دورية أكاديمية
Kinetic Characterization of the Immune Response to Methicillin-Resistant Staphylococcus aureus Subcutaneous Skin Infection.
| العنوان: | Kinetic Characterization of the Immune Response to Methicillin-Resistant Staphylococcus aureus Subcutaneous Skin Infection. |
|---|---|
| المؤلفون: | Ridder MJ; Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Centergrid.412016.0, Kansas City, Kansas, USA., McReynolds AKG; Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Centergrid.412016.0, Kansas City, Kansas, USA., Dai H; Department of Pathology and Laboratory Medicine, University of Kansas Medical Centergrid.412016.0, Kansas City, Kansas, USA., Pritchard MT; Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Centergrid.412016.0, Kansas City, Kansas, USA., Markiewicz MA; Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Centergrid.412016.0, Kansas City, Kansas, USA., Bose JL; Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Centergrid.412016.0, Kansas City, Kansas, USA. |
| المصدر: | Infection and immunity [Infect Immun] 2022 Jul 21; Vol. 90 (7), pp. e0006522. Date of Electronic Publication: 2022 Jun 01. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Methicillin-Resistant Staphylococcus aureus* , Soft Tissue Infections* , Staphylococcal Infections* , Staphylococcal Skin Infections*/microbiology, Animals ; Cytokines ; Immunity ; Mice ; Mice, Inbred C57BL ; Staphylococcus aureus |
| مستخلص: | Staphylococcus aureus is a leading cause of skin and soft tissue infections (SSTIs). Studies examining the immune response to S. aureus have been conducted, yet our understanding of the kinetic response to S. aureus subcutaneous skin infection remains incomplete. In this study, we used C57BL/6J mice and USA300 S. aureus to examine the host-pathogen interface from 8 h postinfection to 15 days postinfection (dpi), with the following outcomes measured: lesion size, bacterial titers, local cytokine and chemokine levels, phenotype of the responding leukocytes, and histopathology and Gram staining of skin tissue. Lesions were largest at 1 dpi, with peak necrotic tissue areas at 3 dpi, and were largely resolved by 15 dpi. During early infection, bacterial titers were high, neutrophils were the most abundant immune cell type, there was a decrease in most leukocyte populations found in uninfected skin, and many different cytokines were produced. Histopathological analysis demonstrated swift and extensive keratinocyte death and robust and persistent neutrophil infiltration. Gram staining revealed subdermal S. aureus colonization and, later, limited migration into upper skin layers. Interleukin-17A/F (IL-17A/F) was detected only starting at 5 dpi and coincided with an immediate decrease in bacterial numbers in the following days. After 9 days, neutrophils were no longer the most abundant immune cell type present as most other leukocyte subsets returned, and surface wounds resolved coincident with declining bacterial titers. Collectively, these data illustrate a dynamic immune response to S. aureus skin infection and suggest a key role for precisely timed IL-17 production for infection clearance and healthy tissue formation. |
