دورية أكاديمية
أعرض في EDS

Multiple sclerosis patients have an altered gut mycobiome and increased fungal to bacterial richness.

التفاصيل البيبلوغرافية
العنوان: Multiple sclerosis patients have an altered gut mycobiome and increased fungal to bacterial richness.
المؤلفون: Yadav M; Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States of America.; University of Iowa College of Dentistry, Iowa City, IA, United States of America., Ali S; Carver College of Medicine, University of Iowa, Iowa City, IA, United States of America., Shrode RL; Informatics Graduate Program, University of Iowa, Iowa City, IA, United States of America., Shahi SK; Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States of America., Jensen SN; Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States of America.; Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA, United States of America., Hoang J; College of Nursing University of Iowa, Iowa City, IA, United States of America., Cassidy S; Department of Neurology, University of Iowa Hospitals and Clinics, Iowa City, IA, United States of America., Olalde H; Department of Neurology, University of Iowa Hospitals and Clinics, Iowa City, IA, United States of America., Guseva N; Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States of America., Paullus M; Department of Neurology, University of Iowa Hospitals and Clinics, Iowa City, IA, United States of America., Cherwin C; College of Nursing University of Iowa, Iowa City, IA, United States of America., Wang K; Department of Biostatistics, College of Public Health, University of Iowa, Iowa City, IA, United States of America., Cho T; Department of Neurology, University of Iowa Hospitals and Clinics, Iowa City, IA, United States of America., Kamholz J; Department of Neurology, University of Iowa Hospitals and Clinics, Iowa City, IA, United States of America., Mangalam AK; Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States of America.; Informatics Graduate Program, University of Iowa, Iowa City, IA, United States of America.; Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA, United States of America.; Iowa City VA Health System, Iowa City, IA, United States of America.
المصدر: PloS one [PLoS One] 2022 Apr 26; Vol. 17 (4), pp. e0264556. Date of Electronic Publication: 2022 Apr 26 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Ascomycota*/genetics , Multiple Sclerosis* , Mycobiome*/genetics, Bacteria/genetics ; Dysbiosis/microbiology ; Fungi/genetics ; Humans ; RNA, Ribosomal, 16S/genetics
مستخلص: Trillions of microbes such as bacteria, fungi, and viruses exist in the healthy human gut microbiome. Although gut bacterial dysbiosis has been extensively studied in multiple sclerosis (MS), the significance of the fungal microbiome (mycobiome) is an understudied and neglected part of the intestinal microbiome in MS. The aim of this study was to characterize the gut mycobiome of patients with relapsing-remitting multiple sclerosis (RRMS), compare it to healthy controls, and examine its association with changes in the bacterial microbiome. We characterized and compared the mycobiome of 20 RRMS patients and 33 healthy controls (HC) using Internal Transcribed Spacer 2 (ITS2) and compared mycobiome interactions with the bacterial microbiome using 16S rRNA sequencing. Our results demonstrate an altered mycobiome in RRMS patients compared with HC. RRMS patients showed an increased abundance of Basidiomycota and decreased Ascomycota at the phylum level with an increased abundance of Candida and Epicoccum genera along with a decreased abundance of Saccharomyces compared to HC. We also observed an increased ITS2/16S ratio, altered fungal and bacterial associations, and altered fungal functional profiles in MS patients compared to HC. This study demonstrates that RRMS patients had a distinct mycobiome with associated changes in the bacterial microbiome compared to HC. There is an increased fungal to bacterial ratio as well as more diverse fungal-bacterial interactions in RRMS patients compared to HC. Our study is the first step towards future studies in delineating the mechanisms through which the fungal microbiome can influence MS disease.
Competing Interests: AM is one of the inventors of a technology claiming the use of Prevotella histicola to treat autoimmune diseases. AM received royalties from Mayo Clinic (paid by Evelo Biosciences). MY, SA, RS, SS, SJ, JH, SC, HO, NG, MP, CC, KW, TC, JK declare no commercial or financial relationships that could be a potential conflict of interest.
