دورية أكاديمية
Characterization of mitochondrial 12S rRNA gene of yellow-striped chevrotain ( Moschiola kathygre ) and white-spotted chevrotain ( Moschiola meminna ) and development of a PCR-RFLP marker for the unambiguous identification of the species.
| العنوان: | Characterization of mitochondrial 12S rRNA gene of yellow-striped chevrotain ( Moschiola kathygre ) and white-spotted chevrotain ( Moschiola meminna ) and development of a PCR-RFLP marker for the unambiguous identification of the species. |
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| المؤلفون: | Sugumar T; Postgraduate Institute of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka., Weerasekera DS; Postgraduate Institute of Science, University of Peradeniya, Peradeniya, Sri Lanka., Ranawana KB; Department of Zoology, Faculty of Science, University of Peradeniya, Peradeniya, Sri Lanka., Jayawardana NU; Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka. |
| المصدر: | Animal biotechnology [Anim Biotechnol] 2023 Dec; Vol. 34 (7), pp. 2065-2072. Date of Electronic Publication: 2022 May 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9011409 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2378 (Electronic) Linking ISSN: 10495398 NLM ISO Abbreviation: Anim Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Abingdon, Oxford : Taylor & Francis Original Publication: New York, N.Y. : Marcel Dekker, c1990- |
| مواضيع طبية MeSH: | Genes, Mitochondrial* , Ruminants*, Animals ; Polymorphism, Restriction Fragment Length ; Phylogeny ; Genes, rRNA ; Polymerase Chain Reaction |
| مستخلص: | Tragulids hold a significant place in the evolutionary history of mammals since they represent the basal branch of ruminants. Only three genera of tragulids are being extant to date such as Tragulus , Hyemoschus and Moschiola . In the genus Moschiola , Sri Lankan chevrotains ( Moschiola meminna and Moschiola kathygre ) are endemic to Sri Lanka while Moschiola indica is present in India. Sri Lankan chevrotains lack information on their population structure, distribution and molecular evidence on species identification. This leads to possible threats including habitat destruction, poaching and illegal hunting and trading under different names. In this study, genomic DNA from hair follicles was isolated from M. meminna and M. kathygre and 12S rRNA mitochondrial gene was amplified using universal primers. The PCR products were sequenced and phylogenetic analysis was done on Sri Lankan chevrotains for the first time. The sequences of Sri Lankan chevrotains share 99.7% similarity as they differ only in a single InDel present in M. meminna . In silico analysis of 12S rRNA region revealed that PCR-RFLP approach can be used to differentiate the Sri Lankan chevrotains from Indian chevrotain using the restriction enzymes; Rsa I , Sca I , Hinf I and Hind III . M. kathygre and M. meminna can be differentiated from each other by using Dra I . |
| فهرسة مساهمة: | Keywords: PCR-RFLP; Sri Lankan chevrotains; Tragulids; ruminants |
| المشرفين على المادة: | 0 (RNA, ribosomal, 12S) |
| تواريخ الأحداث: | Date Created: 20220503 Date Completed: 20231023 Latest Revision: 20231023 |
| رمز التحديث: | 20231023 |
| DOI: | 10.1080/10495398.2022.2070183 |
| PMID: | 35504302 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1532-2378 |
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| DOI: | 10.1080/10495398.2022.2070183 |