دورية أكاديمية
أعرض في EDS

Chromosome-specific retention of cancer-associated DNA hypermethylation following pharmacological inhibition of DNMT1.

التفاصيل البيبلوغرافية
العنوان: Chromosome-specific retention of cancer-associated DNA hypermethylation following pharmacological inhibition of DNMT1.
المؤلفون: Wiseman AK; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., Tiedemann RL; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., Fan H; Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX, 77030, USA., Shen H; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., Madaj Z; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA., McCabe MT; Cancer Epigenetics Research Unit, Oncology R&D, GlaxoSmithKline, Collegeville, PA, USA., Pappalardi MB; Cancer Epigenetics Research Unit, Oncology R&D, GlaxoSmithKline, Collegeville, PA, USA., Jones PA; Department of Epigenetics, Van Andel Institute, Grand Rapids, MI, 49503, USA. Peter.Jones@vai.org.
المصدر: Communications biology [Commun Biol] 2022 Jun 02; Vol. 5 (1), pp. 528. Date of Electronic Publication: 2022 Jun 02.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]-
مواضيع طبية MeSH: DNA Methylation* , Neoplasms*/genetics, CpG Islands ; DNA ; Female ; Humans ; Male ; X Chromosome
مستخلص: The DNA methylation status of the X-chromosome in cancer cells is often overlooked because of computational difficulties. Most of the CpG islands on the X-chromosome are mono-allelically methylated in normal female cells and only present as a single copy in male cells. We treated two colorectal cancer cell lines from a male (HCT116) and a female (RKO) with increasing doses of a DNA methyltransferase 1 (DNMT1)-specific inhibitor (GSK3685032/GSK5032) over several months to remove as much non-essential CpG methylation as possible. Profiling of the remaining DNA methylome revealed an unexpected, enriched retention of DNA methylation on the X-chromosome. Strikingly, the identified retained X-chromosome DNA methylation patterns accurately predicted de novo DNA hypermethylation in colon cancer patient methylomes in the TCGA COAD/READ cohort. These results suggest that a re-examination of tumors for X-linked DNA methylation changes may enable greater understanding of the importance of epigenetic silencing of cancer related genes.
(© 2022. The Author(s).)
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معلومات مُعتمدة: R35 CA209859 United States CA NCI NIH HHS
المشرفين على المادة: 9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20220602 Date Completed: 20220606 Latest Revision: 20220716
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9163065
DOI: 10.1038/s42003-022-03509-3
PMID: 35654826
قاعدة البيانات: MEDLINE
الوصف
تدمد:2399-3642
DOI:10.1038/s42003-022-03509-3