دورية أكاديمية
Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci.
العنوان: | Open chromatin analysis in Trypanosoma cruzi life forms highlights critical differences in genomic compartments and developmental regulation at tDNA loci. |
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المؤلفون: | Lima ARJ; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Silva HGS; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil.; Departamento de Microbiologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, Imunologia E Parasitologia, São Paulo, SP, Brazil., Poubel S; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Rosón JN; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil.; Departamento de Microbiologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, Imunologia E Parasitologia, São Paulo, SP, Brazil., de Lima LPO; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Costa-Silva HM; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., Gonçalves CS; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal Do Rio de Janeiro, IBCCF, CCS, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil.; Centro Nacional de Biologia Estrutural E Bioimagem, Rio de Janeiro, RJ, Brazil., Galante PAF; Centro de Oncologia Molecular, Hospital Sírio Libanês, São Paulo, SP, Brazil., Holetz F; Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brazil., Motta MCM; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal Do Rio de Janeiro, IBCCF, CCS, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil.; Centro Nacional de Biologia Estrutural E Bioimagem, Rio de Janeiro, RJ, Brazil., Silber AM; Universidade de São Paulo, São Paulo, SP, Brazil., Elias MC; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil., da Cunha JPC; Laboratório de Ciclo Celular, Instituto Butantan, São Paulo, SP, Brazil. julia.cunha@butantan.gov.br.; Centro de Toxinas, Resposta Imune E Sinalização Celular (CeTICS), Instituto Butantan, São Paulo, Brazil. julia.cunha@butantan.gov.br. |
المصدر: | Epigenetics & chromatin [Epigenetics Chromatin] 2022 Jun 01; Vol. 15 (1), pp. 22. Date of Electronic Publication: 2022 Jun 01. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101471619 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-8935 (Electronic) Linking ISSN: 17568935 NLM ISO Abbreviation: Epigenetics Chromatin Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : BioMed Central |
مواضيع طبية MeSH: | Chromatin*/genetics , Chromatin*/metabolism , Trypanosoma cruzi*/genetics , Trypanosoma cruzi*/metabolism, Chromatin Assembly and Disassembly ; Gene Expression Regulation ; Proteomics/methods |
مستخلص: | Background: Genomic organization and gene expression regulation in trypanosomes are remarkable because protein-coding genes are organized into codirectional gene clusters with unrelated functions. Moreover, there is no dedicated promoter for each gene, resulting in polycistronic gene transcription, with posttranscriptional control playing a major role. Nonetheless, these parasites harbor epigenetic modifications at critical regulatory genome features that dynamically change among parasite stages, which are not fully understood. Results: Here, we investigated the impact of chromatin changes in a scenario commanded by posttranscriptional control exploring the parasite Trypanosoma cruzi and its differentiation program using FAIRE-seq approach supported by transmission electron microscopy. We identified differences in T. cruzi genome compartments, putative transcriptional start regions, and virulence factors. In addition, we also detected a developmental chromatin regulation at tRNA loci (tDNA), which could be linked to the intense chromatin remodeling and/or the translation regulatory mechanism required for parasite differentiation. We further integrated the open chromatin profile with public transcriptomic and MNase-seq datasets. Strikingly, a positive correlation was observed between active chromatin and steady-state transcription levels. Conclusion: Taken together, our results indicate that chromatin changes reflect the unusual gene expression regulation of trypanosomes and the differences among parasite developmental stages, even in the context of a lack of canonical transcriptional control of protein-coding genes. (© 2022. The Author(s).) |
التعليقات: | Erratum in: Epigenetics Chromatin. 2022 Jul 6;15(1):25. (PMID: 35794678) |
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معلومات مُعتمدة: | MR/P027989/1 United Kingdom MRC_ Medical Research Council |
فهرسة مساهمة: | Keywords: Active chromatin; Cell differentiation; Chromatin remodeling; Epigenomics; FAIRE-seq; Gene expression control; Polycistronic transcription; Trypanosoma cruzi; Virulence factors; tRNA |
المشرفين على المادة: | 0 (Chromatin) |
تواريخ الأحداث: | Date Created: 20220601 Date Completed: 20220603 Latest Revision: 20230222 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9158160 |
DOI: | 10.1186/s13072-022-00450-x |
PMID: | 35650626 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1756-8935 |
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DOI: | 10.1186/s13072-022-00450-x |