دورية أكاديمية
Generation of a mitochondrial protein compendium in Dictyostelium discoideum .
العنوان: | Generation of a mitochondrial protein compendium in Dictyostelium discoideum . |
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المؤلفون: | Freitas AV; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Herb JT; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Pan M; National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA., Chen Y; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Gucek M; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA., Jin T; National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA., Xu H; National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA. |
المصدر: | IScience [iScience] 2022 Apr 29; Vol. 25 (5), pp. 104332. Date of Electronic Publication: 2022 Apr 29 (Print Publication: 2022). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2018]- |
مستخلص: | The social ameba Dictyostelium discoideum has emerged as a powerful model to study mitochondrial genetics and bioenergetics. However, a comprehensive inventory of mitochondrial proteins that is critical to understanding mitochondrial processes has yet to be curated. Here, we utilized high-throughput multiplexed protein quantitation and homology analyses to generate a high-confidence mitochondrial protein compendium consisting of 936 proteins. Our proteomic approach, which utilizes mass spectrometry in combination with mathematical modeling, was validated through mitochondrial targeting sequence prediction and live-cell imaging. Our final compendium consists of 936 proteins. Nearly, a third of D. discoideum mitochondrial proteins do not have homologs in humans, budding yeasts, or an ancestral alphaproteobacteria. Additionally, we leverage our compendium to highlight the complexity of metabolic reprogramming during starvation-induced development. Our compendium lays a foundation to investigate mitochondrial processes that are unique in ameba and to understand the functions of conserved mitochondrial proteins in D. discoideum . Competing Interests: The authors declare no competing interests. |
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فهرسة مساهمة: | Keywords: Microbiology; Microorganism; Omics; Proteomics |
تواريخ الأحداث: | Date Created: 20220523 Latest Revision: 20230916 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9118663 |
DOI: | 10.1016/j.isci.2022.104332 |
PMID: | 35602934 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2589-0042 |
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DOI: | 10.1016/j.isci.2022.104332 |