دورية أكاديمية
أعرض في EDS

A widespread family of WYL-domain transcriptional regulators co-localizes with diverse phage defence systems and islands.

التفاصيل البيبلوغرافية
العنوان: A widespread family of WYL-domain transcriptional regulators co-localizes with diverse phage defence systems and islands.
المؤلفون: Picton DM; Department of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, UK., Harling-Lee JD; Department of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, UK.; The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Edinburgh EH25 9RG, UK., Duffner SJ; Department of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, UK., Went SC; Department of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, UK., Morgan RD; New England Biolabs, 240 County Road, Ipswich, MA 01938, USA., Hinton JCD; Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZB, UK., Blower TR; Department of Biosciences, Durham University, Stockton Road, Durham DH1 3LE, UK.
المصدر: Nucleic acids research [Nucleic Acids Res] 2022 May 20; Vol. 50 (9), pp. 5191-5207.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Bacteria*/genetics , Bacteria*/metabolism , Bacteria*/virology , Transcription Factors*/genetics , Transcription Factors*/metabolism, Bacteriophages/genetics ; Genomic Islands/genetics ; Plasmids
مستخلص: Bacteria are under constant assault by bacteriophages and other mobile genetic elements. As a result, bacteria have evolved a multitude of systems that protect from attack. Genes encoding bacterial defence mechanisms can be clustered into 'defence islands', providing a potentially synergistic level of protection against a wider range of assailants. However, there is a comparative paucity of information on how expression of these defence systems is controlled. Here, we functionally characterize a transcriptional regulator, BrxR, encoded within a recently described phage defence island from a multidrug resistant plasmid of the emerging pathogen Escherichia fergusonii. Using a combination of reporters and electrophoretic mobility shift assays, we discovered that BrxR acts as a repressor. We present the structure of BrxR to 2.15 Å, the first structure of this family of transcription factors, and pinpoint a likely binding site for ligands within the WYL-domain. Bioinformatic analyses demonstrated that BrxR-family homologues are widespread amongst bacteria. About half (48%) of identified BrxR homologues were co-localized with a diverse array of known phage defence systems, either alone or clustered into defence islands. BrxR is a novel regulator that reveals a common mechanism for controlling the expression of the bacterial phage defence arsenal.
(© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; BB/M011186/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; 106914/Z/15/Z United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20220511 Date Completed: 20220524 Latest Revision: 20220716
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9122601
DOI: 10.1093/nar/gkac334
PMID: 35544231
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkac334