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Structure based innovative approach to analyze aptaprobe-GPC3 complexes in hepatocellular carcinoma.

التفاصيل البيبلوغرافية
العنوان: Structure based innovative approach to analyze aptaprobe-GPC3 complexes in hepatocellular carcinoma.
المؤلفون: Shin WR; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Park DY; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Kim JH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Lee JP; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Thai NQ; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Oh IH; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Sekhon SS; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Choi W; Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea., Kim SY; College of Pharmacy, Wonkwang University, Shinyoung-dong 344-2, Iksan, Jeonbuk, 570-749, Republic of Korea., Cho BK; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Kim SC; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Min J; Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea. jihomin@jbnu.ac.kr., Ahn JY; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea. jyahn@chungbuk.ac.kr., Kim YH; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea. kyh@chungbuk.ac.kr.
المصدر: Journal of nanobiotechnology [J Nanobiotechnology] 2022 Apr 27; Vol. 20 (1), pp. 204. Date of Electronic Publication: 2022 Apr 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Carcinoma, Hepatocellular*/pathology , Liver Neoplasms*/pathology, Glypicans/metabolism ; Humans
مستخلص: Background: Glypican-3 (GPC3), a membrane-bound heparan sulfate proteoglycan, is a biomarker of hepatocellular carcinoma (HCC) progression. Aptamers specifically binding to target biomolecules have recently emerged as clinical disease diagnosis targets. Here, we describe 3D structure-based aptaprobe platforms for detecting GPC3, such as aptablotting, aptaprobe-based sandwich assay (ALISA), and aptaprobe-based imaging analysis.
Results: For preparing the aptaprobe-GPC3 platforms, we obtained 12 high affinity aptamer candidates (GPC3_1 to GPC3_12) that specifically bind to target GPC3 molecules. Structure-based molecular interactions identified distinct aptatopic residues responsible for binding to the paratopic nucleotide sequences (nt-paratope) of GPC3 aptaprobes. Sandwichable and overlapped aptaprobes were selected through structural analysis. The aptaprobe specificity for using in HCC diagnostics were verified through Aptablotting and ALISA. Moreover, aptaprobe-based imaging showed that the binding property of GPC3_3 and their GPC3 specificity were maintained in HCC xenograft models, which may indicate a new HCC imaging diagnosis.
Conclusion: Aptaprobe has the potential to be used as an affinity reagent to detect the target in vivo and in vitro diagnosing system.
(© 2022. The Author(s).)
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معلومات مُعتمدة: NRF-2020R1A2C1009463 National Research Foundation of Korea; 2020R1A6A1A06046235 National Research Foundation of Korea; MAFRA_321108-04 Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries
فهرسة مساهمة: Keywords: ALISA; Aptablotting; Aptaprobe; Aptaprobe-based imaging; Cancer diagnosis; Glypican-3; Hepatocellular carcinoma; Structure-based molecular interaction
المشرفين على المادة: 0 (GPC3 protein, human)
0 (Glypicans)
تواريخ الأحداث: Date Created: 20220428 Date Completed: 20220429 Latest Revision: 20220716
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9044640
DOI: 10.1186/s12951-022-01391-z
PMID: 35477501
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-3155
DOI:10.1186/s12951-022-01391-z