دورية أكاديمية
Chromosome-specific retention of cancer-associated DNA hypermethylation following pharmacological inhibition of DNMT1.
العنوان: | Chromosome-specific retention of cancer-associated DNA hypermethylation following pharmacological inhibition of DNMT1. |
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المؤلفون: | Wiseman AK; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., Tiedemann RL; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., Fan H; Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX, 77030, USA., Shen H; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., Madaj Z; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., McCabe MT; Cancer Epigenetics Research Unit, Oncology R&D, GlaxoSmithKline, Collegeville, PA, USA., Pappalardi MB; Cancer Epigenetics Research Unit, Oncology R&D, GlaxoSmithKline, Collegeville, PA, USA., Jones PA; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA. Peter.Jones@vai.org. |
المصدر: | Communications biology [Commun Biol] 2022 Jun 02; Vol. 5 (1), pp. 528. Date of Electronic Publication: 2022 Jun 02. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]- |
مواضيع طبية MeSH: | DNA Methylation* , Neoplasms*/genetics, CpG Islands ; DNA ; Female ; Humans ; Male ; X Chromosome |
مستخلص: | The DNA methylation status of the X-chromosome in cancer cells is often overlooked because of computational difficulties. Most of the CpG islands on the X-chromosome are mono-allelically methylated in normal female cells and only present as a single copy in male cells. We treated two colorectal cancer cell lines from a male (HCT116) and a female (RKO) with increasing doses of a DNA methyltransferase 1 (DNMT1)-specific inhibitor (GSK3685032/GSK5032) over several months to remove as much non-essential CpG methylation as possible. Profiling of the remaining DNA methylome revealed an unexpected, enriched retention of DNA methylation on the X-chromosome. Strikingly, the identified retained X-chromosome DNA methylation patterns accurately predicted de novo DNA hypermethylation in colon cancer patient methylomes in the TCGA COAD/READ cohort. These results suggest that a re-examination of tumors for X-linked DNA methylation changes may enable greater understanding of the importance of epigenetic silencing of cancer related genes. (© 2022. The Author(s).) |
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معلومات مُعتمدة: | R35 CA209859 United States CA NCI NIH HHS |
المشرفين على المادة: | 9007-49-2 (DNA) |
تواريخ الأحداث: | Date Created: 20220602 Date Completed: 20220606 Latest Revision: 20220716 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9163065 |
DOI: | 10.1038/s42003-022-03509-3 |
PMID: | 35654826 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2399-3642 |
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DOI: | 10.1038/s42003-022-03509-3 |