دورية أكاديمية
أعرض في EDS

JcTI-PepI, a synthetic peptide bioinspired in the trypsin inhibitor from Jatropha curcas, presents potent inhibitory activity against C. krusei, a neglected pathogen.

التفاصيل البيبلوغرافية
العنوان: JcTI-PepI, a synthetic peptide bioinspired in the trypsin inhibitor from Jatropha curcas, presents potent inhibitory activity against C. krusei, a neglected pathogen.
المؤلفون: Souza LAL; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Dias LP; Department of Biophysics, Paulista School of Medicine, São Paulo, SP, Brazil., Araújo NMS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Carneiro RF; Department of Fisheries Engineering, Federal University of Ceará, Fortaleza, CE, Brazil., Nagano CS; Department of Fisheries Engineering, Federal University of Ceará, Fortaleza, CE, Brazil., Teixeira CS; Center of Agricultural Science and Biodiversity, Federal University of Cariri, Crato, CE, Brazil., Silva RGG; Biology Department, Federal University of Ceará, Fortaleza, CE, Brazil., Oliveira JTA; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Sousa DOB; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil. Electronic address: daniele.sousa@ufc.br.
المصدر: Biochimie [Biochimie] 2022 Sep; Vol. 200, pp. 107-118. Date of Electronic Publication: 2022 May 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 1264604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1638-6183 (Electronic) Linking ISSN: 03009084 NLM ISO Abbreviation: Biochimie Subsets: MEDLINE
أسماء مطبوعة: Publication: Paris : Editions Scientifiques Elsevier
Original Publication: Paris.
مواضيع طبية MeSH: Anti-Infective Agents*/pharmacology , Jatropha*, Animals ; Chlorocebus aethiops ; Humans ; Mammals ; Microbial Sensitivity Tests ; Peptides/pharmacology ; Trypsin Inhibitors ; Vero Cells
مستخلص: Antimicrobial resistance has been increasing globally, posing a global public health risk. It has prompted the scientific community to look for alternatives to traditional drugs. Antimicrobial Peptides (AMPs) have stood out in this context because they have the potential to control infectious diseases while causing no or little harm to mammalian cells. In the present study, three peptides, JcTI-PepI, JcTI-PepII, and JcTI-PepIII, were designed and tested for antimicrobial activity based on the primary sequence of JcTI-I, a 2S albumin with trypsin inhibitory activity from Jatropha curcas. JcTI-PepI strongly inhibited C. krusei growth, and it caused severe disruptions in cellular processes and cell morphology. C. krusei cells treated with JcTI-PepI showed indicative of membrane permeabilization and overproduction of Reactive Oxygen Species. Moreover, the yeast's ability to acidify the medium was severely compromised. JcTI-PepI was also effective against pre-formed biofilm and did not harm human erythrocytes and Vero cells. Overall, these characteristics indicate that JcTI-PepI is both safe and effective against C. krusei, an intrinsically resistant strain that causes serious health problems and is frequently overlooked. It implies that this peptide has a high potential for use as a new antimicrobial agent in the future.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest.
(Copyright © 2022 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
فهرسة مساهمة: Keywords: AMPs; Antimicrobial resistance; C. krusei
المشرفين على المادة: 0 (Anti-Infective Agents)
0 (Peptides)
0 (Trypsin Inhibitors)
تواريخ الأحداث: Date Created: 20220527 Date Completed: 20220823 Latest Revision: 20220823
رمز التحديث: 20231215
DOI: 10.1016/j.biochi.2022.05.014
PMID: 35623496
قاعدة البيانات: MEDLINE
الوصف
تدمد:1638-6183
DOI:10.1016/j.biochi.2022.05.014