دورية أكاديمية
أعرض في EDS

Nosocomial transmission and rearrangement of large resistance-virulence hybrid plasmids between two bacteremic ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae strains with low fitness cost.

التفاصيل البيبلوغرافية
العنوان: Nosocomial transmission and rearrangement of large resistance-virulence hybrid plasmids between two bacteremic ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae strains with low fitness cost.
المؤلفون: Liao W; Department of Respiratory and Critical Care, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China., Huang QS; Department of Clinical Microbiology, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China., Wei D; Department of Clinical Microbiology, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China., Xiong Z; Department of Respiratory and Critical Care, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China., Du FL; Department of Clinical Microbiology, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China., Xiang TX; Department of Infectious Disease, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China., Zhang S; Department of Geriatrics, Jiangxi kangning Hospital, Nanchang, 330006, PR China., Wan LG; Department of Clinical Microbiology, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China., Zhang W; Department of Respiratory and Critical Care, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China. Electronic address: zhangweiliuxin@163.com., Liu Y; Department of Clinical Microbiology, First Affiliated Hospital of Nanchang University, Nanchang University, Yong wai zheng jie No. 17, Nanchang, 330006, PR China. Electronic address: ly13767160474@sina.com.
المصدر: Microbial pathogenesis [Microb Pathog] 2022 Jul; Vol. 168, pp. 105593. Date of Electronic Publication: 2022 May 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London ; Orlando : Academic Press, c1986-
مواضيع طبية MeSH: Bacteremia* , Carbapenem-Resistant Enterobacteriaceae*/genetics , Cross Infection* , Klebsiella Infections*/epidemiology, Anti-Bacterial Agents/pharmacology ; Carbapenems/pharmacology ; Humans ; Klebsiella pneumoniae ; Plasmids/genetics ; Virulence/genetics ; beta-Lactamases/genetics
مستخلص: Objectives: To characterize nosocomial transmission and rearrangement of the resistance-virulence plasmid between two ST11-K64 carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) strains (JX-CR-hvKP-10 and JX-CR-hvKP-9) with low fitness.
Methods: Phenotypic tests were used to assess the virulence of JX-CR-hvKP-10 and JX-CR-hvKP-9. Whole-genome sequencing was used to analyze JX-CR-hvKP-10 and JX-CR-hvKP-9 chromosomes and plasmids. Fitness and conjugation experiments were also conducted using these two CR-hvKP isolates.
Results: Phenotypic tests indicated that both JX-CR-hvKP-10 and JX-CR-hvKP-9 were multidrug-resistant and hypervirulent K. pneumoniae. Whole-genome sequencing and clinical information demonstrated that the super large resistance-virulence fusion plasmid pJX10-1 formed precisely by the fusion of pJX9-1 and pJX9-2 via the nosocomial transmission. Interestingly pJX9-1 itself was also a classic resistance-virulence fusion plasmid by way of the bla KPC -carrying resistance plasmid and pLVPK-like virulence plasmid. Compared with classic K. pneumoniae ATCC700603, fitness analysis revealed no significant difference in growth was observed between JX-CR-hvKP-10 and JX-CR-hvKP-9.
Conclusion: Nosocomial transmission and rearrangement of a bla KPC -harboring plasmid and a pLVPK-like virulence plasmid with a low fitness cost in ST11 K. pneumoniae enhances drug resistance and virulence simultaneously. Thus, active surveillance of this hybrid plasmid is needed to prevent these efficient resistance-virulence plasmids from disseminating in hospital settings.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Carbapenem resistance; Hypervirulent Klebsiella pneumoniae; KPC-2; Plasmid fusion; pLVPK-like virulence plasmid
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Carbapenems)
EC 3.5.2.6 (beta-Lactamases)
تواريخ الأحداث: Date Created: 20220520 Date Completed: 20220617 Latest Revision: 20220617
رمز التحديث: 20231215
DOI: 10.1016/j.micpath.2022.105593
PMID: 35595177
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-1208
DOI:10.1016/j.micpath.2022.105593