دورية أكاديمية
أعرض في EDS

Human tumor necrosis factor alpha affects the egg-laying dynamics and glucose metabolism of Schistosoma mansoni adult worms in vitro.

التفاصيل البيبلوغرافية
العنوان: Human tumor necrosis factor alpha affects the egg-laying dynamics and glucose metabolism of Schistosoma mansoni adult worms in vitro.
المؤلفون: Lopes-Junior EH; Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., Bertevello CR; Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., de Oliveira Silveira G; Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brazil.; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Guedes CB; Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., Rodrigues GD; Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, Brazil., Ribeiro VS; Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., Amaral MS; Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brazil., Kanamura CT; Centro de Patologia, Instituto Adolfo Lutz, São Paulo, Brazil., Pinto PLS; Centro de Parasitologia e Micologia, Instituto Adolfo Lutz, São Paulo, Brazil., Krüger RF; Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, Brazil., Verjovski-Almeida S; Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brazil.; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Oliveira KC; Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil. katia.oliveira@unifesp.br.
المصدر: Parasites & vectors [Parasit Vectors] 2022 May 24; Vol. 15 (1), pp. 176. Date of Electronic Publication: 2022 May 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Schistosoma mansoni* , Schistosomiasis mansoni*/parasitology, Adenosine Triphosphate/pharmacology ; Animals ; Glucose ; Humans ; Lactates/pharmacology ; RNA, Messenger/genetics ; Tumor Necrosis Factor-alpha/genetics
مستخلص: Several studies have described the effects of human tumor necrosis factor alpha (hTNF-α) on Schistosoma mansoni. hTNF-α affects the worm's development, metabolism, egg-laying, gene expression and protein phosphorylation. The available data on the influence of hTNF-α on egg-laying in S. mansoni are controversial, but understanding the mechanism of egg-laying regulation in this species is essential in combating schistosomiasis. We characterized the effects of in vitro treatment of S. mansoni adult worms with different doses of hTNF-α (5, 20 and 40 ng/ml) for 5 days. We explored the effects on egg-laying rate, glucose levels, ATP metabolism, and messenger RNA (mRNA) expression levels of lactate dehydrogenase, glucose transporters and the parasite gene which acts as an hTNF-α receptor, SmTNFR. hTNF-α influenced egg-laying in a time- and dose-dependent manner: at a dose of 40 ng/ml, egg-laying increased on day 2 and decreased on days 3 and 4; at 20 ng/ml, egg-laying decreased on day 3; while at 5 ng/ml, egg-laying decreased on day 4. The total number of eggs produced was not affected by the different treatments, but the egg-laying dynamics were: the median egg-laying time decreased significantly with treatment, and egg developmental stages and size were also affected. At 5 and 20 ng/ml hTNF-α, lactate production diminished on day 3 up to day 5, while glucose uptake increased on day 5. At 40 ng/ml, glucose uptake diminished on day 1 up to day 3, while ATP accumulation was detected on day 5. No significant changes in mRNA expression were detected in any of the treatments. We found that crosstalk involving hTNF-α and parasite signaling plays a role in the fine-scale regulation of the worm's metabolism and physiology, and points to new strategies for disease control.
(© 2022. The Author(s).)
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معلومات مُعتمدة: 2012/50115-6 Fundação de Amparo à Pesquisa do Estado de São Paulo
فهرسة مساهمة: Keywords: Cell signaling; Egg-laying; Glucose metabolism; Host–parasite interaction; Human tumor necrosis factor alpha; Schistosoma mansoni
المشرفين على المادة: 0 (Lactates)
0 (RNA, Messenger)
0 (Tumor Necrosis Factor-alpha)
8L70Q75FXE (Adenosine Triphosphate)
IY9XDZ35W2 (Glucose)
تواريخ الأحداث: Date Created: 20220524 Date Completed: 20220526 Latest Revision: 20220716
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9128126
DOI: 10.1186/s13071-022-05278-8
PMID: 35610661
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-022-05278-8