دورية أكاديمية
أعرض في EDS

Heterologous Expression, Purification, and Immunomodulatory Effects of Recombinant Lipoprotein GUDIV-103 Isolated from Ureaplasma diversum .

التفاصيل البيبلوغرافية
العنوان: Heterologous Expression, Purification, and Immunomodulatory Effects of Recombinant Lipoprotein GUDIV-103 Isolated from Ureaplasma diversum .
المؤلفون: Santos-Junior MN; Department of Biointeraction, Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista 40170-110, Brazil.; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil., Neves WS; Department of Biointeraction, Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista 40170-110, Brazil., Santos RS; Department of Biointeraction, Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista 40170-110, Brazil., Almeida PP; Bioinformatics and Computational Biology Lab, Division of Experimental and Translational Research, Brazilian National Cancer Institute (INCA), Rio de Janeiro 20231-050, Brazil., Fernandes JM; Department of Biointeraction, Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista 40170-110, Brazil., Guimarães BCB; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil., Barbosa MS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo 05508-000, Brazil., da Silva LSC; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil., Gomes CP; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil., Sampaio BA; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil., Rezende IS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo 05508-000, Brazil., Correia TML; Department of Biointeraction, Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista 40170-110, Brazil., Neres NSM; Department of Biointeraction, Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista 40170-110, Brazil., Campos GB; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil., Bastos BL; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil., Timenetsky J; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo 05508-000, Brazil., Marques LM; Department of Biointeraction, Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista 40170-110, Brazil.; Department of Biology, and Biotechnology of Microorganisms, State University of Santa Cruz (UESC), Ilhéus 45662-900, Brazil.; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo 05508-000, Brazil.
المصدر: Microorganisms [Microorganisms] 2022 May 16; Vol. 10 (5). Date of Electronic Publication: 2022 May 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: Ureaplasma diversum is a bacterial pathogen that infects cattle and can cause severe inflammation of the genital and reproductive systems. Lipid-associated membrane proteins (LAMPs), including GUDIV-103, are the main virulence factors in this bacterium. In this study, we heterologously expressed recombinant GUDIV-103 (rGUDIV-103) in Escherichia coli, purified it, and evaluated its immunological reactivity and immunomodulatory effects in bovine peripheral blood mononuclear cells (PBMCs). Samples from rabbits inoculated with purified rGUDIV-103 were analysed using indirect enzyme-linked immunosorbent assay and dot blotting to confirm polyclonal antibody production and assess kinetics, respectively. The expression of this lipoprotein in field isolates was confirmed via Western blotting with anti-rGUDIV-103 serum and hydrophobic or hydrophilic proteins from 42 U. diversum strains. Moreover, the antibodies produced against the U. diversum ATCC 49783 strain recognised rGUDIV-103. The mitogenic potential of rGUDIV-103 was evaluated using a lymphoproliferation assay in 5(6)-carboxyfluorescein diacetate succinimidyl ester−labelled bovine PBMCs, where it induced lymphocyte proliferation. Quantitative polymerase chain reaction analysis revealed that the expression of interleukin-1β, toll-like receptor (TLR)-α, TLR2, TLR4, inducible nitric oxide synthase, and caspase-3−encoding genes increased more in rGUDIV-103−treated PBMCs than in untreated cells (p < 0.05). Treating PBMCs with rGUDIV-103 increased nitric oxide and hydrogen peroxide levels. The antigenic and immunogenic properties of rGUDIV-103 suggested its suitability for immunobiological application.
References: Clin Vaccine Immunol. 2014 Feb;21(2):196-202. (PMID: 24334686)
Trop Anim Health Prod. 2017 Feb;49(2):311-316. (PMID: 27915439)
Theriogenology. 1984 Feb;21(2):295-301. (PMID: 16725880)
Toxicol Lett. 2011 Feb 25;201(1):15-26. (PMID: 21145380)
Can J Comp Med. 1980 Jul;44(3):252-8. (PMID: 7427772)
Vet Immunol Immunopathol. 2017 May;187:55-63. (PMID: 28494930)
BMC Res Notes. 2011 Oct 27;4:455. (PMID: 22032232)
Vet Res. 2012 Jan 26;43:7. (PMID: 22280251)
J Hyg (Lond). 1985 Oct;95(2):375-82. (PMID: 3934261)
Appl Microbiol Biotechnol. 2020 Mar;104(6):2411-2425. (PMID: 31993706)
