دورية أكاديمية
أعرض في EDS

Inhaled Corticosteroids Selectively Alter the Microbiome and Host Transcriptome in the Small Airways of Patients with Chronic Obstructive Pulmonary Disease.

التفاصيل البيبلوغرافية
العنوان: Inhaled Corticosteroids Selectively Alter the Microbiome and Host Transcriptome in the Small Airways of Patients with Chronic Obstructive Pulmonary Disease.
المؤلفون: Yip W; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; School of Biomedical Engineering, Faculties of Medicine and Applied Sciences, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6T 1Z3, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., Li X; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada., Koelwyn GJ; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Faculty of Health Sciences, Simon Fraser University, Burnaby, BC V5A 1S6, Canada., Milne S; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., Leitao Filho FS; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada., Yang CX; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada., Hernández Cordero AI; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada., Yang J; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., Yang CWT; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., Shaipanich T; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., van Eeden SF; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., Leung JM; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., Lam S; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada.; British Columbia Cancer Agency, The University of British Columbia, Vancouver, BC V5Z 4E6, Canada., McNagny KM; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; School of Biomedical Engineering, Faculties of Medicine and Applied Sciences, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6T 1Z3, Canada., Sin DD; Centre for Heart Lung Innovation, The University of British Columbia, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.; Division of Respiratory Medicine, Department of Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada.
المصدر: Biomedicines [Biomedicines] 2022 May 11; Vol. 10 (5). Date of Electronic Publication: 2022 May 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101691304 Publication Model: Electronic Cited Medium: Print ISSN: 2227-9059 (Print) Linking ISSN: 22279059 NLM ISO Abbreviation: Biomedicines Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: Background: Patients with chronic obstructive pulmonary disease (COPD) are commonly treated with inhaled corticosteroid/long-acting ß2-agonist combination therapy. While previous studies have investigated the host-microbiome interactions in COPD, the effects of specific steroid formulations on this complex cross-talk remain obscure.
Methods: We collected and evaluated data from the Study to Investigate the Differential Effects of Inhaled Symbicort and Advair on Lung Microbiota (DISARM), a randomized controlled trial. Bronchoscopy was performed on COPD patients before and after treatment with salmeterol/fluticasone, formoterol/budesonide or formoterol-only. Bronchial brush samples were processed for microbial 16S rRNA gene sequencing and host mRNA sequencing. Longitudinal changes in the microbiome at a community, phylum and genus level were correlated with changes in host gene expression using a Spearman's rank correlation test.
Findings: In COPD patients treated with salmeterol/fluticasone, the expression levels of 676 host genes were significantly correlated to changes in the alpha diversity of the small airways. At a genus level, the expression levels of 122 host genes were significantly related to changes in the relative abundance of Haemophilus . Gene enrichment analyses revealed the enrichment of pathways and biological processes related to innate and adaptive immunity and inflammation. None of these changes were evident in patients treated with formoterol/budesonide or formoterol alone.
Interpretation: Changes in the microbiome following salmeterol/fluticasone treatment are related to alterations in the host transcriptome in the small airways of patients with COPD. These data may provide insights into why some COPD patients treated with inhaled corticosteroids may be at an increased risk for airway infection, including pneumonia.
Funding: The Canadian Institute of Health Research, the British Columbia Lung Association, and an investigator-initiated grant from AstraZeneca.
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فهرسة مساهمة: Keywords: 16S rRNA gene sequencing; COPD; bronchoscopy; budesonide; fluticasone; inflammation; inhaled corticosteroids; mRNA-sequencing; microbiome; transcriptomics
تواريخ الأحداث: Date Created: 20220528 Latest Revision: 20220716
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9138653
DOI: 10.3390/biomedicines10051110
PMID: 35625847
قاعدة البيانات: MEDLINE
الوصف
تدمد:2227-9059
DOI:10.3390/biomedicines10051110