دورية أكاديمية
أعرض في EDS

Serological and molecular epidemiology of leptospirosis and the role of dogs as sentinel for human infection in Nigeria.

التفاصيل البيبلوغرافية
العنوان: Serological and molecular epidemiology of leptospirosis and the role of dogs as sentinel for human infection in Nigeria.
المؤلفون: Pilau NN; Department of Medicine, Faculty of Veterinary Medicine, Usman Danfodiyo University Sokoto, Nigeria., Lubar AA; School of Medicine, University of California San Diego, United States., Daneji AI; Department of Medicine, Faculty of Veterinary Medicine, Usman Danfodiyo University Sokoto, Nigeria., Mera UM; Department of Medicine, Faculty of Veterinary Medicine, Usman Danfodiyo University Sokoto, Nigeria., Magaji AA; Department of Medicine, Faculty of Veterinary Medicine, Usman Danfodiyo University Sokoto, Nigeria., Abiayi EA; National Veterinary Research Institute, Vom, Plateau State, Nigeria., Chaiboonma KL; School of Medicine, University of California San Diego, United States., Busayo EI; Department of Theriogenology and Animal Production, Usman Danfodiyo University Sokoto, Nigeria., Vinetz JM; Department of Internal Medicine, Section of Infectious Diseases, Yale School of Medicine, United States., Matthias MA; Department of Internal Medicine, Section of Infectious Diseases, Yale School of Medicine, United States.
المصدر: Heliyon [Heliyon] 2022 May 21; Vol. 8 (5), pp. e09484. Date of Electronic Publication: 2022 May 21 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: London : Elsevier Ltd, [2015]-
مستخلص: Objective: Prospective cross-sectional study of dogs in Nigeria to study leptospirosis, inferred to be endemic in all regions of the country by researchers. Aim is to generate empirical updated evidence of leptospiral infection and delineate serovars involved.
Methods: Study determined the sero-prevalence and infection rate in 342 dogs using sero-assays, culture isolation and novel qPCR. In-house designed primers targeting conserved regions were used to amplify genes in quantitative Real-Time PCR (qRT-PCR) for leptospiral detection to serogroups. Molecular analysis of the leptospiral 16S rRNA and LipL 32 genes were used for identification of pathogenic Leptospira species. Primers targeting the O-antigen ( rfb ) region of the Leptospira lipopolysaccharide (LPS) were used for differentiating serovars based on comparative melting temperature (T m ) analysis against reference serogroups.
Results: Overall serological and bacteriological prevalence of 56 (16.4%) and 40 (11.7%) respectively was recorded. Vaccination, ages and season(s) were the strongest determinants of infection. Unvaccinated animals, stray dogs and symptomatic dogs presented statistically significant (P < 0.05) higher risk of infection: OR 25.531 (6.108, 106.712; 95% CI).
Discussion: The evidence suggests 1 of every 10 dogs is infected and could be symptomatic for the disease or a carrier of leptospires in the studied region in Nigeria with attendant public health risks.
Competing Interests: The authors declare no conflict of interest.
(© 2022 The Author(s).)
References: J Vet Med Sci. 2009 Sep;71(9):1191-9. (PMID: 19801899)
Pan Afr Med J. 2013 Dec 19;16:144. (PMID: 24711882)
PLoS Negl Trop Dis. 2010 Feb 23;4(2):e612. (PMID: 20186328)
Rev Sci Tech. 1989 Jan;8(3):733-745. (PMID: 32344960)
J S Afr Vet Assoc. 2010 Sep;81(3):156-9. (PMID: 21247041)
Prev Vet Med. 2002 Dec 30;56(3):203-13. (PMID: 12441236)
Int J Infect Dis. 2014 Nov;28:47-64. (PMID: 25197035)
Rev Sci Tech. 1990 Dec;9(4):1195-6. (PMID: 2132712)
Semin Vet Med Surg Small Anim. 1996 Aug;11(3):166-71. (PMID: 8942213)
Int J Infect Dis. 2008 Jul;12(4):351-7. (PMID: 18055245)
Philos Trans R Soc Lond B Biol Sci. 2009 Sep 27;364(1530):2777-87. (PMID: 19687045)
PLoS Med. 2006 Aug;3(8):e308. (PMID: 16933963)
Clin Microbiol Infect. 2011 Apr;17(4):494-501. (PMID: 21414083)
PLoS Negl Trop Dis. 2008 Apr 02;2(4):e213. (PMID: 18382606)
J Bacteriol. 2004 May;186(9):2818-28. (PMID: 15090524)
J Trop Med Hyg. 1993 Oct;96(5):301-4. (PMID: 8411305)
Int J Environ Res Public Health. 2014 Apr 17;11(4):4316-25. (PMID: 24747539)
Antimicrob Agents Chemother. 2013 Jan;57(1):297-302. (PMID: 23114772)
PLoS One. 2012;7(2):e32712. (PMID: 22393440)
فهرسة مساهمة: Keywords: Diagnosis; Epidemiology; Infection; Nigeria; Reservoirs; Zoonoses
تواريخ الأحداث: Date Created: 20220601 Latest Revision: 20220716
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9136256
DOI: 10.1016/j.heliyon.2022.e09484
PMID: 35647333
قاعدة البيانات: MEDLINE
الوصف
تدمد:2405-8440
DOI:10.1016/j.heliyon.2022.e09484