دورية أكاديمية
Ethanol concentration induces production of 3,4-dihydroxyphenylacetic acid and homovanillic acid in mouse brain through activation of monoamine oxidase pathway.
| العنوان: | Ethanol concentration induces production of 3,4-dihydroxyphenylacetic acid and homovanillic acid in mouse brain through activation of monoamine oxidase pathway. |
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| المؤلفون: | Jamal M; Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. Electronic address: mostofa.jamal@kagawa-u.ac.jp., Ito A; Department of Forensic Medicine, Faculty of Medicine, Tokushima University, Japan., Miki T; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Japan., Suzuki S; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Japan., Ohta KI; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Japan., Kinoshita H; Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. |
| المصدر: | Neuroscience letters [Neurosci Lett] 2022 Jun 21; Vol. 782, pp. 136689. Date of Electronic Publication: 2022 May 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Publishers Ireland Country of Publication: Ireland NLM ID: 7600130 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7972 (Electronic) Linking ISSN: 03043940 NLM ISO Abbreviation: Neurosci Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Scientific Publishers Ireland Original Publication: Amsterdam, Elsevier/North-Holland. |
| مواضيع طبية MeSH: | Monoamine Oxidase*/metabolism , Serotonin*/metabolism, 3,4-Dihydroxyphenylacetic Acid/metabolism ; Aldehyde Dehydrogenase, Mitochondrial/metabolism ; Animals ; Brain/metabolism ; Catechol O-Methyltransferase/metabolism ; Clorgyline/metabolism ; Clorgyline/pharmacology ; Ethanol/pharmacology ; Homovanillic Acid/metabolism ; Mice ; Norepinephrine/metabolism ; Tolcapone/metabolism ; Tolcapone/pharmacology |
| مستخلص: | First, we aimed to investigate ex vivo the effects of ethanol (EtOH) on levels of norepinephrine (NE), dopamine (DA), serotonin (5-HT), and their metabolites in the frontal cortex, hippocampus, and striatum of Aldh2-knockout (Aldh2-KO) and wild-type (WT) mice. Animals were treated intraperitoneally with saline (control) or EtOH (1.0, 2.0, or 3.0 g/kg). Brain samples were collected 60 and 120 min after EtOH injection, and monoamines and their metabolites were measured by HPLC-ECD. We found in both WT and Aldh2-KO mice that 3.0 g/kg EtOH increased the levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) and decreased the level of 3-methoxytyramine (3-MT). A 2.0 g/kg dose of EtOH also increased HVA, but there was not a consistent effect within the brain regions of Aldh2-KO and WT mice. There were inconsistent findings of genotype differences in the levels of DA, 5-HT, and their metabolites in the brain regions tested. None of the EtOH doses altered NE, DA, 5-HT, or 5-hydroxyindoleacetic acid contents in any of the brain regions studied. Second, we tested whether EtOH-induced increases in DOPAC and HVA are mediated by increased monoamine oxidase (MAO) or catechol-O-methyltransferase (COMT) activity. To test this, we used the MAO blocker clorgyline (2.0 and 4.0 mg/kg) and the COMT blocker tolcapone (15 and 30 mg/kg) alone or in combination with EtOH (3.0 g/kg). Clorgyline alone increased 3-MT and decreased DOPAC and HVA levels, whereas tolcapone alone increased DOPAC and decreased 3-MT and HVA levels. Surprisingly, the combination of EtOH with clorgyline (4.0 mg/kg) or tolcapone (30 mg/kg) further decreased 3-MT and increased DOPAC and HVA levels, an effect that reversed the inhibitor-induced decreases in HVA. These results suggest that a high concentration of EtOH can accelerate DA metabolism, as evidenced by the increase in DOPAC and HVA, and this effect is likely a consequence of increased degradation of DA by MAO. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Catechol-O-methyltransferase; Ethanol; Monoamine oxidase; Monoamines; Mouse brain |
| المشرفين على المادة: | 102-32-9 (3,4-Dihydroxyphenylacetic Acid) 333DO1RDJY (Serotonin) 3K9958V90M (Ethanol) CIF6334OLY (Tolcapone) EC 1.2.1.3 (Aldehyde Dehydrogenase, Mitochondrial) EC 1.4.3.4 (Monoamine Oxidase) EC 2.1.1.6 (Catechol O-Methyltransferase) LYJ16FZU9Q (Clorgyline) X4W3ENH1CV (Norepinephrine) X77S6GMS36 (Homovanillic Acid) |
| تواريخ الأحداث: | Date Created: 20220522 Date Completed: 20220620 Latest Revision: 20220624 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.neulet.2022.136689 |
| PMID: | 35598694 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7972 |
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| DOI: | 10.1016/j.neulet.2022.136689 |