta. 2013 Jan;1833(1):225-32. (PMID: 22440325)
Mitochondrion. 2020 Sep;54:1-7. (PMID: 32574708)
Am J Physiol Cell Physiol. 2020 Aug 1;319(2):C258-C267. (PMID: 32510973)
Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):E1723-30. (PMID: 24706852)
Nature. 2020 Jul;583(7816):459-468. (PMID: 32353859)
Nature. 2018 Aug;560(7718):319-324. (PMID: 30069044)
معلومات مُعتمدة: BT/COVID0046/01/20 COVID-19 Research Consortium, Department of Biotechnology (DBT) and Biotechnology Industry Research Assistance Council (BIRAC), Government of India
فهرسة مساهمة: Keywords: SARS-CoV-2; chemosensory response; induced pluripotent stem cells; inflammatory response; lung epithelial cells; mitochondrial damage
المشرفين على المادة: 0 (Cytokines)
0 (Reactive Oxygen Species)
تواريخ الأحداث: Date Created: 20220423 Date Completed: 20220715 Latest Revision: 20231105
رمز التحديث: 20231105
مُعرف محوري في PubMed: PMC9088312
DOI: 10.1002/jcp.30755
PMID: 35460571
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4652
DOI:10.1002/jcp.30755