دورية أكاديمية
Enterococcus faecalis and Candida albicans dual-species biofilm: establishment of an in vitro protocol and characterization.
| العنوان: | Enterococcus faecalis and Candida albicans dual-species biofilm: establishment of an in vitro protocol and characterization. |
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| المؤلفون: | Fiallos NM; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil., Aguiar ALR; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil., Nascimento da Silva B; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil., Rocha MFG; School of Veterinary, State University of Ceará, Fortaleza, Brazil., Sidrim JJC; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil., Castelo Branco de Souza Collares Maia D, Cordeiro RA; Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil. |
| المصدر: | Biofouling [Biofouling] 2022 Apr; Vol. 38 (4), pp. 401-413. Date of Electronic Publication: 2022 Jun 02. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9200331 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1029-2454 (Electronic) Linking ISSN: 08927014 NLM ISO Abbreviation: Biofouling Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2002- : Abingdon, Oxford : Taylor & Francis Original Publication: Chur ; New York : Harwood Academic Publishers, c1988- |
| مواضيع طبية MeSH: | Anti-Infective Agents* , Enterococcus faecalis*, Biofilms ; Candida albicans |
| مستخلص: | Enterococcus faecalis is the most important agent of persistent apical periodontitis, and recently, Candida albicans has also been implicated in periapical infections. This study aimed to optimize an in vitro E. faecalis and C. albicans dual-species biofilm protocol for endodontic research. Different physicochemical conditions for biofilm formation were tested. Susceptibility assays to antimicrobials, biochemical composition and an ultra-morphological structure analyses were performed. Reproducible dual-species biofilms were established in BHI medium at 35 °C, for 48 h and in a microaerophilic atmosphere. An increase in biomass and chitin content was detected after vancomycin treatment. Structural analysis revealed that the dual-species biofilm was formed by both microorganisms adhered to the substrate. The proposed protocol could be useful for the study of interkingdom relationships and help to find new strategies against periapical infections. |
| فهرسة مساهمة: | Keywords: Candida albicans; Enterococcus faecalis; dual-species biofilm; interkingdom |
| المشرفين على المادة: | 0 (Anti-Infective Agents) |
| تواريخ الأحداث: | Date Created: 20220603 Date Completed: 20220610 Latest Revision: 20220711 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1080/08927014.2022.2084612 |
| PMID: | 35655421 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1029-2454 |
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| DOI: | 10.1080/08927014.2022.2084612 |