References: PLoS One. 2014 Jun 16;9(6):e97629. (PMID: 24933453)
FEMS Microbiol Ecol. 2009 Aug;69(2):231-42. (PMID: 19508502)
Nature. 2012 May 09;486(7402):222-7. (PMID: 22699611)
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6. (PMID: 23193283)
J Genet Eng Biotechnol. 2017 Jun;15(1):197-210. (PMID: 30647656)
Virulence. 2017 Apr 3;8(3):352-358. (PMID: 27736307)
J Lipid Res. 2013 Sep;54(9):2325-40. (PMID: 23821742)
Sci Rep. 2019 May 6;9(1):6923. (PMID: 31061496)
Cell Rep. 2017 Aug 8;20(6):1269-1277. (PMID: 28793252)
Sci Rep. 2020 Jun 15;10(1):9624. (PMID: 32541680)
Gut. 2017 Jun;66(6):1039-1048. (PMID: 26843508)
Neurotherapeutics. 2018 Jan;15(1):109-125. (PMID: 29204955)
J Nutr. 2011 May;141(5):883-9. (PMID: 21430242)
Cell Host Microbe. 2017 Aug 9;22(2):156-165. (PMID: 28799901)
FEMS Microbiol Rev. 2017 Jul 1;41(4):479-511. (PMID: 28430946)
Sci Adv. 2017 Jul 12;3(7):e1700492. (PMID: 28706993)
Nat Methods. 2016 Jul;13(7):581-3. (PMID: 27214047)
Neurol Neuroimmunol Neuroinflamm. 2020 Mar 2;7(3):. (PMID: 32123045)
mSystems. 2019 Mar 26;4(2):. (PMID: 30944880)
Nat Commun. 2016 Jun 28;7:12015. (PMID: 27352007)
Front Microbiol. 2017 Jun 23;8:1162. (PMID: 28690602)
Annu Rev Pathol. 2017 Jan 24;12:359-385. (PMID: 28068483)
J Dent Res. 2005 Nov;84(11):966-77. (PMID: 16246925)
J Immunol. 2004 Mar 1;172(5):2731-8. (PMID: 14978070)
Nature. 2010 Mar 4;464(7285):59-65. (PMID: 20203603)
Science. 2012 Jun 8;336(6086):1314-7. (PMID: 22674328)
PLoS One. 2015 Sep 14;10(9):e0137429. (PMID: 26367776)
Nucleic Acids Res. 2019 Jan 8;47(D1):D259-D264. (PMID: 30371820)
Indian J Rheumatol. 2021 Mar;16(1):57-72. (PMID: 34531642)
J Vis Exp. 2019 Oct 15;(152):. (PMID: 31680682)
Sci Rep. 2016 Jun 27;6:28484. (PMID: 27346372)
Biol Rev Camb Philos Soc. 2020 Apr;95(2):409-433. (PMID: 31763752)
FEBS Lett. 2014 Nov 17;588(22):4214-22. (PMID: 25286403)
Gut. 2016 Feb;65(2):238-48. (PMID: 25567118)
Cell Host Microbe. 2016 Jun 8;19(6):865-73. (PMID: 27237365)
EBioMedicine. 2021 Sep;71:103557. (PMID: 34455391)
Microb Ecol. 2018 May;75(4):821-829. (PMID: 29110065)
PLoS One. 2012;7(7):e40648. (PMID: 22848391)
Sci Rep. 2013;3:2191. (PMID: 23846617)
Semin Immunopathol. 2015 Mar;37(2):107-16. (PMID: 25527294)
Science. 2018 Jan 12;359(6372):232-236. (PMID: 29326275)
J Nutr. 1972 Feb;102(2):269-81. (PMID: 4332855)
Cell Host Microbe. 2020 May 13;27(5):809-822.e6. (PMID: 32209432)
Immunity. 2016 Feb 16;44(2):330-42. (PMID: 26885858)
Antimicrob Agents Chemother. 1993 Jan;37(1):51-3. (PMID: 8431017)
Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):10713-10718. (PMID: 28893978)
PLoS One. 2013 Aug 19;8(8):e71806. (PMID: 23977147)
PLoS Pathog. 2019 Apr 22;15(4):e1007717. (PMID: 31009520)
Eur J Clin Microbiol Infect Dis. 2017 Dec;36(12):2457-2468. (PMID: 28821976)
Nature. 2011 May 12;473(7346):174-80. (PMID: 21508958)
Front Microbiol. 2018 Sep 19;9:2208. (PMID: 30283425)
Pathogens. 2019 May 21;8(2):. (PMID: 31117285)
معلومات مُعتمدة: I01 CX002212 United States CX CSRD VA; P30 ES005605 United States ES NIEHS NIH HHS; R01 AI137075 United States AI NIAID NIH HHS; T90 DE023520 United States DE NIDCR NIH HHS
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20220426 Date Completed: 20220428 Latest Revision: 20240513
رمز التحديث: 20240513
مُعرف محوري في PubMed: PMC9041819
DOI: 10.1371/journal.pone.0264556
PMID: 35472144
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0264556