دورية أكاديمية
Inhibition of the Vancomycin Resistance in Staphylococcus aureus in Egypt Using Silver Nanoparticles.
| العنوان: | Inhibition of the Vancomycin Resistance in Staphylococcus aureus in Egypt Using Silver Nanoparticles. |
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| المؤلفون: | Salah NM; Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.; Department of Microbiology and Immunology, Faculty of Pharmacy, Menoufia University, Shebin Elkom, Menoufia, Egypt., Saafan AE; Department of Microbiology and Immunology, Faculty of Pharmacy, Menoufia University, Shebin Elkom, Menoufia, Egypt., Salem EH; Department of Microbiology and Immunology, Faculty of Medicine, Menoufia, Egypt., El Rabey HA; Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.; Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia., Alsieni MA; Pharmacology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia University, Shebin Elkom, Egypt., Alatawi FA; Department of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia., Alalawy AI; Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia., Abeer Mohammed AB; Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. |
| المصدر: | BioMed research international [Biomed Res Int] 2022 Apr 30; Vol. 2022, pp. 7380147. Date of Electronic Publication: 2022 Apr 30 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Hindawi Pub. Co Country of Publication: United States NLM ID: 101600173 Publication Model: eCollection Cited Medium: Internet ISSN: 2314-6141 (Electronic) NLM ISO Abbreviation: Biomed Res Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Hindawi Pub. Co. |
| مواضيع طبية MeSH: | Metal Nanoparticles* , Methicillin-Resistant Staphylococcus aureus* , Staphylococcal Infections*/epidemiology, Anti-Bacterial Agents/pharmacology ; Egypt ; Humans ; Microbial Sensitivity Tests ; Silver/pharmacology ; Staphylococcus aureus ; Vancomycin/pharmacology ; Vancomycin Resistance/genetics |
| مستخلص: | Staphylococcus aureus is a major human pathogen that is sometimes resistant to vancomycin. In this study, the prevalence of vancomycin-resistant Staphylococcus aureus (VRSA) was studied. 100 isolates of S . aureus were identified based on biochemical and molecular evidence. The antibiotic susceptibility of the studied isolates was tested against 13 antibiotics by the disc diffusion method that showed 24 vancomycin-resistant isolates. The minimum inhibitory concentrations (MICs) were estimated by the agar dilution method to determine vancomycin intermediate-resistant S . aureus (VISA) and VRSA. The resistance gene cluster ( vanA , vanR , vanH , and vanY ) was amplified by PCR and then sequenced. Amplification of vanA and vanR genes showed that they are present in 21.4% and 14.3% of VRSA isolates, respectively, whereas none of the studied genes has been detected in VISA strains. A significant antimicrobial effect toward VRSA isolates using silver nanoparticles (AgNPs) synthesized from S . aureus and rosemary leaves was recorded. This study confirmed the existence of VRSA strains in Egypt. Furthermore, the use of silver nanoparticles inhibits these vancomycin-resistant S . aureus strains in vitro . Competing Interests: None of the authors has a competing interest. (Copyright © 2022 Nouran M. Salah et al.) |
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| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 3M4G523W1G (Silver) 6Q205EH1VU (Vancomycin) |
| تواريخ الأحداث: | Date Created: 20220510 Date Completed: 20220511 Latest Revision: 20220716 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9078785 |
| DOI: | 10.1155/2022/7380147 |
| PMID: | 35535039 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2314-6141 |
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| DOI: | 10.1155/2022/7380147 |