دورية أكاديمية
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Identification of Key Pathways Associated With Residual Feed Intake of Beef Cattle Based on Whole Blood Transcriptome Data Analyzed Using Gene Set Enrichment Analysis.

التفاصيل البيبلوغرافية
العنوان: Identification of Key Pathways Associated With Residual Feed Intake of Beef Cattle Based on Whole Blood Transcriptome Data Analyzed Using Gene Set Enrichment Analysis.
المؤلفون: Taiwo GA; Division of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United States., Idowu M; Division of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United States., Denvir J; Department of Medicine, Surgery, and Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, United States., Cervantes AP; Agricultural Research Station, Fort Valley State University, Fort Valley, GA, United States., Ogunade IM; Division of Animal and Nutritional Science, West Virginia University, Morgantown, WV, United States.
المصدر: Frontiers in veterinary science [Front Vet Sci] 2022 Apr 18; Vol. 9, pp. 848027. Date of Electronic Publication: 2022 Apr 18 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2014]-
مستخلص: We applied whole blood transcriptome analysis and gene set enrichment analysis to identify pathways associated with divergent selection for low or high RFI in beef cattle. A group of 56 crossbred beef steers (average BW = 261 ± 18.5 kg) were adapted to a high-forage total mixed ration in a confinement dry lot equipped with GrowSafe intake nodes for period of 49 d to determine their residual feed intake (RFI). After RFI determination, whole blood samples were collected from beef steers with the lowest RFI (most efficient; low-RFI; n = 8) and highest RFI (least efficient; high-RFI; n = 8). Prior to RNA extraction, whole blood samples collected were composited for each steer. Sequencing was performed on an Illumina NextSeq2000 equipped with a P3 flow. Gene set enrichment analysis (GSEA) was used to analyze differentially expressed gene sets and pathways between the two groups of steers. Results of GSEA revealed pathways associated with metabolism of proteins, cellular responses to external stimuli, stress, and heat stress were differentially inhibited (false discovery rate (FDR) < 0.05) in high-RFI compared to low-RFI beef cattle, while pathways associated with binding and uptake of ligands by scavenger receptors, scavenging of heme from plasma, and erythrocytes release/take up oxygen were differentially enriched (FDR < 0.05) in high-RFI, relative to low-RFI beef cattle. Taken together, our results revealed that beef steers divergently selected for low or high RFI revealed differential expressions of genes related to protein metabolism and stress responsiveness.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Taiwo, Idowu, Denvir, Cervantes and Ogunade.)
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فهرسة مساهمة: Keywords: cellular response; feed efficiency; heat stress; oxidative stress; protein metabolism
تواريخ الأحداث: Date Created: 20220506 Latest Revision: 20220716
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC9062580
DOI: 10.3389/fvets.2022.848027
PMID: 35518641
قاعدة البيانات: MEDLINE
الوصف
تدمد:2297-1769
DOI:10.3389/fvets.2022.848027