BMC Vet Res. 2020 Oct 7;16(1):379. (PMID: 33028315)
Vet Microbiol. 2021 Apr;255:109013. (PMID: 33676093)
Vet Rec. 2009 Nov 7;165(19):572-3. (PMID: 19897874)
Int J Med Microbiol. 2018 Oct;308(7):819-828. (PMID: 30033344)
Biotechnol Bioeng. 1999 Nov 20;65(4):382-8. (PMID: 10506413)
Trends Cell Biol. 2001 Jan;11(1):6-10. (PMID: 11146277)
Nihon Juigaku Zasshi. 1979 Dec;41(6):629-37. (PMID: 522338)
Vet Microbiol. 2013 Dec 27;167(3-4):670-4. (PMID: 23993254)
Pol J Vet Sci. 2018 Sep;21(3):441-444. (PMID: 30468334)
Can J Vet Res. 1994 Oct;58(4):275-80. (PMID: 7889459)
Reprod Domest Anim. 2006 Dec;41(6):501-9. (PMID: 17107508)
Can Vet J. 1992 Jan;33(1):46-9. (PMID: 17423929)
Microb Pathog. 2018 Apr;117:225-231. (PMID: 29471139)
Genome Announc. 2015 Apr 16;3(2):. (PMID: 25883297)
J Biosci Bioeng. 2005 Apr;99(4):303-10. (PMID: 16233795)
J Med Microbiol. 1998 Aug;47(8):659-66. (PMID: 9877186)
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 15;851(1-2):51-70. (PMID: 16950667)
An Acad Bras Cienc. 2016;88 Suppl 1:643-52. (PMID: 26871498)
Curr Drug Targets Inflamm Allergy. 2005 Aug;4(4):471-9. (PMID: 16101524)
Can J Comp Med. 1978 Apr;42(2):151-5. (PMID: 352491)
Vet Microbiol. 2020 Dec;251:108866. (PMID: 33099078)
Front Immunol. 2021 Feb 15;12:619362. (PMID: 33659004)
Dev Comp Immunol. 2016 Feb;55:111-8. (PMID: 26499291)
An Acad Bras Cienc. 2017 Oct-Dec;89(4):2987-2996. (PMID: 29236848)
FEMS Microbiol Lett. 2001 Sep 11;203(1):115-23. (PMID: 11557149)
Front Immunol. 2020 Jul 31;11:1495. (PMID: 32849509)
Res Vet Sci. 1976 Sep;21(2):227-31. (PMID: 968191)
Emerg Infect Dis. 1997 Jan-Mar;3(1):21-32. (PMID: 9126441)
Aust Vet J. 2013 Mar;91(3):99-101. (PMID: 23438461)
PLoS One. 2014 Mar 24;9(3):e91549. (PMID: 24662769)
Vet Immunol Immunopathol. 2013 Sep 15;155(3):155-61. (PMID: 23928261)
Front Microbiol. 2018 Jul 31;9:1682. (PMID: 30108558)
Infect Immun. 2014 Jan;82(1):62-71. (PMID: 24126524)
Can Vet J. 2018 Jan;59(1):43-46. (PMID: 29302101)
Front Microbiol. 2014 Apr 17;5:172. (PMID: 24860555)
BMC Microbiol. 2016 Sep 23;16(1):222. (PMID: 27663303)
Vet Immunol Immunopathol. 2010 Jul;136(1-2):116-21. (PMID: 20189658)
Can Vet J. 2018 Dec;59(12):1333-1337. (PMID: 30532293)
Vet Res Commun. 2011 Jan;35(1):21-34. (PMID: 21104123)
Biol Res. 2014 Sep 04;47:38. (PMID: 25299837)
Zentralbl Veterinarmed B. 1992 Oct;39(8):553-62. (PMID: 1462720)
Protein Expr Purif. 2006 Apr;46(2):483-8. (PMID: 16290005)
Can J Comp Med. 1980 Jul;44(3):259-66. (PMID: 7427773)
J Gen Microbiol. 1981 Oct;126(2):365-9. (PMID: 7040599)
Bioeng Bugs. 2010 Mar-Apr;1(2):116-31. (PMID: 21326937)
Isr J Med Sci. 1984 Oct;20(10):950-3. (PMID: 6439681)
Front Vet Sci. 2021 Feb 18;8:572171. (PMID: 33681318)
PLoS One. 2016 Sep 07;11(9):e0161926. (PMID: 27603136)
J Clin Microbiol. 1995 Mar;33(3):680-3. (PMID: 7751376)
J Dairy Sci. 2020 Nov;103(11):10429-10445. (PMID: 32921448)
Vet Microbiol. 2016 Dec 25;197:39-46. (PMID: 27938681)
Anim Reprod Sci. 2000 Nov 1;63(3-4):137-43. (PMID: 10989224)
FEMS Immunol Med Microbiol. 2009 Jan;55(1):93-9. (PMID: 19076225)
J Dairy Sci. 2015 Feb;98(2):918-26. (PMID: 25434342)
Vet Microbiol. 2000 Mar 15;72(3-4):241-50. (PMID: 10727834)
Front Cell Infect Microbiol. 2017 Apr 13;7:123. (PMID: 28451522)
Mol Biol Evol. 2007 Aug;24(8):1596-9. (PMID: 17488738)
Vet Microbiol. 2019 Aug;235:53-62. (PMID: 31282379)
Vet Microbiol. 2011 Aug 26;152(1-2):205-11. (PMID: 21601382)
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027. (PMID: 33892491)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Microb Pathog. 2019 Jan;126:92-100. (PMID: 30385395)
Aust Vet J. 2013 Nov;91(11):469-73. (PMID: 24571302)
Brief Bioinform. 2014 Nov;15(6):953-62. (PMID: 23926206)
Inflamm Res. 2012 May;61(5):493-501. (PMID: 22270622)
Theriogenology. 1983 Sep;20(3):367-74. (PMID: 16725853)
BMC Vet Res. 2020 May 26;16(1):163. (PMID: 32456681)
Infect Immun. 1996 Feb;64(2):637-43. (PMID: 8550219)
Front Microbiol. 2018 Jul 12;9:1538. (PMID: 30050519)
Vet Microbiol. 2021 Sep;260:109182. (PMID: 34315003)
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D207-12. (PMID: 16381847)
Ann N Y Acad Sci. 1967 Jul 28;143(1):517-8. (PMID: 5233785)
معلومات مُعتمدة: 409758/2018-3 National Council for Scientific and Technological Development; Code 001 Coordenação de Aperfeicoamento de Pessoal de Nível Superior
فهرسة مساهمة: Keywords: Ureaplasma diversum; immunomodulation; lipoproteins; lymphoproliferation
تواريخ الأحداث: Date Created: 20220528 Latest Revision: 20230308
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9147684
DOI: 10.3390/microorganisms10051032
PMID: 35630474
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2607
DOI:10.3390/microorganisms10051032