دورية أكاديمية
Novel Salt-Tolerant Leucine Dehydrogenase from Marine Pseudoalteromonas rubra DSM 6842.
| العنوان: | Novel Salt-Tolerant Leucine Dehydrogenase from Marine Pseudoalteromonas rubra DSM 6842. |
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| المؤلفون: | Chen R; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue East Road, Nanning, 530004, People's Republic of China., Liao YT; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue East Road, Nanning, 530004, People's Republic of China., Gao TT; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue East Road, Nanning, 530004, People's Republic of China., Zhang YM; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue East Road, Nanning, 530004, People's Republic of China., Lu LH; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue East Road, Nanning, 530004, People's Republic of China., Wang CH; College of Light Industry and Food Engineering, Guangxi University, 100 Daxue East Road, Nanning, 530004, People's Republic of China. chwang@gxu.edu.cn. |
| المصدر: | Molecular biotechnology [Mol Biotechnol] 2022 Nov; Vol. 64 (11), pp. 1270-1278. Date of Electronic Publication: 2022 May 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Switzerland NLM ID: 9423533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [Cham] : Springer Original Publication: Totowa, NJ : Humana Press, c1994- |
| مواضيع طبية MeSH: | Escherichia coli*/genetics , Pseudoalteromonas*/genetics, Cloning, Molecular ; Enzyme Stability ; Hydrogen-Ion Concentration ; Leucine/genetics ; Leucine Dehydrogenase ; Recombinant Proteins/genetics ; Sodium Chloride |
| مستخلص: | This study reported the cloning, expression, and characterization of a new salt-tolerant leucine dehydrogenase (PrLeuDH) from Pseudoalteromonas rubra DSM 6842. A codon-optimized 1038 bp gene encoding PrLeuDH was successfully expressed on pET-22b( +) in E. coli BL21(DE3). The purified recombinant PrLeuDH showed a single band of about 38.7 kDa on SDS-PAGE. It exhibited the maximum activity at 40 °C and pH 10.5, while kept high activities in the range of 25-45 °C and pH 9.5-12. The K (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| معلومات مُعتمدة: | No. AD211064 the Guangxi science and technology base and talent special project; No. Bhsfs010-4 the Beihai 13th five year plan |
| فهرسة مساهمة: | Keywords: Alkali resistance; Biocatalyst; Cold-adapted; Leucine dehydrogenase; Pseudoalteromonas rubra; Salt-tolerant; α-ketoacid |
| المشرفين على المادة: | 0 (Recombinant Proteins) 451W47IQ8X (Sodium Chloride) EC 1.4.1.9 (Leucine Dehydrogenase) GMW67QNF9C (Leucine) |
| SCR Organism: | Pseudoalteromonas rubra |
| تواريخ الأحداث: | Date Created: 20220516 Date Completed: 20220929 Latest Revision: 20220929 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s12033-022-00505-0 |
| PMID: | 35578070 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1559-0305 |
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| DOI: | 10.1007/s12033-022-00505-0 |