دورية أكاديمية
أعرض في EDS

Generation of a mitochondrial protein compendium in Dictyostelium discoideum .

التفاصيل البيبلوغرافية
العنوان: Generation of a mitochondrial protein compendium in Dictyostelium discoideum .
المؤلفون: Freitas AV; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Herb JT; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Pan M; National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA., Chen Y; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Gucek M; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Jin T; National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA., Xu H; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA.
المصدر: IScience [iScience] 2022 Apr 29; Vol. 25 (5), pp. 104332. Date of Electronic Publication: 2022 Apr 29 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, [2018]-
مستخلص: The social ameba Dictyostelium discoideum has emerged as a powerful model to study mitochondrial genetics and bioenergetics. However, a comprehensive inventory of mitochondrial proteins that is critical to understanding mitochondrial processes has yet to be curated. Here, we utilized high-throughput multiplexed protein quantitation and homology analyses to generate a high-confidence mitochondrial protein compendium consisting of 936 proteins. Our proteomic approach, which utilizes mass spectrometry in combination with mathematical modeling, was validated through mitochondrial targeting sequence prediction and live-cell imaging. Our final compendium consists of 936 proteins. Nearly, a third of D. discoideum mitochondrial proteins do not have homologs in humans, budding yeasts, or an ancestral alphaproteobacteria. Additionally, we leverage our compendium to highlight the complexity of metabolic reprogramming during starvation-induced development. Our compendium lays a foundation to investigate mitochondrial processes that are unique in ameba and to understand the functions of conserved mitochondrial proteins in D. discoideum .
Competing Interests: The authors declare no competing interests.
References: Evolution. 2015 Dec;69(12):3069-81. (PMID: 26514987)
Front Mol Biosci. 2017 Dec 07;4:83. (PMID: 29270408)
BMC Genet. 2008 Mar 21;9:25. (PMID: 18366713)
Plant Cell. 2004 Aug;16(8):2089-103. (PMID: 15269332)
Nature. 2021 Mar;591(7850):471-476. (PMID: 33627869)
Life Sci Alliance. 2019 Sep 30;2(5):. (PMID: 31570514)
J Exp Biol. 2009 Aug;212(Pt 16):2627-34. (PMID: 19648408)
Genome Biol. 2010;11(3):R35. (PMID: 20236529)
Methods Mol Biol. 2013;983:17-37. (PMID: 23494300)
Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):12007-12. (PMID: 9342353)
Cell. 2008 Jul 11;134(1):112-23. (PMID: 18614015)
J Muscle Res Cell Motil. 2002;23(7-8):839-52. (PMID: 12952082)
Experientia. 1990 Dec 1;46(11-12):1117-26. (PMID: 2253709)
Mol Biol Evol. 2014 Mar;31(3):673-82. (PMID: 24336923)
Bioinformatics. 2009 Jan 15;25(2):288-9. (PMID: 19033274)
Int J Genomics. 2013;2013:586498. (PMID: 23998118)
Cell. 2009 Oct 16;139(2):428-39. (PMID: 19837041)
BMC Bioinformatics. 2017 Jun 2;18(1):291. (PMID: 28578698)
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14244-9. (PMID: 9826685)
Proc Natl Acad Sci U S A. 1982 May;79(10):3228-31. (PMID: 6285342)
Nucleic Acids Res. 2021 Jan 8;49(D1):D1541-D1547. (PMID: 33174596)
J Cell Biol. 1986 Sep;103(3):875-86. (PMID: 3745272)
BMC Evol Biol. 2017 Dec 28;17(1):269. (PMID: 29281964)
J Mol Evol. 1995 Jun;40(6):616-21. (PMID: 7643412)
Nat Protoc. 2007;2(6):1317-24. (PMID: 17545968)
Cell Rep. 2017 Jun 27;19(13):2836-2852. (PMID: 28658629)
Mol Biol Evol. 2019 May 1;36(5):1022-1036. (PMID: 30785203)
EMBO Rep. 2017 Oct;18(10):1786-1800. (PMID: 28827470)
EMBO Rep. 2008 Jan;9(1):42-9. (PMID: 18174896)
Plasmid. 2009 Mar;61(2):110-8. (PMID: 19063918)
Expert Rev Proteomics. 2012 Apr;9(2):129-34. (PMID: 22462785)
Nucleic Acids Res. 2021 Jan 8;49(D1):D325-D334. (PMID: 33290552)
Curr Protoc Bioinformatics. 2013 Jun;Chapter 3:3.1.1-3.1.8. (PMID: 23749753)
Nature. 1984 Apr 12-18;308(5960):613-7. (PMID: 6546784)
Mol Gen Genet. 2000 Apr;263(3):514-9. (PMID: 10821186)
J Cell Sci. 2004 Jul 1;117(Pt 15):3141-52. (PMID: 15226392)
FEBS Lett. 2002 Dec 18;532(3):459-64. (PMID: 12482613)
Genes (Basel). 2021 Apr 25;12(5):. (PMID: 33923051)
Curr Genet. 1995 Feb;27(3):249-56. (PMID: 7736610)
RNA Biol. 2013;10(9):1433-8. (PMID: 23635806)
Am J Physiol Cell Physiol. 2011 Jun;300(6):C1280-90. (PMID: 21289287)
Curr Protoc Cell Biol. 2001 May;Chapter 3:Unit 3.4. (PMID: 18228356)
Protein Expr Purif. 2020 Jan;165:105501. (PMID: 31542563)
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. (PMID: 9254694)
Int J Dev Biol. 2019;63(8-9-10):497-508. (PMID: 31840787)
Semin Cell Dev Biol. 2011 Feb;22(1):120-30. (PMID: 21129494)
Anal Chem. 2008 Apr 15;80(8):2921-31. (PMID: 18312001)
Nat Protoc. 2007;2(6):1307-16. (PMID: 17545967)
BMC Bioinformatics. 2009 Dec 15;10:421. (PMID: 20003500)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
Eukaryot Cell. 2006 Aug;5(8):1314-27. (PMID: 16896215)
Biochimie. 2015 Jun;113:93-9. (PMID: 25882680)
Prog Lipid Res. 2011 Oct;50(4):403-10. (PMID: 21801748)
فهرسة مساهمة: Keywords: Microbiology; Microorganism; Omics; Proteomics
تواريخ الأحداث: Date Created: 20220523 Latest Revision: 20230916
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9118663
DOI: 10.1016/j.isci.2022.104332
PMID: 35602934
قاعدة البيانات: MEDLINE
الوصف
تدمد:2589-0042
DOI:10.1016/j.isci.2022.104332