دورية أكاديمية
Detecting Intact Virus Using Exogenous Oligonucleotide Labels.
| العنوان: | Detecting Intact Virus Using Exogenous Oligonucleotide Labels. |
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| المؤلفون: | Carey TR; UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, California 94720, United States., Kozminsky M; California Institute for Quantitative Biosciences, University of California, Berkeley, 174 Stanley Hall, Berkeley, California 94720, United States., Hall J; Department of Molecular and Cellular Biology, University of California, Berkeley, 3200 Weill Hall, Berkeley, California 94720, United States., Vargas-Zapata V; Department of Molecular and Cellular Biology, University of California, Berkeley, 3200 Weill Hall, Berkeley, California 94720, United States., Geiger K; Department of Molecular and Cellular Biology, University of California, Berkeley, 3200 Weill Hall, Berkeley, California 94720, United States., Coscoy L; Department of Molecular and Cellular Biology, University of California, Berkeley, 3200 Weill Hall, Berkeley, California 94720, United States., Sohn LL; UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, 306 Stanley Hall, Berkeley, California 94720, United States.; Department of Mechanical Engineering, University of California, Berkeley, 5118 Etcheverry Hall, Berkeley, California 94720, United States. |
| المصدر: | Analytical chemistry [Anal Chem] 2022 May 31; Vol. 94 (21), pp. 7619-7627. Date of Electronic Publication: 2022 May 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | COVID-19*/diagnosis , Pandemics*, Humans ; Liposomes ; Oligonucleotides ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics ; Sensitivity and Specificity |
| مستخلص: | The COVID-19 pandemic has revealed how an emerging pathogen can cause a sudden and dramatic increase in demand for viral testing. Testing pooled samples could meet this demand; however, the sensitivity of reverse transcription quantitative polymerase chain reaction (RT-qPCR), the gold standard, significantly decreases with an increasing number of samples pooled. Here, we introduce detection of intact virus by exogenous-nucleotide reaction (DIVER), a method that quantifies intact virus and is robust to sample dilution. As demonstrated using two models of severe acute respiratory syndrome coronavirus 2, DIVER first tags membraned particles with exogenous oligonucleotides, then captures the tagged particles on beads functionalized with a virus-specific capture agent (in this instance, angiotensin-converting enzyme 2), and finally quantifies the oligonucleotide tags using qPCR. Using spike-presenting liposomes and spike-pseudotyped lentivirus, we show that DIVER can detect 1 × 10 5 liposomes and 100 plaque-forming units of lentivirus and can successfully identify positive samples in pooling experiments. Overall, DIVER is well positioned for efficient sample pooling and clinical validation. |
| التعليقات: | Update of: medRxiv. 2021 Mar 26;:. (PMID: 33791715) |
| معلومات مُعتمدة: | F32 CA243354 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Liposomes) 0 (Oligonucleotides) |
| تواريخ الأحداث: | Date Created: 20220518 Date Completed: 20220602 Latest Revision: 20221103 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/acs.analchem.2c00835 |
| PMID: | 35584293 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6882 |
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| DOI: | 10.1021/acs.analchem.2c00835 |