| References: | MMWR Morb Mortal Wkly Rep. 2019 Mar 08;68(9):214-219. (PMID: 30845118) Cell. 2018 Feb 8;172(4):784-796.e18. (PMID: 29358051) PLoS Pathog. 2015 Nov 05;11(11):e1005226. (PMID: 26539822) Immunity. 2011 Mar 25;34(3):327-39. (PMID: 21376639) Nat Commun. 2021 Aug 4;12(1):4700. (PMID: 34349124) Infect Immun. 2012 Jun;80(6):1987-95. (PMID: 22431645) Nat Microbiol. 2022 Jan;7(1):62-72. (PMID: 34873293) mBio. 2019 Feb 5;10(1):. (PMID: 30723124) PLoS Pathog. 2010 Oct 07;6(10):e1001133. (PMID: 20949069) Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10408-10413. (PMID: 16801559) Nat Immunol. 2014 Jan;15(1):45-53. (PMID: 24270515) PLoS One. 2013 Oct 02;8(10):e75103. (PMID: 24098366) Infect Immun. 2013 Dec;81(12):4478-89. (PMID: 24082072) J Eur Acad Dermatol Venereol. 2012 Aug;26(8):931-41. (PMID: 22214317) J Immunol. 2011 Jan 1;186(1):443-52. (PMID: 21131426) J Invest Dermatol. 2001 May;116(5):633-40. (PMID: 11348449) Infect Immun. 2000 Nov;68(11):6162-7. (PMID: 11035720) BMC Res Notes. 2016 Apr 12;9:216. (PMID: 27071769) Hum Vaccin Immunother. 2019;15(12):2980-2992. (PMID: 31149870) Front Immunol. 2018 Feb 01;9:93. (PMID: 29449841) J Immunol. 1997 Nov 15;159(10):5084-8. (PMID: 9366437) J Invest Dermatol. 1996 Mar;106(3):397-403. (PMID: 8648167) Infect Immun. 2020 Jul 21;88(8):. (PMID: 32513856) J Immunol. 2008 Apr 15;180(8):5558-68. (PMID: 18390740) Cell Host Microbe. 2021 Jun 9;29(6):930-940.e4. (PMID: 33852876) J Clin Invest. 2010 May;120(5):1762-73. (PMID: 20364087) Immunity. 2012 May 25;36(5):873-84. (PMID: 22560445) Cell Host Microbe. 2017 Nov 8;22(5):653-666.e5. (PMID: 29120743) Immunity. 2009 Jan 16;30(1):108-19. (PMID: 19144317) Clin Exp Immunol. 2003 May;132(2):209-15. (PMID: 12699407) Cell Mol Life Sci. 2011 Jul;68(14):2371-90. (PMID: 21573786) Diagn Microbiol Infect Dis. 2007 Jan;57(1):7-13. (PMID: 17059876) Front Immunol. 2021 Feb 25;12:627139. (PMID: 33732249) J Immunol. 2015 Mar 15;194(6):2735-45. (PMID: 25681348) Nat Rev Immunol. 2016 May 27;16(6):378-91. (PMID: 27231052) Front Immunol. 2018 Jan 05;8:1941. (PMID: 29379502) Am J Pathol. 2012 Oct;181(4):1327-37. (PMID: 22885107) Proc Natl Acad Sci U S A. 2019 May 28;116(22):10917-10926. (PMID: 31088972) Appl Environ Microbiol. 2016 Dec 1;82(23):6859-6869. (PMID: 27637878) mBio. 2019 May 14;10(3):. (PMID: 31088921) J Infect Dis. 2010 Oct 1;202(7):1050-8. (PMID: 20726702) J Invest Dermatol. 2004 Jan;122(1):95-102. (PMID: 14962096) Clin Diagn Lab Immunol. 2002 Nov;9(6):1165-8. (PMID: 12414745) Infect Immun. 2014 May;82(5):1813-22. (PMID: 24549328) J Invest Dermatol. 2011 Jan;131(1):125-32. (PMID: 20882039) J Immunol. 2018 Jan 15;200(2):657-668. (PMID: 29222165) Toxins (Basel). 2013 Jun;5(6):1140-66. (PMID: 23888516) PLoS One. 2010 Apr 14;5(4):e10146. (PMID: 20418950) Cell Host Microbe. 2017 Nov 8;22(5):667-677.e5. (PMID: 29120744) |
| معلومات مُعتمدة: | P20 GM113117 United States GM NIGMS NIH HHS; P30 GM103326 United States GM NIGMS NIH HHS; U54 HD090216 United States HD NICHD NIH HHS; P20 GM130418 United States GM NIGMS NIH HHS; P30 CA168524 United States CA NCI NIH HHS; R01 AI121073 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: MRSA; immune response; methicillin-resistant Staphylococcus aureus; skin infection; virulence |
| المشرفين على المادة: | 0 (Cytokines) |
| تواريخ الأحداث: | Date Created: 20220601 Date Completed: 20220725 Latest Revision: 20221202 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9302141 |
| DOI: | 10.1128/iai.00065-22 |
| PMID: | 35647662 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5522 |
|---|---|
| DOI: | 10.1128/iai.00065-22 |