دورية أكاديمية
أعرض في EDS

Impact of obesity on the molecular response to a single bout of exercise in a preliminary human cohort.

التفاصيل البيبلوغرافية
العنوان: Impact of obesity on the molecular response to a single bout of exercise in a preliminary human cohort.
المؤلفون: Vanderboom P; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA., Zhang X; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.; Department of Geriatrics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, China., Hart CR; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.; Air Force Research Laboratory, 711th Human Performance Wing, Wright Patterson Air Force Base, Dayton, Ohio, USA., Kunz HE; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA., Gries KJ; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.; Exercise and Sports Science, College of Health Professions, Marian University, Indianapolis, Indiana, USA., Heppelmann CJ; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA., Liu Y; Department of Quantitative Health Sciences, Mayo Clinic, Rochester, Minnesota, USA., Dasari S; Department of Quantitative Health Sciences, Mayo Clinic, Rochester, Minnesota, USA., Lanza IR; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.
المصدر: Obesity (Silver Spring, Md.) [Obesity (Silver Spring)] 2022 May; Vol. 30 (5), pp. 1091-1104.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101264860 Publication Model: Print Cited Medium: Internet ISSN: 1930-739X (Electronic) Linking ISSN: 19307381 NLM ISO Abbreviation: Obesity (Silver Spring) Subsets: MEDLINE
أسماء مطبوعة: Publication: 2013- : Malden, MA : John Wiley & Sons
Original Publication: Silver Spring, MD : NAASO, the Obesity Society, c2006-
مواضيع طبية MeSH: Proteome*/metabolism , Proteomics*, Glycogen/metabolism ; Humans ; Muscle, Skeletal/metabolism ; Obesity/metabolism ; Pilot Projects
مستخلص: Objective: The health benefits of exercise are well documented, but several exercise-response parameters are attenuated in individuals with obesity. The goal of this pilot study was to identify molecular mechanisms that may influence exercise response with obesity.
Methods: A multi-omics comparison of the transcriptome, proteome, and phosphoproteome in muscle from a preliminary cohort of lean individuals (n = 4) and individuals with obesity (n = 4) was performed, before and after a single bout of 30 minutes of unilateral cycling at 70% maximal oxygen uptake (VO 2 peak). Mass spectrometry and RNA sequencing were used to interrogate the proteome, phosphoproteome, and transcriptome from muscle biopsy tissue.
Results: The main findings are that individuals with obesity exhibited transcriptional and proteomic signatures consistent with reduced mitochondrial function, protein synthesis, and glycogen synthesis. Furthermore, individuals with obesity demonstrated markedly different transcriptional, proteomic, and phosphoproteomic responses to exercise, particularly biosynthetic pathways of glycogen synthesis and protein synthesis. Casein kinase II subunit alpha and glycogen synthase kinase-3β signaling was identified as exercise-response pathways that were notably altered by obesity.
Conclusions: Opportunities to enhance exercise responsiveness by targeting specific molecular pathways that are disrupted in skeletal muscle from individuals with obesity await a better understanding of the precise molecular mechanisms that may limit exercise-response pathways in obesity.
(© 2022 The Obesity Society.)
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معلومات مُعتمدة: R01 AG054454 United States AG NIA NIH HHS; T32 AR056950 United States AR NIAMS NIH HHS; UL1 TR002377 United States TR NCATS NIH HHS
سلسلة جزيئية: ClinicalTrials.gov NCT02732509
المشرفين على المادة: 0 (Proteome)
9005-79-2 (Glycogen)
تواريخ الأحداث: Date Created: 20220426 Date Completed: 20220428 Latest Revision: 20230505
رمز التحديث: 20230506
مُعرف محوري في PubMed: PMC9048146
DOI: 10.1002/oby.23419
PMID: 35470975
قاعدة البيانات: MEDLINE
الوصف
تدمد:1930-739X
DOI:10.1002/oby.23419