دورية أكاديمية
Pannexin 1 drives efficient epithelial repair after tissue injury.
| العنوان: | Pannexin 1 drives efficient epithelial repair after tissue injury. |
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| المؤلفون: | Lucas CD; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.; Queen's Medical Research Institute, University of Edinburgh Centre for Inflammation Research, Edinburgh BioQuarter, Edinburgh, UK.; Institute for Regeneration and Repair, Edinburgh BioQuarter, Edinburgh, UK., Medina CB; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA., Bruton FA; Queen's Medical Research Institute, University of Edinburgh Centre for Inflammation Research, Edinburgh BioQuarter, Edinburgh, UK., Dorward DA; Queen's Medical Research Institute, University of Edinburgh Centre for Inflammation Research, Edinburgh BioQuarter, Edinburgh, UK., Raymond MH; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA., Tufan T; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA., Etchegaray JI; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA., Barron B; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA., Oremek MEM; Queen's Medical Research Institute, University of Edinburgh Centre for Inflammation Research, Edinburgh BioQuarter, Edinburgh, UK., Arandjelovic S; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA., Farber E; Center for Public Health Genomics, University of Virginia, Charlottesville, VA, USA., Onngut-Gumuscu S; Center for Public Health Genomics, University of Virginia, Charlottesville, VA, USA., Ke E; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA., Whyte MKB; Queen's Medical Research Institute, University of Edinburgh Centre for Inflammation Research, Edinburgh BioQuarter, Edinburgh, UK., Rossi AG; Queen's Medical Research Institute, University of Edinburgh Centre for Inflammation Research, Edinburgh BioQuarter, Edinburgh, UK., Ravichandran KS; Center for Cell Clearance, Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.; Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.; VIB Center for Inflammation Research and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.; Division of Immunobiology, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA. |
| المصدر: | Science immunology [Sci Immunol] 2022 May 13; Vol. 7 (71), pp. eabm4032. Date of Electronic Publication: 2022 May 13. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101688624 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2470-9468 (Electronic) Linking ISSN: 24709468 NLM ISO Abbreviation: Sci Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Association for the Advancement of Science, [2016]- |
| مواضيع طبية MeSH: | Connexins*/genetics , Connexins*/metabolism , Epithelial Cells*/cytology , Nerve Tissue Proteins*/genetics , Nerve Tissue Proteins*/metabolism , Wounds and Injuries*, Animals ; Lung/metabolism ; Mice ; Neoplasm Proteins ; Zebrafish |
| مستخلص: | Epithelial tissues such as lung and skin are exposed to the environment and therefore particularly vulnerable to damage during injury or infection. Rapid repair is therefore essential to restore function and organ homeostasis. Dysregulated epithelial tissue repair occurs in several human disease states, yet how individual cell types communicate and interact to coordinate tissue regeneration is incompletely understood. Here, we show that pannexin 1 (Panx1), a cell membrane channel activated by caspases in dying cells, drives efficient epithelial regeneration after tissue injury by regulating injury-induced epithelial proliferation. Lung airway epithelial injury promotes the Panx1-dependent release of factors including ATP, from dying epithelial cells, which regulates macrophage phenotype after injury. This process, in turn, induces a reparative response in tissue macrophages that includes the induction of the soluble mitogen amphiregulin, which promotes injury-induced epithelial proliferation. Analysis of regenerating lung epithelium identified Panx1-dependent induction of Nras and Bcas2 , both of which positively promoted epithelial proliferation and tissue regeneration in vivo. We also established that this role of Panx1 in boosting epithelial repair after injury is conserved between mouse lung and zebrafish tailfin. These data identify a Panx1-mediated communication circuit between epithelial cells and macrophages as a key step in promoting epithelial regeneration after injury. |
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| معلومات مُعتمدة: | 206566/Z/17/Z United Kingdom WT_ Wellcome Trust; P01 HL120840 United States HL NHLBI NIH HHS; R35 GM122542 United States GM NIGMS NIH HHS; T32 GM007055 United States GM NIGMS NIH HHS; 206566 United Kingdom WT_ Wellcome Trust; NC/P002196/1 United Kingdom NC3RS_ National Centre for the Replacement, Refinement and Reduction of Animals in Research; T32 AI007496 United States AI NIAID NIH HHS; United Kingdom BHF_ British Heart Foundation; MR/K013386/1 United Kingdom MRC_ Medical Research Council; United Kingdom WT_ Wellcome Trust |
| المشرفين على المادة: | 0 (BCAS2 protein, mouse) 0 (Connexins) 0 (Neoplasm Proteins) 0 (Nerve Tissue Proteins) 0 (Panx1 protein, mouse) |
| تواريخ الأحداث: | Date Created: 20220513 Date Completed: 20220517 Latest Revision: 20240218 |
| رمز التحديث: | 20240218 |
| مُعرف محوري في PubMed: | PMC7612772 |
| DOI: | 10.1126/sciimmunol.abm4032 |
| PMID: | 35559667 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2470-9468 |
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| DOI: | 10.1126/sciimmunol.abm4032 |