دورية أكاديمية
Structure based innovative approach to analyze aptaprobe-GPC3 complexes in hepatocellular carcinoma.
العنوان: | Structure based innovative approach to analyze aptaprobe-GPC3 complexes in hepatocellular carcinoma. |
---|---|
المؤلفون: | Shin WR; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Park DY; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Kim JH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Lee JP; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Thai NQ; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Oh IH; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Sekhon SS; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Choi W; Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea., Kim SY; College of Pharmacy, Wonkwang University, Shinyoung-dong 344-2, Iksan, Jeonbuk, 570-749, Republic of Korea., Cho BK; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Kim SC; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Min J; Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea. jihomin@jbnu.ac.kr., Ahn JY; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea. jyahn@chungbuk.ac.kr., Kim YH; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea. kyh@chungbuk.ac.kr. |
المصدر: | Journal of nanobiotechnology [J Nanobiotechnology] 2022 Apr 27; Vol. 20 (1), pp. 204. Date of Electronic Publication: 2022 Apr 27. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central, 2003- |
مواضيع طبية MeSH: | Carcinoma, Hepatocellular*/pathology , Liver Neoplasms*/pathology, Glypicans/metabolism ; Humans |
مستخلص: | Background: Glypican-3 (GPC3), a membrane-bound heparan sulfate proteoglycan, is a biomarker of hepatocellular carcinoma (HCC) progression. Aptamers specifically binding to target biomolecules have recently emerged as clinical disease diagnosis targets. Here, we describe 3D structure-based aptaprobe platforms for detecting GPC3, such as aptablotting, aptaprobe-based sandwich assay (ALISA), and aptaprobe-based imaging analysis. Results: For preparing the aptaprobe-GPC3 platforms, we obtained 12 high affinity aptamer candidates (GPC3_1 to GPC3_12) that specifically bind to target GPC3 molecules. Structure-based molecular interactions identified distinct aptatopic residues responsible for binding to the paratopic nucleotide sequences (nt-paratope) of GPC3 aptaprobes. Sandwichable and overlapped aptaprobes were selected through structural analysis. The aptaprobe specificity for using in HCC diagnostics were verified through Aptablotting and ALISA. Moreover, aptaprobe-based imaging showed that the binding property of GPC3_3 and their GPC3 specificity were maintained in HCC xenograft models, which may indicate a new HCC imaging diagnosis. Conclusion: Aptaprobe has the potential to be used as an affinity reagent to detect the target in vivo and in vitro diagnosing system. (© 2022. The Author(s).) |
References: | World J Gastroenterol. 2018 Jan 28;24(4):494-503. (PMID: 29398870) J Pharm Sci. 2008 Jan;97(1):237-50. (PMID: 17721944) ACS Synth Biol. 2020 Jan 17;9(1):43-52. (PMID: 31774997) Bioorg Med Chem Lett. 2010 Jun 1;20(11):3322-5. (PMID: 20457520) J Gastroenterol Hepatol. 2010 Jan;25(1):129-37. (PMID: 19793164) Cancers (Basel). 2019 Sep 10;11(9):. (PMID: 31510063) Sci Rep. 2019 Oct 29;9(1):15494. (PMID: 31664144) Int J Cancer. 2003 Feb 10;103(4):455-65. (PMID: 12478660) Proc Natl Acad Sci U S A. 2021 Jul 20;118(29):. (PMID: 34253615) J Biomed Nanotechnol. 2018 Nov 1;14(11):1992-2002. (PMID: 30165934) Front Oncol. 2019 Aug 02;9:708. (PMID: 31428581) J Cancer. 2020 Feb 3;11(8):2008-2021. (PMID: 32127929) Cancer. 