Quercetin inhibits neutrophil extracellular traps release and their cytotoxic effects on A549 cells, as well the release and enzymatic activity of elastase and myeloperoxidase.

التفاصيل البيبلوغرافية
العنوان:	Quercetin inhibits neutrophil extracellular traps release and their cytotoxic effects on A549 cells, as well the release and enzymatic activity of elastase and myeloperoxidase.
المؤلفون:	Pereira GS; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil., Percebom I; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil., Mendes S; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil., Souza PSS; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil.; Universidade Estadual Paulista - UNESP, Instituto de Biociências, Letras e Ciências Exatas, Programa de Pós-Graduação em Microbiologia, São José do Rio Preto, SP, Brasil., Diniz LFA; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil.; Universidade Estadual Paulista - UNESP, Instituto de Biociências, Letras e Ciências Exatas, Programa de Pós-Graduação em Microbiologia, São José do Rio Preto, SP, Brasil., Costa MF; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil.; Universidade Estadual Paulista - UNESP, Instituto de Biociências, Letras e Ciências Exatas, Programa de Pós-Graduação em Microbiologia, São José do Rio Preto, SP, Brasil., Lopes BRP; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil.; Universidade Estadual Paulista - UNESP, Instituto de Biociências, Letras e Ciências Exatas, Programa de Pós-Graduação em Microbiologia, São José do Rio Preto, SP, Brasil., Toledo KA; Universidade Estadual Paulista - UNESP, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil.; Universidade Estadual Paulista - UNESP, Instituto de Biociências, Letras e Ciências Exatas, Programa de Pós-Graduação em Microbiologia, São José do Rio Preto, SP, Brasil.
المصدر:	Brazilian journal of biology = Revista brasleira de biologia [Braz J Biol] 2022 Apr 20; Vol. 84, pp. e252936. Date of Electronic Publication: 2022 Apr 20 (Print Publication: 2022).
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: International Institute of Ecology Country of Publication: Brazil NLM ID: 101129542 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-4375 (Electronic) Linking ISSN: 15196984 NLM ISO Abbreviation: Braz J Biol Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: São Carlos, SP [Brazil] : International Institute of Ecology, [2000]-
مواضيع طبية MeSH:	Extracellular Traps*/metabolism, A549 Cells ; Humans ; Pancreatic Elastase/metabolism ; Peroxidase ; Quercetin/pharmacology ; Tetradecanoylphorbol Acetate/metabolism ; Tetradecanoylphorbol Acetate/pharmacology
مستخلص:	Neutrophil extracellular traps (NETs) were first reported as a microbicidal strategy for activated neutrophils. Through an immunologic response against several stimuli, neutrophils release their DNA together with proteins from granules, nucleus, and cytoplasm (e.g., elastase and myeloperoxidase). To date, NETs have been implicated in tissue damage during intense inflammatory processes, mainly when their release is dependent on oxygen radical generation. Flavonoids are antioxidant and anti-inflammatory agents; of these, quercetin is commonly found in our daily diet. Therefore, quercetin could exert some protective activity against tissue damage induced by NETs. In our in vitro assays, quercetin reduced NETs, myeloperoxidase (MPO), and elastase release from neutrophils stimulated with phorbol 12-myristate 13-acetate (PMA). The activity of these enzymes also decreased in the presence of quercetin. Quercetin also reduced the cytotoxic effect of NETs on alveolar cells (A549 cell line). Further, in silico assays indicated favorable interactions between quercetin and NET proteins (MPO and elastase). Overall, our results demonstrate that quercetin decreases deleterious cellular effects of NETs by reducing their release from activated neutrophils, and diminishing the enzymatic activity of MPO and elastase, possibly through direct interaction.
المشرفين على المادة:	9IKM0I5T1E (Quercetin) EC 1.11.1.7 (Peroxidase) EC 3.4.21.36 (Pancreatic Elastase) NI40JAQ945 (Tetradecanoylphorbol Acetate)
تواريخ الأحداث:	Date Created: 20220427 Date Completed: 20220429 Latest Revision: 20220429
رمز التحديث:	20221213
DOI:	10.1590/1519-6984.252936
PMID:	35475990
قاعدة البيانات:	MEDLINE

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تدمد:	1678-4375
DOI:	10.1590/1519-6984.252936