دورية أكاديمية
أعرض في EDS

Isolation and Identification of Staphylococcus aureus from Milk and Milk Products, Associated Factors for Contamination, and Their Antibiogram in Holeta, Central Ethiopia.

التفاصيل البيبلوغرافية
العنوان: Isolation and Identification of Staphylococcus aureus from Milk and Milk Products, Associated Factors for Contamination, and Their Antibiogram in Holeta, Central Ethiopia.
المؤلفون: Gebremedhin EZ; Department of Veterinary Sciences, College of Agriculture and Veterinary Science, Ambo; University, P.O. Box 19, Ambo, Ethiopia., Ararso AB; Department of Microbiology, Holeta Polytechnic College, P.O. Box 11, Oromia, Ethiopia., Borana BM; Department of Veterinary Sciences, College of Agriculture and Veterinary Science, Ambo; University, P.O. Box 19, Ambo, Ethiopia., Kelbesa KA; Department of Veterinary Sciences, College of Agriculture and Veterinary Science, Ambo; University, P.O. Box 19, Ambo, Ethiopia., Tadese ND; Department of Veterinary Laboratory Technology, College of Agriculture and Veterinary Science, Ambo University, P.O. Box 19, Ambo, Ethiopia., Marami LM; Department of Veterinary Laboratory Technology, College of Agriculture and Veterinary Science, Ambo University, P.O. Box 19, Ambo, Ethiopia., Sarba EJ; Department of Veterinary Sciences, College of Agriculture and Veterinary Science, Ambo; University, P.O. Box 19, Ambo, Ethiopia.
المصدر: Veterinary medicine international [Vet Med Int] 2022 May 06; Vol. 2022, pp. 6544705. Date of Electronic Publication: 2022 May 06 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Hindawi Country of Publication: United States NLM ID: 101524203 Publication Model: eCollection Cited Medium: Print ISSN: 2090-8113 (Print) Linking ISSN: 20420048 NLM ISO Abbreviation: Vet Med Int Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2011- : New York : Hindawi
Original Publication: Cairo : SAGE-Hindawi, 2010-
مستخلص: Staphylococcus aureus is a pathogenic bacterium-contaminating milk and milk products causing food poisoning primarily due to its enterotoxins. The study aimed at estimating the prevalence of S. aureus in milk and milk products, assessing potential risk factors for contamination, and determining the load and the antimicrobial susceptibility profile of the isolates. A cross-sectional study design was employed to collect a total of 486 samples, comprising 383 raw milk, 47 bulk tank milk, 29 curd milk (Ergo), and 28 Ethiopian cottage cheese (Ayib) samples. Enumeration, isolation, and identification of S. aureus were carried out following standard microbiological techniques. Antibiogram was performed using 12 antimicrobials following the Kirby-Bauer disc diffusion method. Logistic regression analyses were used to assess the association between the occurrence of S. aureus in milk and milk products and potential risk factors. The overall prevalence of S. aureus was 10.69% (52/486) [95% confidence interval (CI):8.09-13.79%]. The prevalence of S. aureus in raw milk, curd milk, bulk tanks at the farm, bulk tanks at milk collection facilities, and cottage cheese was 8.64%, 24.14%, 14.73%, 23.08%, and 14.29%, respectively. The rate of isolation of S. aureus was significantly high in curd milk than in other types of samples ( P  = 0.010). The study revealed that teat washing (OR: 4.93, 95% CI: 2.06-11.81), use of towel (OR: 12.13, 95% CI: 3.74-39.29), and tick infestations (OR: 4.31, 95% CI: 1.28-14.44) were risk factors associated with the occurrence of S. aureus in milk. About 48.39% of the milk samples assessed had the S. aureus count higher than 10 5  CFU/ml. The highest rate of resistance was observed to ampicillin (95%), amoxicillin (95%), oxacillin (87.5%), and cefotaxime (80%). All isolates are resistant to at least two classes of antimicrobial drugs, while 65.0% of the isolates were found to be multidrug-resistant. The moderate prevalence, high load, and antimicrobial resistance of S. aureus indicate the higher public health risk due to the widespread consumption of raw milk in the area. Good hygienic practices, regular surveillance of antimicrobial resistance, and prudent use of drugs are suggested.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2022 Endrias Zewdu Gebremedhin et al.)
References: J Dairy Sci. 2012 Dec;95(12):7377-83. (PMID: 23040016)
Trop Anim Health Prod. 2019 May;51(4):939-948. (PMID: 30565186)
BMC Microbiol. 2017 Jun 27;17(1):141. (PMID: 28655298)
BMC Microbiol. 2016 Jul 29;16(1):169. (PMID: 27473328)
J Dairy Sci. 2014 Nov;97(11):6907-16. (PMID: 25242420)
Appl Environ Microbiol. 2015 Nov 20;82(3):816-21. (PMID: 26590279)
Vet Med Int. 2019 Jul 28;2019:3063185. (PMID: 31467658)
Lett Appl Microbiol. 2014 Jun;58(6):527-34. (PMID: 24460961)
Trop Anim Health Prod. 2016 Feb;48(2):451-9. (PMID: 26719295)
J Clin Microbiol. 2012 Mar;50(3):688-95. (PMID: 22170937)
Infect Drug Resist. 2021 Apr 19;14:1493-1504. (PMID: 33907427)
Food Microbiol. 2017 Apr;62:141-146. (PMID: 27889140)
Ann Clin Microbiol Antimicrob. 2012 Sep 17;11:26. (PMID: 25927182)
J Dairy Sci. 2016 Dec;99(12):9560-9569. (PMID: 27771092)
J Dairy Sci. 2012 Mar;95(3):1283-300. (PMID: 22365211)
Vet World. 2019;12(2):205-210. (PMID: 31040559)
BMC Infect Dis. 2017 Jun 13;17(1):422. (PMID: 28610560)
FEMS Microbiol Rev. 2012 Jul;36(4):815-36. (PMID: 22091892)
Int J Food Microbiol. 2019 Feb 16;291:35-41. (PMID: 30445283)
J Clin Microbiol. 2008 Nov;46(11):3728-35. (PMID: 18799704)
Int J Food Microbiol. 2017 Sep 1;256:30-35. (PMID: 28582663)
Zoonoses Public Health. 2015 Dec;62(8):637-43. (PMID: 25916167)
Acta Trop. 2017 Dec;176:168-172. (PMID: 28797802)
J Dairy Sci. 2005 Nov;88(11):3810-7. (PMID: 16230686)
Acta Trop. 2018 Jan;177:32-36. (PMID: 28935553)
Braz J Microbiol. 2013 Oct 30;44(2):393-9. (PMID: 24294227)
PLoS One. 2016 May 17;11(5):e0155621. (PMID: 27187065)
J Dairy Sci. 2013 Jan;96(1):219-33. (PMID: 23164233)
BMC Vet Res. 2016 Dec 3;12(1):270. (PMID: 27912754)
تواريخ الأحداث: Date Created: 20220516 Latest Revision: 20220716
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9106507
DOI: 10.1155/2022/6544705
PMID: 35574151
قاعدة البيانات: MEDLINE
الوصف
تدمد:2090-8113
DOI:10.1155/2022/6544705