1980 Apr 15;45(8):2166-8. (PMID: 6154525) PLoS One. 2017 Feb 8;12(2):e0170975. (PMID: 28178278) Biosens Bioelectron. 2015 Jun 15;68:272-280. (PMID: 25590973) Development. 2015 Oct 15;142(20):3456-67. (PMID: 26487777) Hepatol Int. 2019 Nov;13(6):688-694. (PMID: 31701393) Clin Gastroenterol Hepatol. 2019 Aug;17(9):1744-1755.e11. (PMID: 31077823) Proc Natl Acad Sci U S A. 2021 May 4;118(18):. (PMID: 33911033) Nanoscale. 2017 Jun 8;9(22):7464-7475. (PMID: 28530298) World J Gastroenterol. 2010 Sep 21;16(35):4410-5. (PMID: 20845507) Trends Cancer. 2018 Nov;4(11):741-754. (PMID: 30352677) Sci Rep. 2017 Jan 31;7:41695. (PMID: 28139708) Front Mol Biosci. 2018 May 14;5:41. (PMID: 29868605) Hepatology. 2019 Oct;70(4):1231-1245. (PMID: 30963603) J Clin Gastroenterol. 2010 Apr;44(4):239-45. (PMID: 20216082) Br J Cancer. 2021 Jun;124(12):1988-1996. (PMID: 33824478) Brief Bioinform. 2016 Jan;17(1):117-31. (PMID: 25971595) Sci Rep. 2015 Jun 03;5:10897. (PMID: 26039737) Biosensors (Basel). 2020 Aug 12;10(8):. (PMID: 32806662) Nat Immunol. 2018 Mar;19(3):222-232. (PMID: 29379119) Nanoscale. 2013 Jul 7;5(13):5930-9. (PMID: 23702968) Science. 1990 Aug 3;249(4968):505-10. (PMID: 2200121) J Nucl Med. 2006 Apr;47(4):668-78. (PMID: 16595502) J Am Chem Soc. 2017 Oct 11;139(40):13977-13980. (PMID: 28938065) Mol Immunol. 2021 Mar;131:13-22. (PMID: 33453658) Nucleic Acids Res. 2003 Jul 1;31(13):3406-15. (PMID: 12824337) Mol Pharm. 2021 May 3;18(5):2082-2090. (PMID: 33797932) Prostate Int. 2016 Sep;4(3):118-24. (PMID: 27689070) Biomater Sci. 2018 Dec 18;7(1):159-167. (PMID: 30417190) PLoS One. 2010 Aug 16;5(8):e12029. (PMID: 20808434) Front Chem. 2020 Oct 29;8:570528. (PMID: 33195056) Cell Signal. 2021 Jan;77:109822. (PMID: 33152440) Bioinformatics. 2011 Oct 15;27(20):2915-6. (PMID: 21873642) Mol Ther Nucleic Acids. 2016 Oct 18;5(10):e376. (PMID: 27754489) Bioact Mater. 2021 Oct 15;12:278-291. (PMID: 35310383) Sci Rep. 2018 Feb 1;8(1):2150. (PMID: 29391595) Eur J Cancer. 2011 Feb;47(3):333-8. (PMID: 21112773) Biochimie. 2018 Nov;154:132-155. (PMID: 30193856) Hepatology. 2019 May;69(5):1983-1994. (PMID: 30153338) Bio Protoc. 2018 May 20;8(10):e2850. (PMID: 34285967) Biochemistry. 2004 Sep 7;43(35):11248-54. (PMID: 15366934) ACS Comb Sci. 2018 May 14;20(5):261-268. (PMID: 29553704) Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17099-103. (PMID: 25404344) Liver Int. 2019 Dec;39(12):2214-2229. (PMID: 31436873) J Chem Inf Model. 2011 Oct 24;51(10):2778-86. (PMID: 21919503) Mod Pathol. 2005 Dec;18(12):1591-8. (PMID: 15920546) Arch Med Res. 2014 Oct;45(7):580-8. (PMID: 25446613) Sci Adv. 2016 Mar 25;2(3):e1501240. (PMID: 27051863) Expert Rev Gastroenterol Hepatol. 2017 Apr;11(4):277-279. (PMID: 28162003) J Cell Biol. 2011 Feb 21;192(4):691-704. (PMID: 21339334) J Clin Gastroenterol. 2013 Jul;47 Suppl:S2-6. (PMID: 23632345) Nat Mater. 2017 Nov;16(11):1155-1161. (PMID: 29035356) Nat Commun. 2017 Nov 9;8(1):1390. (PMID: 29123088) |
معلومات مُعتمدة: | NRF-2020R1A2C1009463 National Research Foundation of Korea; 2020R1A6A1A06046235 National Research Foundation of Korea; MAFRA_321108-04 Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries |
فهرسة مساهمة: | Keywords: ALISA; Aptablotting; Aptaprobe; Aptaprobe-based imaging; Cancer diagnosis; Glypican-3; Hepatocellular carcinoma; Structure-based molecular interaction |
المشرفين على المادة: | 0 (GPC3 protein, human) 0 (Glypicans) |
تواريخ الأحداث: | Date Created: 20220428 Date Completed: 20220429 Latest Revision: 20220716 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC9044640 |
DOI: | 10.1186/s12951-022-01391-z |
PMID: | 35477501 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1477-3155 |
---|---|
DOI: | 10.1186/s12951-022-01391-z |