دورية أكاديمية
أعرض في EDS

Transcriptomic analysis of the chorioallantois in equine premature placental separation.

التفاصيل البيبلوغرافية
العنوان: Transcriptomic analysis of the chorioallantois in equine premature placental separation.
المؤلفون: Murase H; Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.; Equine Science Division, Hidaka Training and Research Center, Japan Racing Association, Hokkaido, Japan., El-Sheikh Ali H; Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.; Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt., Ruby RE; Veterinary Diagnostic Laboratory, University of Kentucky, Lexington, Kentucky, USA., Scoggin KE; Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA., Ball BA; Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, USA.
المصدر: Equine veterinary journal [Equine Vet J] 2023 May; Vol. 55 (3), pp. 405-418. Date of Electronic Publication: 2022 Jun 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 0173320 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2042-3306 (Electronic) Linking ISSN: 04251644 NLM ISO Abbreviation: Equine Vet J Subsets: MEDLINE
أسماء مطبوعة: Publication: <2009- > : Hobokken, NJ : Wiley
Original Publication: Newmarket, Suffolk : Equine Veterinary Journal, Ltd
مواضيع طبية MeSH: Placenta* , Inflammation*/metabolism , Inflammation*/veterinary , Horse Diseases*/etiology, Animals ; Pregnancy ; Horses/genetics ; Female ; Transcriptome ; Retrospective Studies
مستخلص: Background: Equine premature placental separation (PPS) is poorly understood and represents an important risk factor for fetal/neonatal hypoxia.
Objectives: To examine transcriptomic changes in the chorioallantois (CA) from mares with clinical PPS compared with the CA from normal foaling mares. Differential gene expression was determined and gene ontology as well as molecular pathways related to PPS were characterised.
Study Design: Retrospective case: control study.
Methods: CA were collected from Thoroughbred mares with a clinical history of PPS (n = 33) and from control Thoroughbred mares (n = 4) with normal parturition for examination of transcriptional changes in the placenta associated with PPS. Transcriptomic changes in the villous CA near the cervical star were determined by Illumina® sequencing and subsequent bioinformatic analysis. PPS samples were divided by k-means clustering, and differentially expressed genes (DEGs) in each PPS cluster were identified by comparing to controls. Shared DEGs between PPS clusters were used for gene ontology analysis and pathway analysis.
Results: A total of 1204 DEGs were identified between PPS and control. Gene ontology revealed extracellular matrix (ECM) and cell adhesion, and pathway analysis revealed fatty acid, p-53, hypoxia and inflammation. Eleven key regulator genes of PPS including growth factors (IGF1, TGFB2, TGFB3), transcription factors (HIF1A, JUNB, SMAD3), and transmembrane receptors (FGFR1, TNFRSF1A, TYROBP) were also identified.
Main Limitations: The use of clinical history of PPS, in the absence of other criteria, may have led to misidentification of some cases as PPS.
Conclusions: Transcriptomic analysis indicated that changes in ECM and cell adhesion were important factors in equine PPS. Key predicted upstream events include genes associated with hypoxia, inflammation and growth factors related to the pathogenesis of equine PPS.
(© 2022 EVJ Ltd.)
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معلومات مُعتمدة: University of Kentucy; Albert G. Clay Endowment; Japan Racing Association
فهرسة مساهمة: Keywords: horse; placenta; premature placental separation; red bag; transcriptome
تواريخ الأحداث: Date Created: 20220527 Date Completed: 20230414 Latest Revision: 20230414
رمز التحديث: 20240628
DOI: 10.1111/evj.13602
PMID: 35622344
قاعدة البيانات: MEDLINE
الوصف
تدمد:2042-3306
DOI:10.1111/evj.13602