دورية أكاديمية
أعرض في EDS

Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci.

التفاصيل البيبلوغرافية
العنوان: Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci.
المؤلفون: Lima ARJ; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Silva HGS; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil.; Departamento de Microbiologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, Imunologia E Parasitologia, São Paulo, SP, Brazil., Poubel S; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Rosón JN; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil.; Departamento de Microbiologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, Imunologia E Parasitologia, São Paulo, SP, Brazil., de Lima LPO; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Costa-Silva HM; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Gonçalves CS; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal Do Rio de Janeiro, IBCCF, CCS, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil.; Centro Nacional de Biologia Estrutural E Bioimagem, Rio de Janeiro, RJ, Brazil., Galante PAF; Centro de Oncologia Molecular, Hospital Sírio Libanês, São Paulo, SP, Brazil., Holetz F; Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil., Motta MCM; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal Do Rio de Janeiro, IBCCF, CCS, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil.; Centro Nacional de Biologia Estrutural E Bioimagem, Rio de Janeiro, RJ, Brazil., Silber AM; Universidade de São Paulo, São Paulo, SP, Brazil., Elias MC; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., da Cunha JPC; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil. julia.cunha@butantan.gov.br.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil. julia.cunha@butantan.gov.br.
المصدر: Epigenetics & chromatin [Epigenetics Chromatin] 2022 Jun 01; Vol. 15 (1), pp. 22. Date of Electronic Publication: 2022 Jun 01.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101471619 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-8935 (Electronic) Linking ISSN: 17568935 NLM ISO Abbreviation: Epigenetics Chromatin Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central
مواضيع طبية MeSH: Chromatin*/genetics , Chromatin*/metabolism , Trypanosoma cruzi*/genetics , Trypanosoma cruzi*/metabolism, Chromatin Assembly and Disassembly ; Gene Expression Regulation ; Proteomics/methods
مستخلص: Background: Genomic organization and gene expression regulation in trypanosomes are remarkable because protein-coding genes are organized into codirectional gene clusters with unrelated functions. Moreover, there is no dedicated promoter for each gene, resulting in polycistronic gene transcription, with posttranscriptional control playing a major role. Nonetheless, these parasites harbor epigenetic modifications at critical regulatory genome features that dynamically change among parasite stages, which are not fully understood.
Results: Here, we investigated the impact of chromatin changes in a scenario commanded by posttranscriptional control exploring the parasite Trypanosoma cruzi and its differentiation program using FAIRE-seq approach supported by transmission electron microscopy. We identified differences in T. cruzi genome compartments, putative transcriptional start regions, and virulence factors. In addition, we also detected a developmental chromatin regulation at tRNA loci (tDNA), which could be linked to the intense chromatin remodeling and/or the translation regulatory mechanism required for parasite differentiation. We further integrated the open chromatin profile with public transcriptomic and MNase-seq datasets. Strikingly, a positive correlation was observed between active chromatin and steady-state transcription levels.
Conclusion: Taken together, our results indicate that chromatin changes reflect the unusual gene expression regulation of trypanosomes and the differences among parasite developmental stages, even in the context of a lack of canonical transcriptional control of protein-coding genes.
(© 2022. The Author(s).)
التعليقات: Erratum in: Epigenetics Chromatin. 2022 Jul 6;15(1):25. (PMID: 35794678)
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معلومات مُعتمدة: MR/P027989/1 United Kingdom MRC_ Medical Research Council
فهرسة مساهمة: Keywords: Active chromatin; Cell differentiation; Chromatin remodeling; Epigenomics; FAIRE-seq; Gene expression control; Polycistronic transcription; Trypanosoma cruzi; Virulence factors; tRNA
المشرفين على المادة: 0 (Chromatin)
تواريخ الأحداث: Date Created: 20220601 Date Completed: 20220603 Latest Revision: 20230222
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9158160
DOI: 10.1186/s13072-022-00450-x
PMID: 35650626
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-8935
DOI:10.1186/s13072-022-00450-x